enzyme assay 中文意思是什麼

Detection of antibodies against caprine arthritis - encephalitis virus - protocol of enzyme - linked immunosorbent assay

山羊關節炎-腦炎抗體檢測方法.酶聯免疫吸附試驗

Determination of diethylstilbestrol residues in fishery products enzyme linked immuno sorbent assay

水產品中己烯雌酚殘留量的測定.酶聯免疫法

Enzyme - linked immunosorbent assay for avian leukosis virus p27 antigen

禽白血病病毒p27抗原.酶聯免疫吸附試驗方法

On the other hand, - 6 fatty acid desaturase injection assay will be performed to check whether a metabolic pathway is established. methords of research three plasmids vector with expression elements are used to establish a eucaryotic expression vector by restriction enzyme cutting and ligation. this vector is used to pronucleus microinjection

本實驗以pegfp - n1質粒為骨架載體，用酶切連接的方法構建一個順序含有- actin啟動子、 fad2cdna 、 sv40polya加尾信號的真核表達載體，雙切線性化后回收，使用回收的表達載體經原核顯微注射生產轉基因小鼠。

Development and application of enzyme linked immuno sorbent assay technology

酶聯免疫吸附反應的技術進展及應用

Clone and enzyme - linked immunosorbent assay of human intestinal trefoil factor

人小腸三葉因子的克隆及酶聯免疫吸附檢測

Intestinal trefoil factor ; enzyme - linked immunosorbent assay elisa ; clone and expression

小腸三葉因子酶聯免疫吸附檢測克隆表達

Temporal patterns of vitellogenin ( vg ) synthesis and uptake as well as vitellin ( vn ) degradation in n. vitripennis, were studied using the method of enzyme - linked immunosorbent assay ( elisa )

採用elisa測定法檢測了麗蠅蛹集金小蜂卵黃原蛋白合成與攝取，及卵黃磷蛋白降解的時間動態。

Determination of aflatoxin b1 in feeds - enzyme - linked immunosorbent assay

飼料中黃麴黴毒素b1的測定酶聯免疫吸附法

The assay procedure is based on the bioreaction of the analyte nd. ab and enzyme labeled hrp - nd. ab competing for the nd. ag sites at the newly regenerated biocomposite surface

該分析過程的原理是：分析物nd ab與酶標分析物hrp - nd ab在已更新的生物組分表面上，競爭結合抗原（ nd

Method for the determination of tylosin residue in honey - enzyme - linked immunosorbent assay method

蜂蜜中泰樂菌素殘留量測定方法酶聯免疫法

Method for the determination of chloramphenicol residues in honey - enzyme linked immunosorbent assay method

蜂蜜中氯黴素殘留量的測定方法酶聯免疫法

The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。

It was testified that the antibody can special immunological recognition the protein gst - cpti and cptl by indirect enzyme linked immunosorbent assay ( elisa ). the coefficient of correlation is significant and the potency is more than 1 : 800

經間接elisa法檢測，抗體能與gst - cpti 、 cpti蛋白特異性結合，其相關系數達到顯著水平，效價1 800 。

Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry

三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質

The enzyme - linked immunosorbent assay ( elisa ) and high performance liquid chro - matography ( hplc ) analysis for detection of mc were optimized. the removal rates of mc by conventional water treatment processes were investigated through the laboratory study and the detection of mc in every process in meiyuan drinking water treatment plant. results showed that the prechlorination of eutrophic water led to the release of intracellular toxins to water phase

本文完善了mc的elisa和hplc分析方法，通過模擬試驗及水廠實測調查了富營養化太湖水中mc在常規凈水工藝中的去除特性，結果表明預氯化使藻細胞內的mc釋放出來，混凝沉澱對細胞外mc無去除作用，砂濾可去除17 . 2 40 . 4的細胞外mc和19 . 0 36 . 6的總mc ，加氯消毒對細胞外mc和總mc的去除率分別為30 45 . 3和30 51 . 7 。

Protocal of enzyme - linked immunosobent assay for trichinellosis of swine

豬旋毛蟲病酶聯免疫吸附試驗操作規程

Methods isolates were identified as acinetobacter calcoaceticus by using antibiotic susceptibility test, plasmid profiles, restriction enzyme fingerprinting assay and plasmid elimination method

方法對我院不動桿菌的感染進行調查，採用藥敏試驗、質粒抽提和限制性核酸內切酶分析法、質粒消除試驗。

Since there are more things in the bud tissue of the male flower, such as phenolic compounds, polysacchrides, proteins and some other secondary metabolites, three methods used to isolate rna are tested in this assay, which are the enzyme digesting methods of ctab, ctab - dna and sds - dna

克服了常規方法和試劑盒無法提取出富含酚類化合物、多糖和一些尚無法確定的次級代謝產物的白樺花芽組織rna的障礙，提出了3種白樺雄花芽組織rna的提取方法： ctab 、 ctab - dna酶消化法和sds - dna酶消化法。

There was not obviously objective protein band has been seen on sds - page. the enzyme assay showed a light activity of a - gal. the result showed that the a - gal gene was not well expression in this expression system

A gald gs 15講行j十發階規發附表達，對發酵產』物進行了s巳aoe丁匕泳，得到一條約為40kd的條帶，確認了ra刁a卜d得到了表達；並對產物進行酶活性檢測，證明了ra gal d只有酶活性。