ethidium bromide 中文意思是什麼
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The probe detection and pcr amplification are contemporary, without ethidium bromide staining and gel running and any post - pcr manipulations, so that the contamination risks are reduced. those two methods were used to detect successfully the primus necrotic ringspot virus from sample delivered by beijing airport quarantine bureau. the amplified fragment was cloned into the vector of pmd18 - t then sequenced
我們利用建立的雜交誘捕們pcr elisa和隊f pcr對北京機場檢疫局送檢的櫻桃樣品檢出了pnrsv ，同時把451hp的pcr產物克隆到ppo18 t載體上，通過序列測定，同源世最高的是genebank中pnrsv資料序號gi15750502gb山57046 -
The acrystalliferous and plasmid - free derivatives of bacillus thuringiensis were screened with ethidium bromide treatment, elevating growth temperature from 42 to 44 then to 46 by degrees, and treating it with 0. 05 % sodium docecyl sulphate ( sds ) as the plasmid - curing agent at 46
分別用溴化乙錠誘變處理、逐級升溫培養以及用sds處理等三種方法對蘇雲金芽胞桿菌無晶體（ cry ~ - ）和無質粒突變株進行了篩選研究。 -
Pcr products were detected on a 1. 5 % agerose gel, stained by ethidium bromide. the bands containing the amplified fragments were visualized under uv illumination
5經漠化已錠染色的瓊脂糖凝膠電泳后，紫外檢測儀下觀察擴增結果。 -
There is no acrystalliferous derivative was screened after treating 4. 5 g / ml ethidium bromide, but after elevating growth temperature of ybt - 1463 and other 8 bacillus thuringiensis parental strains to 42, 9 acrystalliferous derivative were obtained. a series of partially plasmid - cured derivatives were further obtained from 3 acrystalliferous derivatives originated from wild - type strain ybt - 1463 by elevating temperature to 44
用限量培養基和42培養9株野生菌株，結果篩選到9株cry ~ -突變株；繼續升溫至44來培養其中的三個cry ~ -突變株，得到內生質粒被進一步消除的突變株。 -
A final period of extension was carried out for 9 min and final holding at 4. the pcr products were resolved after electrophoresis in 2 % agarose gel with ethidium bromide. the pcr products were visualized while the gel was exposed to ultraviolet light
擴增產物在2瓊脂糖凝膠上電泳，用pcrmarker作分子量標準，紫外燈下觀察，最適pcr反應條件為擴增出最強的sry產物帶而無非特異性擴增帶出現時的反應條件。
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