flask culture 中文意思是什麼

flask culture 解釋
玻瓶培養
  • flask : n. 1. 瓶，長頸瓶。2. 【化學】燒瓶。3. （攜帶用）扁瓶。4. （打獵用的）火藥筒。5. 【機械工程】沙箱，砂型。
  • culture : n. 1. 教養；修養；磨煉。2. 文化，（精神）文明。3. 人工培養，養殖；培養菌，培養組織。4. 耕作；栽培；造林。vt. 使有教養。
  1. Glass. glassware for bacteriological use. culture flask called " legroux tube "

    玻璃.實驗室玻璃器皿. 「李柯氏管」培養瓶
  2. Shake - flask culture condition of lysine fermentation were studied with czl. appropriate feeding of organic niteogen sources was favorable for fermentation jn our experiment, the concentations of the yeast extract and beef extract were 15g / l and 25g / l. the initial ( nh02so4 and glucose concentrations suitable for fermentor production ranged form 55g / l and 150 - 180g / l respectively

    通過搖瓶發酵實驗初步確定czl的發酵條件為：初糖： 150 - 180g / l ，採用無機氮源和有機氮源配和使用，最佳組合是：硫酸銨質量濃度為55g / l ；酵母膏質量濃度為15g / l ，牛肉膏質量濃度為25g / l為最佳。
  3. In the thesis, we tried to transfer the novel non - lytic system to suspension culture in spinner flask

    另外一方面，有文獻利用桿狀病毒作為載具，將外來基因送入哺乳動物細胞表現，且不會對哺乳動物細胞的生長造成影響。
  4. 5. exploring fermentation synthesis of quinic acid ( qa ) using bioengineering strain under shake flask conditions synthesis of qa using recombinant e. coli constructs was examined in m9 accumulation medium. the result indicated that qa was produced from d - glucose in the culture supernatant, but it is necessary to explore further the consequences of fermentation in next work

    工程菌搖瓶發酵產酸初步觀察初步探索了奎尼酸工程菌在m9積累培養基中的發酵產酸條件，結果表明工程菌經直接發酵由葡萄糖產生了奎尼酸，但尚須進一步優化發酵條件提高產酸率。
  5. The viable cells after counting with trypan blue dye exclusion were then transferred to culture flask containing dmem medium in a density of 1 10 ^ 6

    方法無菌條件下，從6月齡紐西蘭白兔的膝關節囊內剪取滑膜組織，採用組織塊培養法和酶消化法分離滑膜細胞。
  6. Results obtained included : ( 1 ) mscs began to attach to culture flask after 24h cell inoculation by centrifugation method. cell proliferation was active after 3d. and mscs began to attach to culture flask only after 36h cell inoculation by erythrocyte splitting method

    研究結果顯示： （ 1 ）密度梯度高心法接種后24小時可見少數細胞貼壁， 3天後，細胞增殖活躍。
  7. Based on the flask - shaking fed - batch culture, the test of the fed - batch reaction which synthesized the extracellular trehalose was performed with strain l61 in 5 - liter bioreactor

    確定了流加合成胞外海藻糖的最適條件，在底物濃度1 ， ph6
  8. An artificial environment can be created within the confines of a test-tube, a flask, or a culture dish.

    試管、瓶子、培養皿都能夠提供它們所需要的人工環境。
  9. Different cultivation condition such as induced time, methanol concentration and ph of culture medium was studied at shake flask cultivation

    通過搖瓶培養初步摸索了誘導時間、甲醇濃度、培養基ph值等培養條件對mut ~ +重組菌株表達pap的影響。
  10. No trace of any newly expressed protein band was noticed in supernant as well as in the cells by sds - page, except the verification of the substitution of beta - galactosidase gene ( the lose of galactosidase protein band ), which is a selective marker of the wild - type virus. elisa test results suggested the expression of egf in cells, but not in culture supernant. the quantitative calculation suggested the expressed egf was about 6 - 7 u g ( as egf standard ) per flask ( 2 > < 106 cells ) in the cellular extract

    將重組病毒rbmbacph - egf以10moi感染bmn細胞， 72小時后收集培養細胞和上清；培養上清和經超聲波處理的細胞樣品elisa檢測發現胞內樣品中存在能與egf抗體免疫反應的產物，粗略估計表達量約6 7 g 2 10 ~ 6個細胞（相當于egf標準品） ；重組病毒rbmbacph - egf穿刺接種5齡家蠶幼蟲，每隔24h收集蠶血淋巴，經elisa檢測發現第4天表達量最高，根據egf標準曲線計算蠶血淋巴的表達量約32 g ml ； elisa定性實驗還發現正常蠶血也存在與egf抗體間交叉反應的物質。
  11. Shake - flask study on high - density culture condition of genetically engineered pichia pastoris

    高密度培養條件的搖瓶研究
  12. Research on the fermentation of carica papaye vinegar by shake - flask culture

    釀制木瓜果醋的研究
  13. Cells were centrifuged for 5min at 1000rpm and removed supernatant. the cell peller was resuspended in 1 ml culture media and divided into two glass flask. cell surface makers ( cd45, cd90 ) were detected by immunocy - tochemistry

    當傳到第五代時，取一瓶mscs用胰酶消化，以8x10vcm 『濃度接種在六孔板中的蓋玻片上，應用免疫細胞化學方法檢測cd45人d90的表達。
  14. Keeping the ph of the culture broth at 6. 0, the initial glucose concentration of l %, the strain could produce 14. 5g / l trehalose after reaction at 50 for 6 hours, the production of trehalose increased by 62. 2 % than that of flask - shaking culture

    0 ，溫度50c條件下，發酵6h ，可使海藻糖的合成總量達14 sg l ，比搖瓶反應的海藻糖合成量增加了62 2 。
  15. The vhb expression in the strain hv was investigated by co - binding assay. in flask culture, the strain hv had almost cell densities compared with e. colihms174 in the early phase of the growth

    在搖瓶中培養，發現在培養前期， hv的生長與無vhb基因的對照菌e . colihms174相差不多，到培養後期時， vhb基因整合菌hv才略有生長優勢。
  16. Comparison between microcarrier and ordinary flask methods in induction and culture of human mesenchymal stem cells into osteoblasts in vitro

    微載體技術與普通方法成骨誘導培養人骨髓間充質幹細胞的比較
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