gene deletion 中文意思是什麼

gene deletion 解釋
【生物學】基因刪除。

  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  • deletion : n. 1. 刪除。2. 刪除部分。3. （遺傳學上染色體的）缺失。
  1. In addition to avermectins, s. avermitilis produces oligomycin, a strongly toxic compound. gene deletion vector pxl05 was used to disrupt oligomycin polyketide synthase ( pks ) encoding genes ( olma ) in streptomyces avermitilis cz8 - 73, the producer of anthelmintic avermectins b and the cell growth inhibitor oligomycin. olma gene cluster in the chromosome was displaced by deletion allele on the plasmid via double crossover

    本研究以產阿維菌素b和寡黴素的阿維鏈黴菌cz8 - 73為出發菌株，構建了基因缺失載體pxl05 ，並將其轉入cz8 - 73中，通過缺失載體和染色體之間的同源雙交換，對染色體上長達90kb的寡黴素聚酮合酶（ pks ）基因簇（ olma ）進行了缺失。
  2. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  3. Gene deletion to decrease acetic acid yield of saccharomyces cerevisiaes

    6基因降低啤酒酵母產乙酸量
  4. Effect of avec gene deletion on avermectins production in streptomyces avermitilis

    基因缺失對產素調控的影響
  5. 2. the sequence of si gene from ibv - lx4 strain was consisted of 1614 bp from initiation codon atg to the possible cleavage site of spike glycoprotein, encoding for a 18 - amino signal - peptide with the n terminus of si protein and a polypeptide of 537 - amino acids. 19 highly conserved, potential glycosylation sites and 17 cysteines residues were characterized with si protein, homology analysis showe that there were gene deletion -

    S1基因：其全序列共1614bp （從起始密碼子atg到s前體蛋白裂解位點） ，編碼537個氨基酸，其氨基端有一編碼18個氨基酸的信號肽序列，第12 13位氨基酸殘基構成了信號肽的切割位點， 14 19位與111 124位氨基酸殘基為s1蛋白的跨膜區域。
  6. We clone a 1. 3kb promoter sequence of the homologous gene in arabidopsis by pcr. this promoter is shown to direct the specific expression of the reporter gene, b - glucuronidase ( gus ), in trichomes of arabidopsis. promoter deletion analysis reveal that the region from - 300 - - 1 bp is sufficient to direct trichome - specific expression

    對其進行缺失突變，構建5個缺失表達載體轉基因擬南芥，葉片gus定量測定分析表明- 300bp ? - 1bp序列就可以指導gus基因在表皮毛細胞中特異表達，說明這段序列可能含有指導此啟動子在擬南芥表皮毛細胞進行特異表達的核心序列。
  7. Microcalorimetric study on deletion mutagenesis of the gene promoter sequences from the extremely halophilic archaea

    極端嗜鹽古生菌啟動子序列缺失突變的微量熱研究
  8. Rinat b, hubert c, corvol p, et al. pcr detection of the insertion / deletion polymorphism of the human angiotensin converting enzyme gene j. nucl acids res, 1990, 20 : 1433

    和姬苓，王永福，楊國安，等.血管緊張素受體? 1基因多態性與腦血管病的關系j .臨床神經病學雜志， 2007 ， 20 : 12
  9. It is possible that exogenous dna fragment integerated into acceptor genome, changing gene base sequence or base deletion or base insertion, inducing to mutant at gene level

    這可能是外源dna片段整合進受體的基因組中，改變基因的順序或者引起基因堿基的缺失、插入，從而在基因水平上發生突變。
  10. " although the simplest model for a cn affecting gene actiity is where the ariant is a deletion of a gene or part of a gene, we found examples where actiity is affected from a distance, " commented barbara stranger, first author and post - doctoral fellow at the wellcome trust sanger institute. " this may occur when the cn reduces the effectieness of a region that works to switch the genes on or off

    變異拷貝數機制能通過改變特定基因的「劑量」 （即復制后的拷貝數量） ，藉助于裂解某個包含后續可翻譯成蛋白質密碼的基因的活性片斷或藉助于降解控制基因活性即類似開/關及在我們基因組中的調整開關的基因組中具有調控作用的基因片斷來影響基因活性。
  11. A human lymphotoxin deletion gene fragment which lacking n - terminal 27 amino acid residues of the protein was pcr amplified using a pair of designed primers and cloned into expression vector pet36b ( + ) to construct a fusion protein with cbd tag, resulting a recombinant plasmid pet36b - lt 27. the recombinant plasmid was transformed into host e. coli bl21 ( de3 ) plyss

    採用pcr的方法擴增出人淋巴毒素n端缺失27個氨基酸的缺失體基因片段，將此基因片段克隆至表達載體pet36b （ + ）上，與t7lac啟動子控制下的cbdtag構建成表達融合蛋白的重組質粒pe736b - lt 27 。
  12. Successfully cloned and constructed infectious full - length cdna of attenuated lapinized csfv chinese - strain ( derived from spleen ) could make us get pure rna virus genome of csfv c - strain, and further study and utilize mutation, deletion, insertion and substitution of csfv gene on dna molecular level

    中國豬瘟兔化弱毒（脾淋毒）全長感染性cdna的克隆和構建，可以使我們得到純粹的csfvc -株rna病毒基因組，在dna水平上研究和利用csfv的基因突變、缺失、插入和替換。
  13. Pfge analysis of these 21 blocked strains revealed a common chromosomal deletion in the 300kb asel fragment which might be responsible for antibiotic 5102 - iii biosynthesis. so the 300kb fragment was recovered and used as probe to hybridize with 10 - 22 genomic library. cosmids in this region were aligned and suitable fragments in this region were selected and used further for the construction of gene replacement plasmids

    以此片段為探針釣出了位於該區域的文庫克隆，並利用指紋印跡和雜交技術將這些文庫克隆排列起來，進而以位於該區域的不同位置的片段做臂構建了用於轉化和接合轉移用的基因置換質粒，並試圖通過基因置換將該區域置換下來，但尚未得到最終結果。
  14. The result of blastx shows that one orf is extracellular serine protease precursor gene ; 3 orfs function as regulatory genes ; 5 orfs are involved in secretion pathway ; 2 orfs are related to production of lps ; and the annotation functions of the other 3 orfs are not clearly related to extracellular protease prouduction. marker exchange method was used to study the relationship between the production of protease and the 3 orfs. a deletion mutant of xcc _ 4463 was constructed successfully

    Orf注釋表明，共中一個基因為胞外蛋白酶結構基因， 3個基因與合成調控有關， 5個與轉運分泌有關， 2個與lps合成有關，其餘3個orf的注釋功能與胞外蛋白酶的關系未見報道，為研究這3個orf與胞外蛋白酶產生的關系，採用同源雙交換orf缺失法進行了進一步驗證，成功地構建了xcc _ 4463缺失突變體，所得缺失突變體經檢測胞外蛋白酶減少，在寄主上致病性降低。
  15. On our conjecture, gene 3a, a highly conserved sequence, may be the determinant of the virulence of fmdv. changes in 3a have been associated with altered host range. a deletion in 3a has been associated with bovine attenuation of fmdv

    我們根據已有資料推測， 3a基因可能是口蹄疫病毒的毒力決定簇，它具有高度保守性，其變異和部分缺失對病毒在牛體內的致弱和宿主嗜性發生改變起重要作用。
  16. Genetic studies suggest that blocking the enzyme ' s activity will not lead to harmful side effects ; deletion of the gene encoding beta - secretase in mice eliminated a - beta formation in the rodents ' brains without causing any apparent negative consequences

    遺傳研究的結果暗示，阻斷這個酵素的活性，並不會造成有害的副作用；移除小鼠的分泌酶基因，小鼠的腦部就無法製造出a - ，但卻不會造成任何明顯的負面後果。
  17. The derivatives of phz2104 resulting from the disruption of the three putative orfs respectively by aada can not confer dnd phenotype on zx1. using erase - a - base system, a series of exoiii progressive deletion mutants were prepared for sequencing of add gene cluster

    將add基因簇亞克隆到測序載體pbluescript sk （ + ）上，採用exo缺失突變法得到了66個單向遞減缺失亞克隆，然後對這些克隆進行測序。
  18. ( 2 ) gene structure of the rh d - negative in chinese have polymorphism, especially in the individuals with c antigen, which accorded with the oriental character. d gene exon of chinese polymorphism expressed for intact, partial deletion and total deletion, and probably existed specific d ( nf ) ce haplotype

    中國人rhd基因結構具有多態性，特別是帶有c的個體，符合東方人具有完全缺失、部分缺失和不缺失三種情況，並可能存在特殊的d （ nf ） ce單體型。
  19. A series of molecular markers were designed according to the snps ( simple nucleotide polymophisms ) and the insertion / deletion polymophisms in arabidopsis database. with these molecular markers, fine - structure mapping of the ast gene was finished

    根據擬南芥數據庫中的snps （ simplenucleotidepolymophisms ）序列和插入缺失多態性（ insertion deletionpolymorphisms ）序列，設計了一系列分子標記。
  20. After the examination of pcr, one non - fluorescent cell clone had the same result as the plasmid ploxifn - a 1000bp product ; another non - fluorescent cell clone showed a 500bp product, a deletion reaction was thought to happen between the two lox sites in the genome under the reaction of cre recombinase so that the gfp gene and neo gene etc were cut

    經pcr檢測后，有一個不發光細胞克隆擴增出了與對照質粒ploxifn相同的約1000bp的條帶，表明發生了替換反應；另一個不發光細胞克隆認為是在cre重組酶的作用下其內部自行發生了剪切作用，剪切了gfp基因，從而只擴增出了載體序列和一個lox位點的約500bp的條帶。
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