gene insertion 中文意思是什麼
gene insertion
解釋
【生物學】基因插入。-
The results showed that the f fragment, 728bp in length, could be a new gene with a little homology to the genes coding for polyketide synthetase or fatty - acid synthetase and the b fragment, about 4kb in length, is inferred to have repeat sequences around tn5 insertion site, in which there is homology to the wa 314 right arm of the high - pathogeniciry island of yersinia enterocolitica. to reveal any pathogenicity of enterobacter cloacae b8 and its mutated strains b8b and b8f to animals, the experiment with mice was carried out
結果顯示， f片段長度為728bp ，與現有生物數據庫的blast比較分析，發現該序列僅有局部短於1oobp的區域與polyketide合成酶基因或與脂肪酸合成酶基因有低的同源性，推測為一新基因； b片段長約4kb ，序列拼接結果推測靠近tn5插入位點部位有重復序列，對b片段tn5遠端的部分序列進行blast比較，發現它與小腸結腸炎耶爾森氏菌的強毒力島有一定的同源性。 -
Some tendency of tn5gusa5 transposition were found that all preferred sites of tn5gusa5 in xcc 8004 genomic dna are in at - rich regions ; target sequences of tn5gusa5 have some features that the probabilities of guanine and cytosine are high respectively at the head and tail base of target sequence ; the level of gene transcription does not influence insertion density of tn5gusa5 significantly
結果表明， tn5gusa5插入位點有一定的規律性： tn5gusa5在xcc8004基因組上傾向于插入低gc含量（ 50左右的區域插入密度最高）區段；插入位點的靶序列有一定的特異性，在靶序列的首位鳥嘌呤出現的幾率高，而在靶序列的末位胞嘧啶出現幾率高； tn5gusa5的插入密度與該區段基因的轉錄水平無明顯關系。 -
Quinic acid, used shikimate pathway in e. coli, it is necessary to extend metabolic pathway by introduction of a heterogenous gene qutb into the host cell. double specific enzyme genes arog, qutb or three ones arog, qutb, arob were co - expressed in a single plasmid pbv220 to improve the enzymes " rate - limiting reactions. modifications of e. coli chromosome by both disruption of the arod gene and directed - site insertion of the arob gene resulted in the change of carbon flow redirected into the quinic acid biosynthesis branch
利用大腸桿菌莽草酸途徑合成新的代謝物奎尼酸，須在宿主細胞引入異源酶基因擴展代謝途徑；串聯表達酶基因，同時適量增加不同種屬的多個關鍵酶酶量，改善限速反應；利用同源重組進行基因整合和基因破壞，改造染色體結構定向改變微生物代謝途徑；目的是將碳代謝流最大程度的引向奎尼酸生成的方向。 -
In this study, the avermectin - producing strain streptomyces avermitilis was studied and the avermectin biosynthesis gene cluster in the genomic dna of streptomyces avermitilis s - 2 was altered by the method of gene engineering. insertion inactivation of aved gene in the cluster by introducing apramycin resistance gene into aved gene resulted in the disappearance of " a " components of avermectins. when avec gene was inactivated by the same way, four " 1 " components were lost and only " 2 " components, the potential precursor of ivermectin, were accumulated
將該基因簇中的aved基因通過插入外源的安普黴素抗性基因片段使其失活，導致發酵產物中4個a組分（不需要的組分）的消失；將基因簇中的avec基因通過同樣手段，使其失活，導致發酵產物中4個「 1 」組分的消失，而主要積累「 2 」組分（進一步改造可成為伊維菌素的前體b _ 2組分） 。 -
Rinat b, hubert c, corvol p, et al. pcr detection of the insertion / deletion polymorphism of the human angiotensin converting enzyme gene j. nucl acids res, 1990, 20 : 1433
和姬苓，王永福，楊國安，等.血管緊張素受體? 1基因多態性與腦血管病的關系j .臨床神經病學雜志， 2007 ， 20 : 12 -
It is possible that exogenous dna fragment integerated into acceptor genome, changing gene base sequence or base deletion or base insertion, inducing to mutant at gene level
這可能是外源dna片段整合進受體的基因組中，改變基因的順序或者引起基因堿基的缺失、插入，從而在基因水平上發生突變。 -
Conventional genetic engineering needs continual insertion of a stimulant to keep the new gene running
坎托說：這種捺跳開關是有意義的，因為它不需更多的調控。 -
Strain 042bm - x2 was found to contain a single tn5 insertion in the gene smc02682, which showing a high degree of similarity with the rhizobium tropici gshb gene, encoding the enzyme glutathione synthetase. in our experiment, this gene was demonstrated to be related to salt tolerance. so it is named as rst - 0x2
Rst - 0x1基因的功能未知，而rst - 0x2基因和熱帶根瘤菌谷胱甘肽合成酶基因gshb有很高的同源性。 pcr擴增得到包含啟動子的042bmrst - 0x1和rst - 0x2基因的全長。 -
I have used low copy pbin19 and single copy pmw755i5j binary vectors as backbone plasmids, to create a gene targeting insertion vector designated gfp tnos. after agro - infiltration into transgenic nicotiana benthamiana 16c, progeny were analyzed genetically for phenotypic changes, sirna accumulation, and dna methylation
採用農桿菌浸潤法（ agro - infiltration ）感染轉基因本生煙16c ，並對同源基因瞬時表達所引起的植物表型變化、小分子rna的產生、 dna甲基化程度、以及相關性狀在後代中的遺傳情況進行了檢查。 -
Finally, a tranfer vector named as pltk - ha was constructed based on pltk - uni with insertion of ha gene of h3 subtype srv. the pltk - ha and prv bartha - k61 genomic dna were co - transfected into 50 - 70 % confluent vero cells in 6 - cm dishes. based on the expression of lacz gene, recombinant prv were selected and purified by blue - colored virus plaque
利用脂質體介導的方法，將pltk - ha與prvbartha - k61基因組共轉染于亞單層vero細胞，依據報告基因lacz在細胞中的表達，篩選藍色蝕斑的重組病毒，經數次蝕斑純化后， pcr鑒定、 western - blot分析。 -
Successfully cloned and constructed infectious full - length cdna of attenuated lapinized csfv chinese - strain ( derived from spleen ) could make us get pure rna virus genome of csfv c - strain, and further study and utilize mutation, deletion, insertion and substitution of csfv gene on dna molecular level
中國豬瘟兔化弱毒（脾淋毒）全長感染性cdna的克隆和構建，可以使我們得到純粹的csfvc -株rna病毒基因組，在dna水平上研究和利用csfv的基因突變、缺失、插入和替換。 -
Substitution and insertion in all strains si gene, the homogeneity of nucleotide and the deduced amino acids of s1 gene with 17 alien and domestic references strains were equally less than 80 %
S1基因除與qx的親緣關系較近外，與其它參考株的同源率均低於80 ，可能為一株國內流行性的變異株。 -
Based on the blast analysis and other studies, osddl mutant was a multi - copy - insertion mutant, and one of the insertion sites was in an nptii like transposase gene, whereas osdd2, a single - copy - insertion mutant was disrupted at a gene encoded wrky transcript factor
Osdd1突變體可能是多拷貝插入，其中一個插入到nptii轉座酶類似基因。 osdd2突變體為單拷貝、插入到一個wrky類轉錄因子基因5翻譯起始區附近。 -
The e2 gene was amplified by rt - pcr, then examined the fragment by electrophoresis. after purification and insertion into pucm - t vecter, the recombinant plasmid pbne2pi and pbne2pii were obtained. then they were transfected e. coli jm109 and screened positive clones by blue or white plaques. the recombinant plasmids were extracted and the inserted fragments were identificated by electrophoresis
通過rt - pcr擴增e _ 2基因，電泳測定擴增片段的大小，經純化后，連接于pucm - t載體，獲得重組質粒pbne2p 、 pbne2p ，轉化e ? colijm109 ，經藍白斑篩選，挑取陽性克隆，提取質粒，直接電泳鑒定和酶切鑒定。 -
Phz621 - phz622, the new gene replacement vector system, had been constructed through the insertion of the 1. 0kb and 1. 4kb flanking sequences of hau3r gene from wild - type s. lividans in the same natural orientation
預期在攜帶有大插入片段的基因置換yac分子進入野生型變鉛青鏈黴菌后， yac分子將通過1 . 4kb或1 -
A series of molecular markers were designed according to the snps ( simple nucleotide polymophisms ) and the insertion / deletion polymophisms in arabidopsis database. with these molecular markers, fine - structure mapping of the ast gene was finished
根據擬南芥數據庫中的snps （ simplenucleotidepolymophisms ）序列和插入缺失多態性（ insertion deletionpolymorphisms ）序列，設計了一系列分子標記。 -
The gd and ge gene was subcloned into puc18, resulting in pugdge. the fragment from pcdnas. 1 - including hcmv promoter / enhancer, mcs and neomycin resistance gene was inserted into the bamhi and bsteii restrication sites of pugdge, resulting in the universal transfer vector pgd - m - ge. the universal transfer vector pgd - m - ge has deleted the gi gene and 363bp in the 5 ' end of the ge orf of prv. there were 11 restrication sites for insertion of the foreign gene. the upstream and downstream flanking sequences were up to 1. 25kb and 1. 42kb. it will be useful for developing the recombinant prv expressing foreign gene ( s )
將gd 、 ge基因連接于質粒puc18獲得pugdge ，缺失質粒pugdge的bamh和bste位點間391bp的片段。在此缺失位置插入來自質粒pcdna3 . 1 -的一偽狂犬病病毒gd 、 ge 、 tk基因的克隆與通用轉移載體的構建段含hcmv啟動子。多克隆位點和neo報告基因的片段，構建了通用轉移載體ppd m pe 。 -
Sequences flanking tn5 - 1063a can be recovered from the genome of mutant by excision, self - ligation and transfer to e. coli. the total dna of mutant was excised with ecori, which cut the genome frequently but not cut the transposon. after sequencing the self - ligated transpon, dna fragment flanking tn5 was obtained. the result showed 042bm - x1 contains a tn5 insertion in the gene smc00190, which function was unknown and was demonstrated to be related to salt tolerance by this study, and the gene was named as rst - 0x1
通過ecori酶切突變株基因組，得到完整的tn5 （含有在大腸桿菌中起始復制的oriv ）及其側翼的序列片段，該片段自連后轉化大腸桿菌，以tn5兩端已知的序列設計引物進行測序。 blast的分析測序結果表明， 042bm - x1和042bm - x2中tn5分別定位在苜蓿中華根瘤菌1021染色體上smc02682和smc00419基因內部，本實驗證明它們和042bm耐鹽相關，命名為rst - 0x1和rst - 0x2基因。 -
In addition, molecular experiments indicated that resistance was not relevant to copy number or to insertion sites, but instead to the repeated gfp structure and to methylation of genomic dna. pvx vectors were constructed that contained the s6 gene of rbsdv vi and the 14 kd fragment of bnyvv rna2
分子檢測結果顯示， gfp3植株中普遍存在gfp基因的沉默現象，且gfpdna的甲基化程度明顯高於gfp1植株，說明這些植株對于病毒的抗性與目的基因的插入位點及拷貝數和無關，但與串聯重復的gfp基因結構形式密切相關。 -
Association of insertion deletion polymorphism of the angiotensin - converting enzyme gene with congenital heart disease
先天性心臟病與血管緊張素轉換酶基因的關系探討
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