gene dose 中文意思是什麼
gene dose
解釋
基因分量-
In order to study the expression of 3 - defensins in liver as acute phase response proteins, a murine systemic acute phase responsive model was established by intraperitoneal injection o f lipopolysaccharide ( lps ) in our study. the mbd3 cdna sequence ( 145 - 169 bp ) was labled with [ - 32p ] atp as a probe to detect mbd3 mrna in different tissues by northern blot and analyze the time - and dose - dependant expression caused by lps. the 5 " flanking sequence ( - 167 - 179 bp ) of the mbd3 gene was designed as the probe and labled with [ - 32p ] dctp to investigate the binding of transcriptional factors to this region by electrophoretic mobility shift assay ( emsa ) and south - western blot
以小鼠mbd3基因145 ? 169bpcdna序列合成探針，經[ - ~ （ 32 ） p ] atp標記后通過northernblot方法檢測mbd3在肝臟中的表達，同時分析了mbd3基因誘導表達的組織特異性，劑效和時效關系；結合mbd3基因啟動子區序列分析，以- 164 ? - 179bp雙鏈dna序列合成探針，經[ - ~ （ 32 ） p ] dctp標記后通過電泳遷移率改變實驗（ emsa ）和south ? westernblot方法對參與mbd3在肝臟中誘導表達調節的轉錄因子進行分析。 -
The present studies aimed to find the responses of neurons and glias in the target and other brain areas of the linear accelerator - irradiated rats. in the first experiment, time - dependent neurons changes in the brain after hemispheric irradiation at an single - fraction maximal dose of 20 gy were observed by investigating the expression of an immediate early gene, fos protein. the results shown : compared with that of the un - radiated rats, the expression of fos protein in the irradiated brain decreased distinctly 24 hours and 1 week after irradiation
首先，利用即刻早期基因fos的表達，做了同一照射劑量（ 20gy ）照射大鼠半腦后不同存活期間（ 1d ， 7d ， 14d ， 2ld ， 28d ）大鼠全腦神經元動態變化的觀察，發現：受照射后1d 、 7d大鼠腦內各部位fos蛋白表達均明顯減少，隨著時間的延長，其fos免疫反應性細胞數量逐漸增加，照射后28d ，延髓、第四軍醫大學碩士學位論文腦橋內hs免疫陽性細胞數量恢復並超過正常對照組水平，但中腦、間腦及端腦內未恢復到正常對照組水平。 -
3. calcitonin gene - related peptide attenuates glutamate - induced inhibition of pulmonary surfactant lipid synthesis ( d cgrp could reverse the decrease of [ 3h ] - choline incorporation induced by glu with dose - dependence ; ( 2 ) cgrp could block the decrease of the content of cct a mrna induced by glu in lung tissue ; ( 3 } cgrp could reduce the impairment of the ultrastructure of at ii cells induced by glu ; ( 4 ) cgrp could reverse the increase of mda content and decrease of sod level induced by glu in cultured lung explants, respectively ; ? grp had no significant effect on nos activity and increase of no production induced by glu
降鈣素基因相關肽減輕谷氨酸所致肺表面活性物質脂質合成抑制的保護作用降鈣素基因相關肽grp ）可顯著減輕0所致肺組織h一膽堿摻入pc量的降低，並且呈劑量依賴保護效應； cgrp可逆轉gill所致cctqinrna含量的降低； cgrp可減輕o所致肺11上皮細胞超微結構的損傷； cgrp可逆轉o所致肺組織勻漿中mda含量增多、 sod水平降低的效應，並可逆轉q所致肺組織ldh釋放增多的效應； cg販對gill引起的nos活性和no含量的升高均沒有顯著性影響。 -
These results suggest that the transcriptional factor is the p50 - p65 heterodimer of nf - b. conclusion : 1. the expression of mbd3 gene can be induced in liver during the systemic acute - phase response to the challenge by lps ; 2. the acute phase - related mbd3 gene expression appears to be time - and dose - dependant of lps stimulation ; 3
結論: 1 . lps刺激引起小鼠急性時相反應可誘導mbd3基因在肝臟中表達; 2 . lps誘導的mbd3基因的急性時相表達具有劑量和時間依賴性; 3 . nf一『 b活化后參與mbd3基因誘導表達的轉錄調節。 -
In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers, the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e. coli m15. the following main results and conclusions can be obtained from the present study : 1. the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method, and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag. the recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease maping and sequencing, this expression vector might be instrumental to further study the function of axud1 protein in tumor cells
為了進一步研究axud1在人類腫瘤發生中的作用及axud1基因的表達狀況與tgf -介導的信號通路的關系，本實驗研究分為三個部分： （ 1 ） axud1基因cdna全長編碼區的克隆和ha表位標記的axud1基因表達載體的構建； （ 2 ）探討肝癌細胞hepg2和肺腺癌spc - a1細胞中tgf - 1誘導的axud1基因表達的時間、劑量效應以及誘導表達的可能機理，並研究axud1的過表達對細胞周期和細胞凋亡相關蛋白表達的影響； （ 3 ） axud1原核表達載體的構建及其在大腸桿菌中的表達。本實驗的主要結果和結論如下： 1利用一步法rt - pcr成功地從人類外周血淋巴細胞中克隆出axud1基因編碼區cdna ，並將其構建入真核表達載體中，編碼的ha - axud1融合蛋白帶有流感病毒凝血素ha的表位標記肽段。
分享友人