gene replication 中文意思是什麼
gene replication
解釋
基因復制- gene : n. 【生物學】基因。 dominant gene顯性基因。
- replication : n 1 （尤指對答辯的）回答；【法律】（原告對被告抗辯的）答辯。2 （繪畫等的）復制，摹寫；拷貝，復製...
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However, only a few host factors with clear in vivo function have been identified. by using pcr and 5 " and 3 " race, we were able to clone a homologue ( named ttom1 ) of arabdopsis thaliania host factor gene, tom1, which supports the replication of tobacco mosaic virus
根據從擬南芥中已經克隆的支持煙草花葉病毒復制的基因tom1序列設計一對特異性引物，用rt - pcr的方法獲得番茄同源基因的部分序列，然後利用5 』 race與3 』 race方法從番茄中克隆出全長ttom1基因。 -
Pseudorabies virus ( prv ) is the causative agent of aujeszky ' s disease, which results in significant losses in pig husbandry. thymidine kinase ( tk ) gene is one of the main virulent genes of prv, and it is essential to the propagation of prv in the nerve tissues, but dispensable for virus replication and infection in other tissues
偽狂犬病是由偽狂犬病病毒（ pseudorabiesvirus ， prv ）引起的家畜和多種野生動物的一種以發熱、奇癢、呼吸和神經系統疾病為特徵的急性傳染病，妊娠母豬可發生死胎和流產，是危害養豬業的一個重要傳染病。 -
It has been reported that the eiav s2 is not essential and does not appear to affect virus infection and replication in vitro. thus, we introduced a his - tag into the s2 gene of an eiav infectious molecular clone recombinant plasmid ( pok8266 ) by using soe pcr method and obtained a new recombinant plasmid with his - tag, designated as eiav - pok8. 2 - his
本研究應用已構建好的eiav驢白細胞弱毒疫苗株的感染性分子克隆載體（ pok8266 ）為模板，通過soepcr方法在感染性分子克隆載體的s2基因獨特區內引入突變點，形成含有酶切位點（ nspv ）的突變體（ p1p4 ） 。 -
In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv
本研究採用脂質體轉染方法，將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞，經6輪蝕斑克隆、篩選、表達， pcr鑒定和dot - elisa檢測，證明該重組病毒已構建成功，並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。 -
In e coli, dna polymerases are key enzymes involved in two distinguished pathways contributing to untargeted mutagenesis. replication of dna by pol v ( umuc ), in the presence of umud1, reca and single strand binding protein ( ssb ), is highly mutagenic and exhibits a predominant mutation pattern of base transversion. another error prone polymerase involved in untargeted mutagenesis is pol iv, encoded by dinb gene
在umud ' ， reca和單鏈結合蛋白ssb的協助下， polv （ umuc ）能在單鏈模板上催化dna合成並產生高頻率的以堿基顛換為主要形式的突變；另一個與非定標性突變有關的易誤dna聚合酶是pol ，為dinb基因的編碼產物。 -
3. using clamp technique, ex vivo gene transfer into liver graft was performed during cold preservation via perfusion of the portal vein with 5ml ringer ' s solution containing replication - defective adenovirus vector adhuctla4 - ig
供肝冷保存時，採用血管夾技術lamptechnique ）經門靜脈灌注攜帶融合基因hllctla4dg的重組腺病毒，于術后3天、 7天能定性檢測到hllctla4ig在受體外劃血卜1 。 -
Molecular epidemiology analysis of hiv in shenzhen and replication analysis between hiv - env gene variation and epidemic time
膜蛋白基因變異與感染時間相關性分析 -
The inhibitory effects on hepatitis b virus replication by stable expression of dn mutants of hepatitis b virus x gene prev x - gfp
融合基因表達載體的構建與在視網膜色素上皮細胞的表達定位 -
Hcv ns5b protein is encoded by ns5b gene, an rna - dependent rna polymerase that is responsible for the viral rna synthesis. due to no corresponding enzymes in the host cell and the importance of ns5b protein in the replication, ns5b protein has been regarded as an ideal target of anti - hcv infection
Hcvns5b基因編碼的ns5b蛋白，具有rna依賴的rna聚合酶活性（ rdrp ） ，是基因組復制的關鍵酶。出於ns5b蛋白在hcv基因組復制中的重要作用和宿主細胞中缺乏具有類似功能的相應酶類， ns5b蛋白是抗hcv研究中的理想靶位。
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