genomic blotting 中文意思是什麼
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The chimeric gene was introduced into arabidopsis through the method of vacuum infiltration which is widely used in arobidopsis tansformation. transgenic plants were screened by means of kanamycin resistance and further identified by genomic pcr and western blotting, through the phenotype observation of transgenic plants, the speculation that apoplast calmodulin may play a certain role in regulating the development and growth of plants was made
最後，融合基因被插入二元載體plbj21 ，通過電擊法轉入農桿菌gv3101 ，真空滲入法轉入了擬南芥，轉基因擬南芥通過kan抗性篩選而得到，並進一步進行了基因組pcr鑒定和western雜交的鑒定。 -
In the first part of this experiment, we isolated the tsmt gene ( genebank accession number : af499726. 1 ) from a a zap - cdna library cons tructed from a 200mmoll - 1 nacl - treated library of thellungiella salsuginea and analyzed its sequence characterization, genomic organization and the differential expression in response to salt stress. tsmt encodes metallothionein and there is only one copy in thellungiella salsuginea genome according to its southern blotting
L ~ （ - 1 ） nacl處理的鹽芥地上部分構建的zap - cdna文庫中克隆了編碼金屬硫蛋白的基因tsmt （ genebank號為af499726 . 1 ） ，並分別對其序列特徵、基因組結構和在鹽脅迫下的表達特性進行了分析，結果表明tsmt編碼鹽芥金屬硫蛋白， southern結果顯示tsmt在鹽芥基因組中只有一個拷貝。
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