high titer 中文意思是什麼

high titer 解釋
高效價,高滴度
  • high : adj 1 高的〈指物，形容人的身高用 tall〉；高處的；高地的。2 高級的，高等的，高位的，重要的。3 高尚...
  • titer : n. 1. 【化學】滴定量；滴定（濃）度，滴定率；效價；脂酸凍點（測定）。2. 【紡織；印染】纖度。
  1. Analysis of high - titer cold agglutinins causing cross - matching test difficult

    高效價冷凝集素致交叉配血困難分析
  2. In recent years, the outbreak of nd appear some new characters, it is reported that the amount of immunity defeat on nd increased, even chicks with high hi titer can be infected by ndv virulent strain. the gene - engineering vaccine is badly needed to control nd outbreak. so newcastle disease virus strain b95, a naturally avirulent and heat stable strain, was introduced from australia

    新城疫是國際獸疫局公布的16種烈性傳染病之一。近年來，新城疫的爆發出現了許多新特點，免疫失敗報道增多，甚至抗體水平較高的雞群也發病。為控制新城疫的爆發，急需研製基因工程苗。
  3. One group was immunized with cdv vaccine, and the other group was cav. serum samples and eggs were collected weekly for detected antibody titers. high titer egg yolk was collected for following igy extraction

    分別用cdv 、 cav的疫苗免疫產蛋雞，每周檢測雞血清和卵黃中的抗體水平，收集抗體水平高的雞蛋用於提取純化卵黃抗體igy 。
  4. Results the conjugation rate, high molecular conjugate content of and the antibody titer induced with pneumococcus type 1 conjugate vaccine prepared by cyanogen bromide activation were significantly higher than that by amine reduction

    結果採用溴化氰活化法制備的結合物，較胺還原法具有較高的結合率及高分子結合物含量，免疫小鼠產生的抗體效價較胺還原法明顯提高。
  5. In this study, we at first aimed at obtaining the gene encoding the specific ige antibody related proteins by immunoscreening the schistosoma juponi cum adult worm cdna library with the pooled high - titer ige antisera from the individuals living in the schistosome epidemic regions

    J測定。 jnij序結果顯示，該插入片段為1200hp ，第一開讀框長507hp ，編碼169個氨基酸殘基，理論分子量為19 3kdi 。
  6. Results we construct recombinant angiostatin baculovirus with a high virus titer ( 2 + 108 pfu / ml ) successfully. recombinant angiostatin was effectively expressed in insect cells ( sf9 ) as 53 kd fusing protein and its expression level was about 90 % of insect cellular total soluble proteins. the recombinant angiostatin protein could inhibit endothelial cell proliferation in vitro with ic50 value of 2

    實驗結果我們成功地構建了滴度高達2x10 『 pm llil的angiostatin重組? 2 ?桿狀病毒，並在昆蟲細胞sffi中高效表達了分子量為53kd的an giostatin重組蛋白，重組angiostatin蛋白不僅在體外顯著抑制內皮細胞的生長， k 。
  7. The purposes of this study is to find good methods for getting a quantity high titer serum and immunoglobulin, decrease the produce cost and control the infectious diseases

    特種經濟動物因為其本身的經濟價值很高，一旦發生傳染病損失慘重，因此有必要對患傳染病的動物進行治療和緊急預防，將損失降到最小。
  8. Constructing cdna expressing library of erythrocytic plasmodium falciparum from hainan : the total rna was obtained by using. triplix kit. a modified oligo ( dt ) primer ( cds ffi pcr primer ) was used in the single - stranded ( ss ) dna synthesis reaction. the ss - dna was reversely transcripted from total rna. double - stranded ( ds ) cdna was amplified by long - distance ( ld ) pcrafter the digestion with proteinase k and sfi i, the cdna with no less than 200bp was collected and purified by glass - milk kitthe library was constructed after the ligation of cdna to tiplex2 phage particle packaged with the packaging extract system in vitro. a high titer and high recombinant ratio of cdna library was constructed

    構建惡性瘧原蟲海南株紅內期cdna表達文庫提取紅內期惡性瘧原蟲海南株總rna ，直接以總rna為模板使用cdna文庫構建試劑盒，首先反轉錄合成ss一dna ，再擴增合成ds一dna （ cdna ） ，對擴增產物用蛋白酶k消化及左z丁i酶切，抽提蛋白、去除rna后，用玻璃奶試劑盒純化、回收20obp以上的片斷，經與載體連接再用蛋白包裝物包裝后形成未擴增文庫，最後擴增完成惡性瘧原蟲海南株紅內期cdna表達文庫的構建。
  9. Streptomyces tenebrarius a04, the producer ( with a titer of 2874u / ml ) of single component of nebramycin ? apramycin was studied in this paper. after treatment of spore suspension and mycelia shivered by supersonic with mutagen, combined with the application of screening models, some stable high yield apramycin - producing strains ( with a fermentation titer of 4800 - 5200u / ml by shaking flask ) such as a2 - 23, a2 - 30, asm6 and al - 16 were obtained

    本文以尼拉黴素單一組分?安普黴素產生菌s . tenebrariusa04 （發酵單位為2847u ml ）為出發菌株，通過對單孢子懸液和超聲波破碎菌體的誘變處理並復合篩選模型，獲得了遺傳特性穩定的單組分高產菌株： a2 - 23 、 a2 - 30 、 asm6 、 a1 - 16 ，搖瓶發酵單位達4800 - 5200u ml 。
  10. Once the recombinant virus has been i - dentified, we amplified the p - 1 stock to attain the large scale, high - titer viral stock in order to initiate expression studies. the recombinant angiostatin was produced from spodoptera frugiperda 9 ( sf9 ) insect cells infected by the high titer virus stock we have prepared. the time course for expression of recombinant protein was detected by sds - page and western blot, which can determine the optimal multiplicity of infection ( moi ) and the appropriate time of harvest for the protein

    表達重組蛋白angiostatin ：用制備好的帶有矗s標記的融合性angiostatin基因的高滴度重組桿狀病毒貯存液感染草地貪夜蛾sfg細胞，用sds page電泳和hsternblot對感染不同時間後分泌的重組蛋白做時間表達分析，依此確定最適的感染復數（ moi ）和感染時間，以達到重組蛋白表達水平最適化，而後大規模進行重組蛋白的表達， sds page用來分析重組蛋白， westernblot用來在蛋白表達水平低的情況下檢測表達的特異重組蛋白。
  11. The results show that the high hcv antibody titer of sows last whole year and the program that the sows are vaccinated two times a year and four doses one time is the best program of sows

    結果表明，每年免疫2次， 4頭份次，可使懷孕母豬的豬瘟抗體全年均維持在較高水平，此程序為繁殖母豬的最佳免疫程序。
  12. The target proteins with molecular weights of 35kd and 44kd were detected by sds - page. the 35kd target protein recovered from sds - page was used to immunize rabbit and the annsemm with high titer was obtained

    Annfaf基因在大腸桿菌中以包含體的形式得到了高效表達， sds - page檢測表明，分別獲得44kd和35kd大小的蛋白帶，與預期結果相同。
  13. 1. cloning of the gene encoding the specific ige antibody related antigen of schistosoma japonicum the serum samples from the inhabitants living in the epidemic regions of schistosomaisis japonica were collected and detected by abc - elisa method for their swap specific ige antibody. the sera with high titer of the specific ige levels were chosen and pooled

    89 ，其親水性、抗原性和可及性分佈曲線基本一致，預測抗原表位分另ij在第25 35位、 75 82位、 1h 1們位、 117 1n位和149 159位氨基酸殘基。
  14. The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2, ha order to prepare ndrg2 antibody, the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector. after induced by iptg, the fusion proteins were expressed in e. coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization, rabbits produced high titer antiserum against ndrg2. then antisemm was absorbed using ndrg2 antigen immobilized on nc filters, the purified product of antiserum shows high special to ndrg2 protein, and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach

    為制備高效價的ndrgz抗體，分別構建了prset a雌、 pgex4t d唾倉和pgex4tl七三種原核重組表達質粒，並在大腸桿菌中誘導表達出相應的融合蛋白；用全長gstjqdrgz蛋白免疫兔，然後用gst ndrgz人和gstjqdrgze片段加強免疫，經免疫得到了較高效價的兔抗人ndrz多克隆抗血清，利用固定於硝酸纖維素膜上的ndrgz抗原親和吸附純化抗血清，提高了ndrgz抗體的特異性；並對包涵體形式表達的6his ndrgz進行初步的分離純化。
  15. Whole immunization period went on 7 weeks, for that time primary and boosted immunization were carried out at 0, 2, 4, 7 week, respectively. the results shown that antibodies titer of immunized animals were 0. 54 and 0. 61 ( od ) at 4, 7 week, respectively, and control animals had no production of specific antibodies. high levels antibodies in plasma of immunized animals draw on large numbers of free ss, and its titer or integrated free ss concentration were 0. 48 - 0. 72 or 2. 4 - 11. 3nmol / l, respectively

    血液內高水平的特異性抗體結合了大量的游離ss （抗體滴度在0 . 48 - 0 . 72的范圍內，結合的ss的濃度范圍為2 . 4 - 11 . 3nmol l ） ，在不改變生長激素脈沖分泌各項指標和垂體生長激素mrna豐度的情況下（對照組和免疫組動物生長激素水平總平均，基線平均，峰頻率，峰強度和mrna豐度分別為3 . 14ng ml ， 2 . 25內豪古農業大學搏士學位論文ng ml ， 4
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