hybrid protein 中文意思是什麼
hybrid protein
解釋
雜化蛋白-
The targeted disruption of mouse rbp - j revealed that homozygous null mutants die before 10. 5 days postcoitum ( dpc ) with various abnormalities, including growth retardation and defects in the central nervous system and somites. in order to assess the function of rbp - j, a yeast two - hybrid screening using rbp - j as a bait protein was performed and a nuclear matrix protein, mint was isolated
Sdpc胚胎和人的hela細胞cdna文庫中篩選到一個可以與rbp斗相互作用的新蛋白ram7 ，后發laram7與mmt （ msxz inter肌tingnucleartargetprotein ）為同一蛋白。 -
To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei, and to study the function and mechanism of mint - mediated transcription repression, yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5, amino acids 2226 - 2959 ). from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo, fifty - one were positive for nutritional screening and p - galactosidase assay. restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments, which are mint, alpha a - crystallin - binding protein i ( alphaa - crybpl ), and nuclear receptor co - repressor 1 ( n - corl ), respectively
鑒于mwt基因編碼區較長，共有10799個堿基，故此我們將mint分為六段，分別命名為fi一f6 ，本研究以其中的f5 （ 222e959 ）和f6 （ 296s576 ）片段為研究對象，將h者分別插入pgb盯7載體中，結果顯示： mintfs可與核受體輔助抑制因子1階cori ） ， o晶狀體蛋白結合蛋白1hlphatcrybp入及mw加互作用，而f6可與igm的重鏈恆定區、泛素結合酶2l6和一個未知功能的新的蛋白基因進行結合。 -
The expressed product was lysised with supersonic wave and purified by sds - page. elisa analysis revealed that the antigenicity of the vpi protein has been detected. the forth part - detection of aev - nh937 strain by in situ hybridization ( 1sh ) - probe was labelled with digoxigenin ( dig ). then the probe hybrid with 5d, 10d, and 20d postinfection brain tissue of chicken. the results of the ish showed that the positive signal was found in 3 cases, while control group was negative. there has been a reasonable correlatien between this method and other detection test
經超聲波裂解，用尿素溶解包涵體，電泳純化后，利用elisa檢測vp _ 1外殼蛋白，表明具有一定抗原性；第四部分：應用原位雜交檢測aevi用dig標記的探針與sd 、 10d 、 20d的攻毒雞腦組織雜交，來檢測那v的rna 。 -
Further we cloned full - length cdna of the ked like protein coding region via pcr amplification and confirmed its interaction with acam2 in yeast two - hybrid system
進一步通過rt . pcr克隆了ked樣蛋白編碼區全長cdna ，通過雙雜交驗證了全長ked樣蛋白與acamz的相互作用，結果為陽性。 -
3 fifty candidates proteins possible interacting with the nthk1 protein were screened from the tobacco cdna library through the yeast two - hybrid system
3利用酵母雙雜交技術從煙草的cdna表達文庫中篩到50個可能與nthk1的相互作用的蛋白基因。 -
Nor protein partner interacting with pgl29 was detected by yeast two - hybrid screening. thus further work needs to be made to understand the fu
酵母雙雜交篩選結果，也未得到特異地與pgl29相互作用的直白質分于，因而現有的實驗無法確定該基因的功能。 -
Pit13, an interactor binding to trpt1 protein, was screened by yeast two - hybrid, and confirmed by pull down and co - ip assays. the association of trpt1 with pit 13 demonstrated that trpt1 should probably participate in other activities besides in pre - trna splicing. more experiments will be required to determine the role of pit 13 protein
0酵母以雜交篩選、加衛工加wn檢測及ip實驗均證明m卜1 」且與功能未知的pit13蛋； 」之間存在著相互作用，說明imj基因除了參與trna剪接之外，還可能具有其它方面的功能，有待進一步研究。 -
Two hybrid protein protein interactions
雙雜交的蛋白質蛋白質相互作用 -
In order to study the upstream and downstream components in g protein and calmodulin signal pathways, a yeast two - hybrid system was used to screen interaction partners of these two proteins
本文利用酵母雙雜交系統分別篩選了與這兩種蛋白相互作用的蛋白質，以進一步研究g蛋白和cam信號轉導途徑中的其它上下游組分。 -
The yeast two - hybrid system is an effective method for the study of protein - protein interaction in recent years. it is widely used in the study of life science because it provides a genetic means of identifying proteins that physically interact in vivo
酵母雙雜交系統是近年來發展起來的一種研究蛋白質?蛋白質相互作用的有效方法，由於它檢測的是一種體內的相互作用，自這一系統建立以來，就被廣泛地應用於生命研究的各個領域。 -
These work have provided us important clues to understanding biological functions of pres and hbv pathogenesis. yeast two - hybrid technique is a contemporary new method for detection of direct protein - protein interactions in a eukaryotic cell
對于hbv前s區病毒吸附蛋白及其相應受體的研究必將為我們深入了解hbv前s區的功能及其致病機制提供重要線索。 -
The results of gst - pulldown and yeast two - hybrid showed kyot2 interact with them respectively in and yeast and vitro. 3 using the yeast two - hybrid system, we isolated a novel protein, kbp ( kyot binding protein ). three types of cdnas were identified by rt - pcr and were designated as kbp1, kbp2, kbp3, respectively
通過rtpcr我們獲得了kbp全長的cdna ，並發現它存在三種不同的剪接體ep 、 kbpz和ep3 ，同時通過計算機比較還獲得了它的基因組全長，這三種cdna和基圇組序列已經在g bank上登錄于邱。 -
The study on the function and mechanism of phrip1 is important for clarifying how the cell plate and cell wall form in plants. in this study, full length of phrip1 is amplified by pcr and ligated into pks plasmid, then the bait plasmid, peg202 - phrip1, is constructed. the inseret gene are sure to be translated into the right fusion protein through its sequence. in the yeast two - hybrid system, the bait plasmid ( peg202 - phrip1 ) and a reporter plasmid ( psh18 - 34 ) are introduced into the yeast ( egy48 ) by co - transformation. then cdna library ( which is in pjg4 - 5 ) is screened and two genes are obtained. the two insert gene fragments are sequenced. one of them is plastocyanin, the other is putative photosystem i reaction center subunit ii precursor, both of them are the necessary components of photosynthetic chain
成膜素相關蛋白1 （ phrip1 ）是一個含608個氨基酸的蛋白質，它對于植物胞質分裂中細胞板的形成起到了十分重要的作用。研究phrip1的功能和機制，對在分子水平上闡明植物細胞板以及細胞壁形成的機理具有重大的生物學意義。在本實驗中，根據phrip1的序列設計引物對其進行pcr擴增，得到該基因后將其連接到了pks質粒上，並進一步構建成了誘餌質粒peg202 - phrip1 。 -
To identify the interaction between kyot2 and zo - 2 - i3, yeast two - hybrid system, purification of kyot2 protein and gst pull - down assay were performed in the experiments. after kyot2 and zo - 2i3 exchanged their vectors, yeast two - hybrid test revealed physical binding of the two proteins
本實驗通過酵母雙雜交及gstpull downas腳驗證了kyotz蛋白與zoz蛋白在體外的相互作用，並通過酵母雙雜交實驗初步確定其相互作用的位點。 -
Using yeast two - hybrid system with lim domain protein kyotl as a bait, we have isolated an alternatively spliced form of human tight junction protein 2 - - - - - - zo - 2 - i3. the tight junction ( or zonula occludens ) is the most apical junction between neighbouring epithelial cells or endothelial cells
53為深入研究kyoth在notch rbpjk途徑中的可能作用及其作用機制，本實驗室以kyoth為誘餌蛋白通過酵母雙雜交得到人類緊密連接蛋白zoe 。 -
Using yeast two - hybrid system honjo ' s group in kyoto university isolated a novel protein, kyot, which physically interacts with rbp - j. differential splicing gives rise to three transcripts of the kyot gene, kyotl, kyot2 and kyots. kyot2, but not kyotl negatively regulates transcription by association with rbp - j
為進一步探討rbp - j的功能，日本京都大學的honjo研究組利用酵母雙雜交系統篩選到一個可以與rbp - j相互作用的新蛋白kyot ， kyot蛋白有三種不同的剪切體： kyot1 ， kyot2和kyot3 ，其中kyot2可以與rbp - j相互作用抑制其轉錄活性。 -
In order to assess the function of rbp - j, a yeast two - hybrid screening using rbp - j as a bait protein was employed and a lim protein, kyot, was isolated
Kyot是一種lim結構域蛋白，有兩種亞型kyoti和kyotz ，在染色體上由同一基因編碼 -
These results suggest that kyot plays an important role in the development of testis and spermatogenesis. 2 we performed yeast two - hybrid screening of a cdna library from human lymph nodes using kyot2 as a bait protein. 42 clones were gotten after 5xl06 were screened by four kinds of nutrition limitation and p - galactosidase assay, 22 clones were got after restriction of positive clones, at last, 13 genes were got by sequence assay including rbp - j known as the interacting protein with kyot before and two novel genes
對此22個克隆進行序列分析，共獲得13種基因，其中包括已知的kyot相互作用蛋白rbpj和兩個未知基因，也包括在哺乳動物睪丸中特異3第四軍醫大學博士學位論文性表達的蛋白piasi ，同時還篩到了具有可與lm結構鞠互作用的pdzdomain分子緊密連接蛋白2和兩種同屬于kg家族的蛋白ringi和polycomb2 -
In order to assess the function of kyot2, northern blot, immunohistochemical sabc and in situ hybridization methods were used to find out the expression of kyot and kyot knockout mice which were kept in our laboratory were analyzed. moreover, we employed a yeast two - hybrid screening using kyot2 as a bait protein and got 13 interacting proteins. the function of a novel protein of them, kbp was investigated
為了進一步研究kyot的功能，我們利用northern印跡、兔疫組織化學等方法對kyot在體內的分佈進行時空定位，並利用本室懈的kyot基困剔除小鼠分析其表型，同時採用酵母雙雜交篩到tkyoth相互作用的13種分子，並對其中的一個新分子kbp進行了功能的研究 -
We find that the fusion proteins are inclusion bodies when the bacteria lysised by the lysozyme. the product of pet - 32a ( + ) - igf - i was analyzed by western blotting, which confirmed that the hybrid protein was expressed as expected
菌體採用溶菌酶裂解，融合蛋白以包涵體形式存在；對higf -融合蛋白進行了westernblot分析，確認所表達蛋白為目的蛋白。
分享友人