hydantoinase 中文意思是什麼

hydantoinase 解釋
乙內酰脲酶
  1. By the technology of gene cloning, bioconversion of d - amino acids with engineered cells containing d - hydantoinase and d - carbamoylase would be expected to overcome the drawbacks presented by using the original strains described above. according to the reported amino acid sequence of d - hydantoinases, two primers were designed and synthesized

    本文根據文獻報道的海因酶基因序列及大腸桿菌對密碼子的偏愛性分別設計了正向及反向引物，以基因組dna為模板，利用pcr技術擴增得到菌株pseudomonasputidayz - 26和sinorhizobiummorelensess - ori的d -海因酶基因。
  2. 3. its shown from the results of dna digestion, hydantoinase gene amplification, rapd, eric - pcr etc, that the electrophoretic patterns of the products are obviously different form the original one

    分別提取的基因組后進行酶切分析、海因酶基因的擴增、 rapd 、 eric - pcr等檢測，兩者均呈現不同的電泳圖譜。
  3. A screening method that allows available, efficient and reliable selection of hydantoinase - positive micro - organisms was devised. the soil samples were collected from different region, which were enriched by using 5 - phenylhydantoin as the unique nitrogen. the first detection was through screening plates, the 23 candidate strains were further selected by detection of the medial product and final product

    2 .建立了一個可行的篩選模型，從不同區域採集土樣，以5一苯基乙內酞服為唯一氮源進行土樣富集，通過平板篩選法進行初篩后，得到23株候選菌株，通過檢測中間產物和終產物的產生進行復篩，結果初步確定了1株乙內酞脈酶產酶菌株，編號為bts11 。
  4. With the procedures, the overall recovery of enzymatic activity reached 20 % and the specific activity for substrate hydantoin was about 4 u / mg protein. the purification factor was about 4. 7 folds with the purity about more than 95 % as estimated by sds - page analysis. d - hydantoinase gene from strain ss - ori was cloned to five different vectors to be five recombinant plasmids and in turn to transfer into five different e. coli strains, respectively

    對其中產酶活性最高的一工程菌pexsec一hdt /雲coljblz ] （ de3 ）海因酶的表達條件進行了研究，目的蛋白的表達量約占總菌蛋白的20 % ，其酶活力為0 . 92u / ml ，約是原始菌株的d一海因酶表觀活性的4 . 6倍。
  5. Hydantoinase ( ec 3. 5. 2 ) hydrolyzes its substrate 5 ' - monosubstantial hydantoin to enantiomerical n - carbamyl - amino acids and in turn, the resulting product can be chemically or enzymatically converted into the corresponding optically active amino acids

    海因酶（ hydantoinase ， ec3 . 5 . 2 ） ，是一類催化海因、 5 』 -單替代海因及其衍生物環酰胺鍵斷裂的酰胺水解酶。
  6. In bioconversion of a d - amino acid and its derivative, the substrate, 5 ' - monosubstituted hydantoin is firstly transferred into an intermediate, d - carbamyl amino acid by d - hydantoinase, and in turn into an optically active d - amino acid by either d - carbamoylase or chemical, nano2. nowadays, the bioconversion of d - amino acids has been industrialized by enzymes, so called one - step process

    D -型氨基酸及其衍生物的生物轉化，經歷兩個反應歷程，首先是在海因酶的作用下，催化5 -單替代海因形成氨甲酰類氨基酸中間物，後者再在酶或化學的作用下，生成相應的光學活性氨基酸或其衍生物。
  7. One strain possessing hydantoinase activity were isolated and named bt811. it was proved that the bt811 was a new d - hydantoinase - producing strain by gene separation and sequence analysis

    后經基因分離和序列分析，證實bt8ll為1株新的d一乙內酞脈酶產酶菌株。
  8. And strain ss - ori is sinorhizobium sp. according to the " blast " data. in addition, a hydantoinase gene ( 1440bp ) from yz - ii6 was amplified by pcr with genomic dna as the template

    我們還利用pcr方法從菌株yz - 6基因組中克隆得到了海因酶的基因（ 1440bp ） ，並構建了表達質粒pet3a - hdtase 。
  9. Two dna fragments encoding d - hydantoinase gene were amplified by pcr from chromosome dna of pseudomonas putida yz - 26 and sinorhizobium morelense ss - ori, respectively, and confirmed by dna sequence analysis

    序列分析表明，來自yz - 26菌的d -海因酶基因全長1440bp ，編碼479氨基酸，基因序列已被genbank登錄（ ay387829 ） ，被確定為一個新基因。
  10. During cloning hydantoinase gene from the original strain ss - ori, we occasionally picked up a colony ( yz - ii6 ) which occurred a high hydantoinase activity. a series of experiments were performed to confirm whether this strain was the same as the original one

    我們在克隆出發菌株ss - ori海因酶基因的過程中，偶然得到了一個能呈海因酶陽性反應的菌落，定名為yz - 6 ，並做了較為細致的研究。
  11. The enzyme is homologous dimmer with molecular mass 102600 as determined by gel filtration on hplc and subunit mass 52042 as determined by maldi - tof mass spectrometry. the optimal ph of d - hydantoinase is at near 9. 5 and the optimal temperature is at 45

    通過對其生化性質的研究表明，其單體m _ r為52042 ，天然m _ r為102600 ，最適ph為ph9 . 5 ，最適溫度為45 ，不同的二價金屬離子對酶活性有不同的影響。
  12. Puc18 - 169 was a subcloned plasmid containing the whole operon sequence of l - hydantoinase from arthrobacter bt801 which can convert 5 - benzylhydantoin to l - phenylalanine. hydantoin hydrolase which is responsible for the ring opening of hydantoin is one of the components of hydantoin utility enzymes of arthrobacter bt801. n - carbamoylase is a part of hydantoinase operon which can transform n - carbamoylamino acids into the corresponding amino acids

    節桿菌（ arthrobacter ） bt801是由軍事醫學科學院生物工程研究所保存的l -乙內酰脲酶產酶菌株， puc18 - 169是由本室構建的含有節桿菌bt801的l -乙內酰脲酶完整操縱子序列的亞克隆質粒。
  13. As far as the enzymatic activity is concerned, on the one hand, strain yz - ii6 has higher d - hydantoinase activity and lower d - carbamoylase activity so as not to be suitable for one - step bioconversion of d - amino acids, on the other hand, the higher hydantoinase activity, engineered strain in particular, may be convenient to be as a biocatalyst to produce n - carbamyl d - amino acids which hard to find in the markets

    Yz - 6菌具有較高的海因酶活性，但n -氨甲酰基d -氨基酸酰氨水解酶活性很低，仍不適合用於d -型氨基酸生產工藝中的一步法轉化。另一方面，含海因酶基因的人工菌株不但酶活性高，也排除了天然菌株轉化生產d -型氨基酸過程中的一些副產物。
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