hyl 中文意思是什麼
hyl
解釋
希爾-
Hyl series cincture type presses filter machine has the heavy treatment ability. with such remarkable characteristic as high efficiency and having long performance life etc, it apply to the environmental improvement of every profession and trade extensively
Hyl型帶式壓濾機具有處理能力大，脫水效率高，使用壽命長等顯著特點，廣泛應用於各行業的環境治理。 -
Thirdly, the hyl gene was cloned to puc19 and pbr322 respectively. then the fragment containing ampr derived from puc19 and hasa came from pse380 - has were inserted into hyl either or both in vitro. transformed the recombinated vectors into s. equi by electro - operation, then plating on hag solid medium containing ampicillin, and selected clones hi which the hyl gene was inactivated by gene replacement through homologous recombination
（ 1 ）直接插入外源基因滅活hyl方法：將hyl片段與載體puc19 pbr322連接后，用來自puc19的amp ~ r抗性基因片段從hyl基因的中部插入將載體上的hyl片段分為兩部分； （ 2 ）用hasa替換hyl部分片段的方法：將hyl片段與載體pucl9 pbr322連接后，用hasa片段替換部分hyl ，再將amp ~ r片段接入到hasa上游。 -
Secondly, the hyaluronate lyase gene ( hyl ) was cloned into the vector puc19 by pcr using the total ona sample ofs. equi as template and partially sequenced, too
Equi的總dna為模板，通過pcr方法，克隆了透明質酸分解酶基因（ hyl ） ，測序后連接到表達載體pse380的trc啟動子下游，構建表達質粒pse380 - hyl 。 -
Finally, we get some transformants with lower hyl activity
分別用4種改造質粒轉化s equi 。 -
Result competence can be induced in vitro and the expressing level of three virulence, lyta, nana, hyl, are higher in wild type than in the come mutant
另外還作了comc的表達測定，從基因水平確定感受態的發生。結果s pn在自然條件下轉化時，培養后吸光度到0 -
The expression vector pse380 - / iy / was constructed and transformed into e. coli dh5a, expressing hyl gene by adding iptg into the broth. the expression of hyl gene showed a 120kda protein band on sds - page gel and was found to have capability to degrade ha molecules derived from a microorganism dissolved in 0. 1 m acetate buffer solution ( ph4. 0 )
經轉化大腸桿菌dh5a和iptg誘導表達後用sds - page電泳分析，獲得一條約120kda的表達條帶； iptg誘導表達后提取原生質膜測定透明質酸分解酶活力，表明該hyl片段的產物能夠在體外分解細菌來源的ha 。採用兩種策略滅活hyl基因。
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