immunoglobulin igg 中文意思是什麼
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The result of elisa displayed that the all the serum against cdv and cav, and immunoglobulin against cav had high activity. but the igg against cdv did n ' t appear a high activity
結果表明狐抗cdv 、 cav高免血清和雞抗cdv 、 cav卵黃的活性都較高,但提純的抗cav的狐igg和雞igy的活性較高,而提純的抗cdv的狐igg和雞igy的效價較低。 -
Test 3 : detected activity of serum and immunoglobulin samples by indirect elisa test, rabbit antibody against foxes ' igg and hen ' s labeled with hrp igy was used in indirect elisa test
試驗三:間接elisa檢測的抗體活性。用過氧化物酶標記的兔抗狐igg和雞igy抗體進行間接elisa測定獲得的樣品的活性。 -
By elisa analysis, inhibition of binding of clq with the c ! q receptors on u937 cells and competitive inhibition of binding of clq with aggregated immunoglobulin g b y selected phage clones, and dna sequencing, a number of similar, but not identical, sets of sequences of clq - binding clones were identified. the deduced amino acid sequences of selected 9 peptides are wyegpftlytwp, hwdpfslsayfp, ltqhnspffllp, tsnpfflwypqp, qtpfqlw, npfnwts, spfxlts, fltwldp and fstflyp. they show significant efficiency to inhibit the binding of clq with the clq receptors on u937 cells and / or aggregated immunoglobulin g, which suggest that the selected peptides contain the modeling epitopes of clq receptor to bind the collage - like region or igg to bind the head domain of clq
然後,採用噬菌體肽庫技術,以c1q為釣餌蛋白,從12肽庫和環7肽庫中親和篩選能與c1q結合的噬菌體克隆,經elisa 、 u937細胞配體結合抑制試驗、 aigg競爭抑制試驗及dna測序,獲得了9個具c1q抑制活性克隆的dna序列,其相應的氨基酸序列為: wyegpftlytwp 、 hwdpfslsayfp 、 ltqhnspffllp 、 tsnpfflwypqp 、 qtpfqlw 、 npfnwts 、 spfxlts 、 fltwldp 、 fstflyp ,它們可能模擬c1qr和或igg的c1q結合表位並抑制c1q的活性。 -
Hybridomas secreting monoclonal antibodies ( mcabs ) specific for clostridium perfringens type a enterotoxin ( cpe ) were produced by fusion of nso myelomas cells with spleen cells from balb / c mice immunized with purified cpe. wells containing hybridomas secreting immunoglobulin g ( igg ) antibodies against cpe were specifically identified by an indirect enzyme - linked immunosorbent assay ( elisa ), and two strains of elisa - positive hybridomas were selected and cloned forth by limited dilution
該純化cpe對小白鼠的半數致死量為2 . 5ug /只;該cpe豚鼠皮膚藍斑單位為2500 4000 ;引起紐西蘭白兔小腸積液的最小毒素量為25ug 。將純化的cpe ,在含0 . 4福爾馬林的的pb液中透析制備類毒素。
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