inclusion body 中文意思是什麼
inclusion body
解釋
【醫學】包涵體。-
After the protein refolding of denaturant inclusion body following dialysis, we got the pure recombinant gst - eo protein by gst affinity columns. using the purified protein as coating antigen, an indirect elisa were developed for detecting the anti - eo antibody in the csfv serum by exploring the concentration of coating an tigen and dilution degree of serum
使用分步透析法對變性的包涵體進行復性，將復性蛋白過gst親和層析柱得到純化的gst - eo融合蛋白。以gst - eo融合蛋白為診斷抗原，初步建立了用間接elisa檢測豬瘟血清eo抗體的方法。 -
The study of the surface - enhanced raman scattering spectra of prgv inclusion body
包涵體表面增強拉曼散射光譜的研究 -
Inclusion body encephalitis
包涵體腦炎 -
Chlamydia inclusion body
衣原體包含體 -
Pathological and immunopathological changes of polymyositis and inclusion body myositis
多發性肌炎及包涵體肌炎的病理和免疫病理變化 -
Several immune system cell types and processes have been recently identified in muscle in inclusion body myositis, dermatomyositis, and polymyositis
近來，包涵體肌炎、皮肌炎、多發性肌炎中幾種免疫細胞類型和免疫反應過程被認定。 -
The analysis of sds - page indicated a fusion protein band at the site of 20 - 30kda appeared in the form of inclusion body
Sds - page分析表明，重組菌在分子量20 - 30kda處出現一高表達蛋白條帶，此誘導表達的蛋白在沉澱中以包涵體形式存在。 -
Myeloid dendritic cells, which contribute to an immunologic synapse responsible for activation of the adaptive immune system, are abundant within muscle in inclusion body myositis and polymyositis
作用於免疫突觸反應、活化適應性免疫應答的髓樣樹突狀細胞在包涵體肌炎和多發性肌炎中大量存在。 -
The recombinant b. thuringiensis subsp. israelensis b - pmpx2 can produce a rhombus crystalline inclusion body during its sporulation sds - page analysis proved that cytlaa had overexpressed during the sporulation of the recombinant. it was found that b - pmpx2 strain remained stable toxicity, whatever during vegetative phase or sporulation phase, which was similar to the expression of mtxl gene in the strain of protease ( s ) - deficient b. sphaericus
同時，將來源於蘇雲金芽孢桿菌以色列亞種（ b . thuringiensissubsp . israelensis ， b . t . i ）的cytlaa基因和p20伴侶蛋白基因克隆連接到質粒pmt9中，並轉化到蘇雲金芽孢桿菌無晶體型中得到重組轉化子b - pmpx2 ，電鏡觀察發現重組轉化子b - pmpx2形成一菱形晶體。 -
The recombination vectors were transformed into host strain of bl21 and induced with iptg. all the recombinant protein was expressed into inclusion body. the recombinant protein was identified with sds - page and westen - blot and purified through elution method. the muticlone antibody was got by immuning the rabbit with the purified protein
把重組質粒轉化入表達受體菌bl21 ，經iptg誘導后都獲得了表達，且都以包涵體的表達形式存在，用sds - page和western - blot的方法對重組蛋白進行了鑒定。 -
This article presents a systematic pathology study of inclusion body hepatitis of avian mnong 32 million chickens of 87 chicken farms during the period of 1994 - 1995 in the central south section of hebei province
介紹了河北省中南部地區1994 - 1995年間， 87個雞場， 3200萬只雞發生雞包涵體肝炎的系統病理學研究。 -
The reults of sds - page and western blot dermonstrated that the insoulable component of the induced e. coli culture contained protein 3a. the results of the study indicated that protein 3a existed in the form of inclusion body. the content of the expressed protein in the induced bacteria protein was 29. 2 % and 22. 1 % respectively
Sds - page和westernbolt結果證實，大腸桿菌菌體不可溶性蛋白中富含3a蛋白，且此融合蛋白的分子量符合預期設計，說明3a蛋白在表達產物中以包涵體的形式存在，所表達的蛋白含量分別占菌體蛋白的29 . 2和22 . 1 。 -
The more favorable experiment conditions of preparing anatase nanometer tio2 powder are obtained from a lot of data. preparation technology of rutile nanometer tio2 powder is researched on the base of experiment of anatase nanometer tio2 powder. the influences of enclosure dose ' s quantity, preroasting temperatures phase - transition accelerant ' s quantity and calcining intensity and so on on the properties of inclusion body - zntio3 / ti ( oh ) 4, granule size and properties of rutile nanometer tio2 powder are discussed
在銳鈦型納米tio _ 2粉體的制備基礎之上，進一步研究了金紅石型納米tio _ 2粉體的制備工藝，探討了包覆劑用量、預焙解溫度、晶型促進劑量及焙燒溫度和保溫時間等因素對znco _ 3 / ti （ oh ） _ 4沉澱包覆體性能、金紅石型納米tio _ 2粉體產品的粒度和性能的影響。 -
The expressed fusion protein occupied more than 20 % of total bacterial protein. the fusion protein induced mainly existed in the insoluble inclusion body of the cell, only little parts of fusion protein expressed in the cytoplasm, excreted into periplasm and secreted into the cultural medium as soluble protein. through ultrasonic treatment at low temperature, soluble expressed fusion protein could be obtained from the supernatant of lysed cell
表達產物在細胞內外的精細定位研究表明，融合蛋白cbd - lt 27在經誘導的大腸桿菌中表達量占總菌體蛋白的20以上，融合蛋白主要以不溶性包涵體的形式存在於細胞中，少量以可溶產物的形式存在於細胞質、分泌于細胞周質間腔及培養基中。 -
4. the expressed result of pbv - a, pbv - b and pbv - c showed that the expressed protein was soluble and no inclusion body was been found. sds - page analysis show the molecular weight of protein expressed by phaa was 42kda which was equal to 3 - ketohilase, the molecular weight of protein expressed by phab was 26kda which was equal to acetoacetyl - coa reductase, and the molecular weight of protein expressed by phac was 63kda which was equal to pha synthase
表達載體pbv - a 、 pbv - b和pbv - c經誘導表達，發現所表達的蛋白均為可溶性蛋白，沒有包涵體出現；蛋白經sds ? page分析表明，基因phaa表達的蛋白分子量為42kda ，與?酮硫裂解酶分子量大小一致；基因phab表達的蛋白分子量為26kda ，與乙酰乙酰coa還原酶分子量大小一致；基因phac表達的蛋白分子量為63kda ，與pha合成酶分子量大小一致。 -
Conclusion : it ' s possible to improve the expression efficiency by adjusting the g + c content of tir region and codon usage. replacement the cystein 78 and cystein 96 into serines, higher bioactivity gained compared with control hbfgf protein, and decreased amout of dimer and inclusion body, this suggested that free thiol group of cys78 and cys96 is the main reason of dimer and multimer formation
結論：通過調整tir區g c含量及增加大腸桿菌偏愛的密碼子，確實能提高hbfgf在大腸桿菌中的表達量，而將78和96位上的半瞇氨酸替換成絲氨酸后，得到的hbfgf的蛋白活性高於未改構蛋白，二聚體和包涵體減少。 -
The high titer specific ndrg2 antibody is indispensable to reseach deeply the functions or the tissue and subcellular distribution features of ndrg2, ha order to prepare ndrg2 antibody, the whole ndrgl sequence was cloned into prset - a vector and two truncated sequences of ndrg2 were cloned into pgex - 4t - l vector. after induced by iptg, the fusion proteins were expressed in e. coli ; rabbits immunized with the whole length ndrg2 protein were reinforced with two shortened fragments of ndrg2 ; after immunization, rabbits produced high titer antiserum against ndrg2. then antisemm was absorbed using ndrg2 antigen immobilized on nc filters, the purified product of antiserum shows high special to ndrg2 protein, and the separated inclusion body of 6his - ndrg2 will be useful for the further reseach
為制備高效價的ndrgz抗體，分別構建了prset a雌、 pgex4t d唾倉和pgex4tl七三種原核重組表達質粒，並在大腸桿菌中誘導表達出相應的融合蛋白；用全長gstjqdrgz蛋白免疫兔，然後用gst ndrgz人和gstjqdrgze片段加強免疫，經免疫得到了較高效價的兔抗人ndrz多克隆抗血清，利用固定於硝酸纖維素膜上的ndrgz抗原親和吸附純化抗血清，提高了ndrgz抗體的特異性；並對包涵體形式表達的6his ndrgz進行初步的分離純化。 -
With the development of dna recombination technology, there are also some reports about the expression of recombined ifn protein by prokaryotic expression system, but the expressed proteins often consist in the form of inclusion body = the operations about extracting and purifying proteins are very complicated, biological activity of proteins are not high
隨著dna重組技術的發展，國內外亦有利用原核表達系統表達重組ifn蛋白的報道，但表達的目的蛋白常以包涵體形式存在，提取純化操作步驟復雜、蛋白活性不高。 -
By gene fusion and prokaryotic expression, we purified a pea actin isoform ( peac1 ), his - tagged peac1, his - tagged gfp and his - tagged peac1 - gfp from inclusion body. after filtrating a series of induction condition, we expressed and purified his - tagged peac1 with soluble form in a large amount
利用基因融合技術，原核表達並從包涵體中純化了豌豆肌動蛋白異型體peac1 、 his - taggedpeac1 、 his - taggedgfp以及his - taggedpeac1 - gfp ，並通過誘導條件的篩選達到了可溶性表達與大量純化his - taggedpeac1 - gfp的目的。 -
We used gradient dilution renaluration ( renaturation buffer : 0. 14mol / l tris - hcl ph8. 0. 10mmol / lgsh, 2mmol / lgssg. 5 % glycerol, 0. 02mol / l l - arg ) and gel filter renaturation by sephacral s200 to refold the inclusion body respectively. to get pure nk4, it was purified by gst affinity gromatochraphy. to detect the angiostatic activity of nk4 in vivo, it was tested using cam
6 ）誘導溫度對pg廠x一4t一l一nk4表達菌株bl21表達目的蛋自的影響分別進行了25 』 c 、 300c 、 37溫度狀態卜，以iptg化學誘導，發現其均可表達，全菌表達量: 37最高，其次為30誘導時。
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