inside-out patch 中文意思是什麼
inside-out patch
解釋
膜內面向外式-
The patch clamp experiment : the cell - attached and inside - out configurations of the patch - clamp techniques were used to investigate single channel properties of nmd a receptors in the actuely dissociated neurons of the marginal division in the rat striatum, recording the current amplitude. long / short term opening / closing times indexes and the effects of incitants and agonists of the nmd a receptors on those parameters. we find that two types of conductance of 42ps and 20ps were recorded, the 42ps was the advantaged conductance
本研究是在本實驗室以前研究的基礎上，通過膜片鉗（ pachclamp ）觀測sd （ sprague - dawley ， sd ）的mrd上nmda受體通道的電生理特性；繼而對青壯年sd大鼠的mrd 、海馬進行毀損並腹腔注射nmda受體阻滯劑ktm （ ketamine ， ktm ） ，觀察其對大鼠學習記憶能力的影響：對于學習記憶能力下降的老年鼠進一步做顱內定位注射nmda受體激動劑與抑制劑，觀察其在特定部位的作用；最後，應用fmri （ functionalmagneticresonanceimaging ; fmri ）對注射ktm的志願者做學習記憶與痛覺的對比觀察。 -
In this experiment, the inside - out configurations of patch - clamp techniques were used to study the physiological properties, phosphorylation modulation and temperature sensitivity of bkca channel in hypothalamic neurons of newborn sd rats. objective : studing the physiological properties. phosphorylation modulation and temperature sensitivity of calcium - activated potassium channels ( bkca ) in hypothalamic neurons of newborn sd rats in order to elucidate the role of bicca channel in temperature regulation
本實驗研究了sd乳鼠下丘腦神經元大電導鈣激活鉀通道（ large - conductanceca ~ （ 2 + ） - activatedpotassiumchannels ， bk _ （ ca ） ）特性的溫度依賴性及磷酸化對其溫度依賴性的影響，以求為體溫調節機制的最終闡明提供重要的理論依據。 -
Activities of bkca channels were studied at the single - channel level with inside - out patch - clamp configuration. bkca channels in neurons after reoxygenation for 6 h showed a significant increase in the unitary conductance and open probability compared with control. after reoxygenation for 24 h, both the conductance and the open probability recovered to the control level
採用膜片鉗內面向外式單通道記錄方法研究發現，復氧6小時神經元bkc ：通道電導及開放概率與正常對照組相比均顯著增加，復氧24小時通道電導及開放概率恢復至正常水平。
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