insoluble protein 中文意思是什麼
insoluble protein
解釋
不溶蛋白-
Thrombin is an enzyme that catalyzes the conversion of the soluble plasma protein fibrinogen to the insoluble protein fibrin.
凝血酶能催化可溶性血漿纖維蛋白轉化為不溶性的纖維蛋白。 -
Globulin a kind of globular protein that dissolves in dilute salt solutions but is relatively insoluble in pure water
球蛋白：是一種可溶於稀鹽，但在純水中相對難溶的球狀蛋白。 -
In the young cheese, all nitrogen is present in the form of insoluble protein.
在未成熟的乾酪中，全部氮都以不溶性蛋白質狀態存在。 -
Its content was about 9. 8 % among total cell protein by gene genius bio imaging system. the fusion proteins were found largely in an insoluble inclusion bodies. the purified fusion proteins was obtained by his6 technique used to immunize rabbits to obtain polyclonal antiserum with titer of 1 * 105
經工ptg誘導，重組質粒在點co力『 blzi中表達出了c端融合了6xhis的融合蛋白，過量表達的蛋白主要以不溶性蛋白形式存在，其表達量占菌體總蛋白的9 . 8 % 。 -
A tough, insoluble protein substance that is the chief structural constituent of hair, nails, horns, and hoofs
角蛋白一種不溶解的較硬的蛋白物質，是頭發、指甲、角和蹄的主要結構成分 -
The expressed fusion protein occupied more than 20 % of total bacterial protein. the fusion protein induced mainly existed in the insoluble inclusion body of the cell, only little parts of fusion protein expressed in the cytoplasm, excreted into periplasm and secreted into the cultural medium as soluble protein. through ultrasonic treatment at low temperature, soluble expressed fusion protein could be obtained from the supernatant of lysed cell
表達產物在細胞內外的精細定位研究表明，融合蛋白cbd - lt 27在經誘導的大腸桿菌中表達量占總菌體蛋白的20以上，融合蛋白主要以不溶性包涵體的形式存在於細胞中，少量以可溶產物的形式存在於細胞質、分泌于細胞周質間腔及培養基中。 -
The coding region of cdna was cloned into procaryotic expression vector pet30a and overexpressed in e. coli bl21 ( de3 ). the cyclase proteins extracted from bacterial culture were found largely in the insoluble protein fraction
Cdna編碼區序列被克隆進原核表達載體pet - 30a ，並在大腸桿菌bl21 （ de3 ）中誘導表達，但過量表達的蛋白主要是以不溶性蛋白形式存在。 -
Objectives : in our previous study, to improve the expression efficiency of human basic fibroblast growth factor ( hbfgf ) expressed in escherichia coli, we lowered the g + c content in hbfgf ' s tir region and used more preferred codons by e. coli. meanwhile we found the low insoluble product with a large quantity of dimer and higher multimer in the protein solution. it greatly decrease the bioactivity of hbfgf
目的：我們在長期實踐中發現，大腸桿菌中表達的人堿性成纖維細胞生長因子（ humanbasicfibroblastgrowthfactor ， hbfgf ）在e . coli中表達量不高，可溶性低，蛋白溶液中大量存在二聚體，三聚體以至多聚體，使得hbfgf的生物活性大大降低。 -
Though the expression level of bl21 ( de3 ) / pt221 - hyuh was lower than that of m15 / pqe60 - hyuh, the target protein of bl21 ( de3 ) / pt221 - hyuh was principally in soluble form while the objective protein of m15 / pqe60 - hyuh was principally in insoluble form. both of the products in the two strains showed biological activity, but the former is 2 times higher than the latter. the hyuc dna fragment was inserted into ppic3. 5k plasmid to construct the ppic3. 5k - hyuc recombinant plasmid which was then transduced into pichia pastoris gs115 cells after being linearized by bgl ii digestion
結果表明， sds - page分析在50kd處有一較強的表達帶，融合有分子伴侶的重組菌株bl21 （ de3 ） pt221 - hyuh與非融合表達的重組菌株m15 pqe60 - hyuh相比，乙內酰脲水解酶的表達量低一些，但其表達蛋白主要以可溶性形式存在，而m15 pqe60 - hyuh中表達蛋白則主要以包含體形式存在，且前者的酶活性是後者的2倍多。 -
The expression protein products as insoluble inclusion bodies accounted for 15 % and 30 % of whole bacterial protein. two methods, b - per reagent only and triton - urine with sonication lysis of cells, were both access to the relatively good washing effect
通過摸索iptg誘導濃度，誘導溫度，菌體收獲時間等條件，確定了eo基因能在pgex4t bl21 （ de3 ） codonplus和pmal - p2x bl21 （ de3 ） codonplus中高效表達，表達產量分別占菌體總蛋白的15和30 ，表達的重組蛋白主要為包涵體。 -
Unfortunately, the fusion protein was insoluble, either
不幸的是，融合蛋白仍以包含體形式存在。
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