karyoplast 中文意思是什麼
karyoplast
解釋
核體,核原生質體-
Traditionally, somatic nuclear transfer in mammal is completed by micromanipulation. but micromanipulation is evidently damaging to cytoplast cytoskelton, causing the lost of some important factors involved in programming of donor karyoplast, reducing the volume of cytoplasm
傳統核移植總效率低，且需要熟練的顯微操作技術，所需設備昂貴，限制了核移植的廣泛應用；因此作為替代，化學去核應運而生。 -
Since the success of dolly, the first cloned sheep with the adult somatic cells as karyoplast donor, new approaches have been developed for nuclear transfer technology. here we describe a handmade cloning method which combines the chemical induced enuleation and zona - free technology in embryo culture. enuleated oocytes were derived by exposing the oocytes to demecolcine and cytoheximide supplemented mdium sequently and its chromosome was depleted to the first polar body
將培養10h的化學去核卵母細胞與供體成纖維細胞融合后lh 、 2h 、 3h ，分別有77 . 6 % 、 70 . 6 % 、 58 . 9 %重構胚的染色質發生凝集，其餘胚胎的染色體則處于原核期;而只在融合后3h ， 27 . 9 %重構胚被標記出組裝的紡錘體，且其中的同源染色體己經分離。
分享友人