lacz 中文意思是什麼

lacz 解釋
拉茨
  1. By using primers designated for lacz gene, pcr result suggested an integration of lacz into u. pinnatifida genome. nine reagents including penicillin g, kanamycin, g418, teicoplanin, zeocin, chloramphinicol, hygromycin, basta and methotrexate were tested as selective reagents for selection of transgenic sporophytes. the results showed that young sporophytes of u. pinnatifida were sensitive to chloramphinicol and hygromycin and very sensitive to basta which suggest the potential of using the resistance genes as selectable markers

    ，為了解決轉基因裙帶菜的篩選問題，進行了裙帶菜幼孢子體對不同篩選壓力的敏感性實驗，包括抗生素類的青霉素g 、卡那黴素、 g418 、硫酸鏈黴素、鹽酸潔黴素、 zeocin 、氯黴素、潮黴素，除草劑類的basta ，氨甲喋呤類的氨甲喋呤，結果顯示裙帶菜幼孢子體對氯黴素、潮黴素敏感，對basta非常敏感。
  2. Fixed with gluteraldehyde, stained with ph 7. 4 phosphate buffered x - gal dye solution, observed by reverse microscope, the results are as follow : the percent of larvae expressing lacz in midgut showed that fb - 28 improved the sensitivity of larvae to virus

    病毒感染后的8h ， 12h ， 16h ， 20h提取中腸組織，按常規石蠟包埋切片進行洗滌、透明、浸蠟、包埋、切片、粘片等操作程序，製成連續切片，在倒置顯微鏡下逐一進行觀察計數。
  3. Sodium succinate as carbon source could lead to earlier sporulation and earlier expression of cryld - lacz, when sodium acetate acted as carbon source, cryid - lacz could keep high, stable expression in different hosts

    琥珀酸鈉作碳源時導致芽胞形成提前， cry1d - lacz融合基因表達也隨之提前。而以乙酸鈉作碳源時，在不同亞種的菌株中都能高水平、持續穩定地表達。
  4. In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv

    本研究採用脂質體轉染方法，將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞，經6輪蝕斑克隆、篩選、表達， pcr鑒定和dot - elisa檢測，證明該重組病毒已構建成功，並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。
  5. Vp3 gene of hl isolate of goose parvovirus derived from recombinant piasmid pproex htb - vp3 was subcloned into ecorl site of psy538, and the reporter gene lacz with promoter pll was cloned into smai site of recombinant piasmid. both vp3 gene and lacz gene were cloned into noti site of psy681, recombinan fpv transfer vector containing vp3 gene of gpv was obtained. the result is basis of construction of recombinant fpv expressing vp3 gene of gpv and gpv genetic engineering vaccine

    本研究另從含有gpvh1分離株主要結構蛋白vp3基因的重組質粒pproexhtb - vp3中切取gpvh1株vp3基因片段，將其亞克隆于psy538的ecori位點，然後將帶有痘苗病毒啟動子p11的lacz報告基因平端克隆于上述重組子的smai位點，再用noti切下同時含有vp3基因和lacz報告基因的片段，再亞克隆于psy681的noti位點，構建出含有vp3基因的重組禽痘病毒轉移載體，為構建表達vp3基因的重組禽痘病毒從而制備gpv基因工程疫苗奠定了基礎。
  6. The recombinant vector was digested with tthllll and the lacz gene from e. coli was inseted in this site, the generated plasmid is designated as pltk - uni

    然後定向亞克隆swha基因於多克隆位點，獲得重組轉移載體pltk - ha 。
  7. ( 4 ) in order to investigate the influence of the carbon source on the expression of cryld - lacz, cryld - lacz was introduced into different strains and cultured in liquid medium containing different carbon sources

    檢測了在蘇雲金芽胞桿菌不同菌株中，不同碳源對cry1d - lacz融合基因表達的影響。
  8. The percent of larvae expressing lacz gradually increased for various time points after inoculation, but the percent of larvae expressing lacz reduced after 20 hours post inoculation, we thought the infection spots transformed

    、從卜又』蕎!鄉;三、卜率卻有所降低，對此，我們分析其原因可能是由於病毒的感染部位發生了轉移。
  9. The transient expression of lacz driven by crtw 306bp s ' - flanking sequence and crtz 302bp s ' - flanking sequence shows that these two sequences habour transcription regulatory sequences

    以lacz為報告基因的瞬間表達實驗結果表明，長度分別為306bp和302bp的crtw和crtz5 '上游側翼序列具有很強的啟動轉錄活性，提示兩段序列包含了啟動子的結構。
  10. ( 2 ) influence of the promoter and upstream region on expression of cry id was tested. firstly, the pcr products of cry id promoter and its upstream were obtained followed by the construction of shuttle plasmid containing cryld - lacz

    盡管cry1dmrna比cry1abmrna的半衰期更長，但cry1d和cry1ab轉錄時間和轉錄量的差異是導致其表達量差異的重要原因。
  11. When cotransforming pgbd - nifa and ad fusion genomic library into saccharomyces cerevisiae pj69 - 4a, 109 candidates interacting with nifa had been selected by testing for the expression of the his3, ade2 and lacz reporter genes

    誘餌質粒和文庫質粒共轉化釀酒酵母（ saccharomycescerevisiae ） pj69 - 4a ，通過檢測報告基因his3 、 ade2及lacz的表達進行篩選，初篩得到109個陽性酵母菌落。
  12. Analysis of activity of p - galactosidase showed that cryld - lacz expressed differently in the same host because of different carbon sources and differenly in different hosts utlizing same carbon sources, suggesting that metabolism pathway of carbon source in hd - 133 - 5 was special

    首次報道了cry1d - lacz在同一菌株中的表達因碳源不同而異，在碳源相同的條件下不同菌株的表達也不相同，而衍生菌株hd - 133 - 5的代謝途徑非常特殊。
  13. Several vegetative clones of male or female gametophytes of u. pinnatifida has been induced and parthenogenesis of female gametophytes has been observed. 90 % of the gametophytes can grow into young sporophytes one month after fertilization. no staining background of either lacz or gus was found in different tissues of u. pinnatifida

    進行裙帶菜配子體孤雌生殖、孤雄生殖和雌雄受精的再生途徑實驗，結果沒有得到孤雄生殖的裙帶菜，孤雌生殖的效率低、時間長且發育不正常，雌雄受精的再生途徑一個月左右90 %以上的雌配子體都正常萌發成幼孢子體。
  14. When compared with kds301 ( lexa + ), xe ( lexa - ) cells showed quite similar levels of survival curve to y - irradiation and mmc, indicating lexa gene had no effect on radiation resistance of deinococcus radiodurans. deinococcus radiodurans " lexa had no revelance with its extremely radioresistance. the recombinant plasmid pza172 was constructed by cloning the lexa gene of deinococcus radiodurans into the vector plasmid puc19 in the downstream of lacz promoter

    對lexa基因突變體xe的研究表明，其輻射抗性與lexa基因野生型的抗輻射菌kd8301相近，存活曲線上均顯示有一個寬大的肩區，結果發現lexa基因的突變並沒有改變抗輻射菌的獨特抗性，說明lexa基因與輻射抗性無關。
  15. Finally, a tranfer vector named as pltk - ha was constructed based on pltk - uni with insertion of ha gene of h3 subtype srv. the pltk - ha and prv bartha - k61 genomic dna were co - transfected into 50 - 70 % confluent vero cells in 6 - cm dishes. based on the expression of lacz gene, recombinant prv were selected and purified by blue - colored virus plaque

    利用脂質體介導的方法，將pltk - ha與prvbartha - k61基因組共轉染于亞單層vero細胞，依據報告基因lacz在細胞中的表達，篩選藍色蝕斑的重組病毒，經數次蝕斑純化后， pcr鑒定、 western - blot分析。
  16. After obvious cytopathogenic effects developed, virus - contained supematants were harvested, and the progeny viruses were screened for lacz - expressing viruses by a plaque assay using x - gal. single blue plaques were picked, and a recombinant prv stably expressing lacz gene ( designated as rprv - lacz ) was obtained after ten cycles of plague purification and pcr identification. the results showed that the lacz gene expression cassette was stably expressed in the recombinant rprv - lacz derived from bartha - k61 strain

    該載體與具有高度感染性的bartha - k61株基因組dna通過脂質體加plus法共轉染vero細胞，採用甲基纖維素固定病變， x - gal染色，經過10代藍斑純化獲得了一株穩定表達lacz基因的ge tk基因缺失突變株，命名為rprv - lacz 。
  17. In this study, a 1. 7kb kpni fragment and a lacz gene expression cassette carrying the e. coli lacz gene under the control of sv40 promoter were inserted into the transfer vector pbdtk - uni ( a 277bp acci - accl fragment in the tk gene was deleted ). the new transfer vector was called puni - lacz. the transfer vector puni - lacz and purified genomic dna of strain bartha - k61 were used to cotransfect vero cells using lipofectin transfection procedure

    本研究以呈ge ~ -表型的經典弱毒疫苗bartha - k61株為親本株，在通用prv轉移載體pbdtk - uni的基礎上，在其多克隆位點中插入由sv40早期啟動子控制下的lacz基因表達盒，同時將下游同源臂增加了一個1 . 7kb的kpni片段，使上下游同源臂的長度都超過了1kb ，構建了一個新的轉移載體puni - lacz 。
  18. Suggesting that the larvae of spodoptera exigua died more quickly when fb - 28 were added to the virus. the larvae of spodoptera exigua inoculated as newly molted fourth instars ( 40s ) and at various hours after molting ( 316s ), we sacrificed larvae at various time intervals after inoculation to process the larvae for lacz expression in midgut by using tissue section techniques on early phase infection

    此外，對于處于不同蟲齡的幼蟲進行病毒感染時發現：對3 ~ （ 16 ）幼蟲毒力生物測定時，熒光增白劑的增效作用達到50 ，但對4齡初的供試幼蟲進行測定，熒光增白劑的增效作用卻不很明顯，僅為10 。
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