level of escherichia coli 中文意思是什麼
level of escherichia coli
解釋
大腸桿菌含量- level : n 1 水平儀，水準儀；水準測量。2 水平線，水平面；水平狀態；平面，平地。3 水平，水準；水位；標準；...
- of : OF ＝Old French 古法語。
- escherichia : n. 【微生物學】埃希氏菌屬。
- coli : 大腸菌的
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Fusion protein expression system can overcome those problem, increased the yield of yield of recombinant protein in e. coli. this remarkable increase in protein yield was thought to be due to protection of the target protein from proteolysis, improved folding, and efficient mrna translation. fusion protein also make the detection and purification easy, is a good strategies for achieving high - level expression of genes in escherichia coli
小分子量異源蛋自在人腸桿菌的表達受mrna不穩定、翻譯起始效率低、易被蛋自酶降解等因素的干擾，較難獲得高效表達，通過與已高表達的蛋自融合表達可以克服以上問題，可以使大多數蛋白獲得高效表達。 -
High - level expression of bydv gav coat protein gene in escherichia coli
株系外殼蛋白基因在大腸桿菌中的高效表達 -
High level expression of pngase f in escherichia coli and its bioactivities
在大腸桿菌中的高效表達及其脫糖基化作用研究 -
The plasmids pci - mbl54 containing full length of mutations mbl cdna were propagated hi escherichia coli xl - 1 blue, then the extracted and purified pci - mbl54 were used to transfect dhfr ( - ) chinese - hamster ovary ( cho ) cells. after screeened with g418 and cloned, 4 g418 - resistent clones were randomly selected for detection of mrna expression by rt - pcr and molecular beacons. it was found that all of the 4 positive cell clones expresse mbl analogue as detected in transcription level
抽提、鑒定、純化重組質粒后，脂質體轉染法將重組質粒導入中國倉鼠卵巢細胞（ cho - dhfr ~ - ）中， g418選擇轉染子並克隆化培養，經rt - pcr和分子燈塔探針雜交鑒定其mrna轉錄，獲得4株穩定表達54位密碼突變型mbl的cho細胞。 -
After washing with reagent, adopt the newest purification technology source30rpc, sds - page and densitometric scan analysis, the result show that expression level is 90 % of total bacterial proteins. after renaturation, ifnr, hgfa, hgfb, hpk5 were purified by akta purifier chromatogram instrument, sepharose fast flow, ssphacrayl series gel, selecting optimize condition. finally establish a kind of high efficient purification model of recombinant proteins produced in escherichia coli as inclusion bodies, purification product purity > 98 %
結論:總之，通過對發酵罐中重組工程菌各種培養因素的研究，建立了一種高密度、高表達發酵工藝體系，為重組蛋白的后續純化提供了大量、穩定的原料供應;通過對不同目的蛋白的色譜行為的系統研究，建立了一種高效穩定、快速簡潔、易於放大的包涵體重組蛋白分離純化體系。
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