line of segregation 中文意思是什麼
line of segregation
解釋
偏析帶- line : n 1 線；繩索；釣絲；測深度用繩，捲尺。 a fishing line 釣魚線。 be clever with rod and line 會釣魚...
- of : OF ＝Old French 古法語。
- segregation : n. 1. 分離；分開；隔離。2. 【化學】分凝；【物、冶】偏析；熔析；【生物學】分異。 【機械】離析性。3. （等位基因）分離。n. -ist （種族）隔離主義者。
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The characteristics of tm - 22 expression presented in transgenic tobacco : 1 ). virus specificity in either homozygote or heterozygote ; 2 ) tm - 22 gene integrated in tobacco genomic dna with single copy and in inheritance and segregation to progenies on the mendel role ; 3 ). transgenic line with tm - 22 promoter ( ptm47 ) showed infected symptoms with cell death distinguished to one with 35s promoter ( ptm49 ) after inoculation with tomv - 2a
其次，通過氨基酸序列和結構的比較，確定tm - 2 ~ 2基因的編碼蛋白與tomv病毒在抗病反應中相互識別的特異氨基酸及其功能；然後，應用重組dna技術，互換tm - 2 ~ 2基因和tm - 2基因的對應結構域，構建嵌合基因，獲得嵌合蛋白表達的轉化體，驗證tm - 2 ~ 2編碼蛋白中變異氨基酸的作用。 -
Methods : ( 1 ) the segregation of foreign target gene in the t1 by histochmical gus assays ; ( 2 ) identification of pure line from transgenic tomatoes ( tl ) through examining gus expression in pollens in conjunction with pcr analysis of marker gene ( npt ) ; ( 3 ) the transcript levels of leetrl or leetr2 in anti - sense transgenic plants ; ( 4 ) the phenotypes of the transgenic plants in tomato during whole life cycle under ethylene - treated and non - treated conditions
本研究以反義乙烯受體leetr1 ， leetr2基因番茄t _ 0代種子為實驗材料，利用gus基因表達研究外源基因的遺傳規律，並藉助于pcr技術對目的和標記基因的鑒定獲得轉基因t _ 1代材料。利用gus基因在t1花粉中的表達鑒定獲得轉基因純合植株。研究了轉基因後代的生長發育模式、對外源乙烯敏感性，以及靶基因的表達特性，初步探明了它們在乙烯受體系統中的功能。 -
These results demonstrated that merely examining the segregation of reporter gene in pollens is a reliable method that could avoid the laborious testing process of the pure line from transformed plants ; ( 3 ) the transcript levels of leetrl and leetrl were checked by northern blotting analysis
（ 3 ）兩個基因在反義植株里的表達都受到不同程度的抑制。在反義leetr1植株里， leetr1表達被抑制后， leetr2的表達也受到不同程度的抑制。同時，反義leetr2植株里也有相似的結果。
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