liposome entrapment 中文意思是什麼
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Different amount of copies in different tissues attribute to the different density of positive signals. the result of the experiment suggested that the transgenic animals can be produced by spermatozoa - mediated gene transfer after the entrapment of liposome. and because the exogenous dna occurs losing the segments. partly integration, or existin g outside of genome dna, the rate of chimerism is relatively high
結果表明: ( 1 )脂質體包裹外源基因轉染精子的方法,可將外源基因導入受精卵中,能夠獲得轉基因動物,並得到了較高的轉基因陽性率; ( 2 )精子攜帶的外源dna的整合過程是隨機的,在受精過程和胚胎早期分化過程中可能發生了片段丟失、不完全整合或游離于基因組存在而產生嵌合體。 -
The plasmid plncxhlf which is combining with 2366bp human lactoferritin ( hlf ) cdna complete sequence and 800bp regulating sequence upsteam by the - lactoglobulin gene promoter is prepared by normal steps. then it is used in the following experiments. the recombinant plasmids plncxhlf were introduced into mouse mammary tumor cell line ma - 782 by liposome entrapment
本研究將含有人乳鐵蛋白( hlf )基因2366bpcdna序列完整序列和800bp的奶山羊-乳球蛋白基因5 』端調控序列的乳腺特異表達重組載體plncxhlf按常規方法提取、純化、 pcr 、酶切鑒定后,用於細胞表達和精子介導轉基因動物的研究。 -
Abstract : 5 - fu liposome was prepared by the reverse evaporating method. then the entrapment reached 43. 31 % by freeze - thawing 5 - fu liposome several times. the centrifugal accelaration experiment and the measurement of potential proved that the stability of the liposome could be ameliorated
文摘:採用反相蒸發法制備5 -氟尿嘧啶脂質體制劑.結合凍融法,可使5 -氟尿嘧啶的包封率達( 45 . 31 0 . 2085 ) % .離心加速實驗和電位的測定進一步驗證了凍融法可使脂質體穩定性提高
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