multiplex pcr 中文意思是什麼
multiplex pcr
解釋
多重pcr-
Rapid detection of dengue viruses by multiplex pcr assay
技術快速鑒定登革病毒 -
The tailed primer design protocol is feasible for y - str loci and can be used to develop more multiplex pcr assays of y - str loci. the results of validation prove that the y - a489 - plex multiplex pcr system can be accepted for the forensic dna typing
證明y一a489一plex ，適合於法醫dna分析，有較高的應用價值，為法醫y染色體分型提供了新的技術手段。 -
The purposes of our work are to establish a simplified method of multiplex pcr based on chimeric primers for str loci, to develop a set of fluorescent quadriplex str system for forensic dna typing based on this method, and to validate the forensic application of the system under the guidelines of tmgdam ( the technology working group on dna analysis methods ) in order to address concerns presented in today ' s legal environment
目的本課題旨在探索一種新的str基因座復合擴增方法，我們稱為嵌合引物str復合擴增法。應用熒光標記毛細管電泳激光自動檢測技術平臺，建立一套新的法醫str基因座復合擴增體系，並按照美國dna分析方法技術工作組（ thetechnologyworkinggroupondnaanalysismethods ， twgdam ）的指導方案進行法醫學實用性研究。 -
Rapid detection of enterohemorrhagic escherichia coli in food with multiplex pcr assay
快速檢測食品中腸出血性大腸桿菌 -
A series of validation experiments and genetic studies should be performed for the y - str multiplex system according to the suggestion of the technical work group dna analysis methods ( twgdam ). method we selected four y - str loci, dys434, dys438, dys439, a10 ( y - gata - a10 ) and designed two set of tailed primers to improve the efficiency of the multiplex pcr
方法選定四個y染色體str基因座，應用加尾序列引物設計策略設計的引物，構建四個基因座的y - str復合擴增體系，建立銀染檢測和熒光檢測方法，依據dna分析技術工作組（ twgdam ）指南進行法醫學可行性研究和群體遺傳研究。 -
Result. a method of multiplex pcr based on both chimeric and universal primers was established. a fluorescent quadriplex str system, including d1s1612, d9s2026, d20s161 and d6s477, was developed on the basis of the multiplex pcr
建立了dis1612 、 d952026 、 d205161 、 d65477str基因座熒光標一記嵌合引物復合擴增毛細管電泳激光自動分析體系，同時證明嵌合引物復合擴增方法與毛細管電泳激光自動分析方法相兼容。 -
The use of chimeric primers provides a method for primer design that eliminates the multiple optimization steps involved in developing multiplex pcr. our fluorescent quadriplex str system can be analyzed by capillary electrophoresis and provide a new sources of typing regent for this technique platform with laser detecting system
結論建立了法醫四個遺傳標記dis1612 、 d952026 、 d205161 、 d65477新的熒光標記嵌合引物復合擴增體系，證明該體系可成功應用於熒光標記毛細管電泳激光自動分析檢測平臺。 -
Rapid detection of listeria monocytogenes in food with multiplex - nested pcr
檢測食品中單增李斯特菌研究 -
The y - a489 - plex multiplex pcr is feasible for using home product of taq dna polymerase
Y一a489一plex體系採用的是國產的taq酶。 -
Result a multiplex pcr system of four y - str loci ( y - a489 - plex ) was established. here, y - a489 means the selected loci dys434, dys438, dys439, a10
在100個樣本中檢出37個單倍型，單倍型變異度為0 . 9628 ，標準誤為0 . 00429 。 -
Furthermore, the home product of taq dna polymerase had the same specificity and efficiency compared to the amplitaq gold ( pe, usa ) in this study. conclusion this is the first time that the tailed primer design protocol for multiplex pcr system is used for y - str loci
初步構建了四個y一str基因座的y一a489一plex銀染體系和y一a4sg一plex熒光體系:並依據dna分析技術工作組（ twgdam ）指南進行了應用性研究。 -
Detection of strawberry mottle virus and strawberry mild yellow edge virus by multiplex rt - pcr
檢測草莓斑駁病毒和草莓輕型黃邊病毒 -
Detection of lily symptomless virus and lily mottle virus by multiplex rt - pcr
檢測百合無癥病毒和百合斑駁病毒 -
Also, these str loci were amplified by multiplex pcr using our primer set, but the universal primers were fluorescent - labeled. the products of the multiplex pcr were genotyped by abi prism 310 genetic analyzer
結果通過採用公共引物和基因座特異性嵌合引物的方法，建立了法醫str基因座嵌合引物復合擴增方法。 -
Research of genetically modified rapeseed detection with multiplex pcr - gene chip
晶元聯用檢測方法的研究 -
In order to make y - str markers more widely accepted, population studies and robust assays are required. we focus on developing new multiplex pcr for y - str loci that can be detected by sliver staining system and fluorescent system
本課題旨在通過研究復合擴增技術，構建四個y - str基因座的復合擴增銀染檢測體系和復合擴增熒光檢測體系，並對其進行法醫學可行性研究和群體遺傳研究。 -
For multiplex pcr, primers specific for z and y a, which are involved in the dsb, were employed. and also, a pair of primers of act1 was used as a negative control. in this experiment, we also used yku70 as a positive control, since it has been well defined as a dsb end binding protein
雖然這一發現只是我們工作的一個開端，但是基於這一開端，兩個層面的研究工作將被很大程度地拓寬，一是泛素系統不僅只是在dna修復的prr路徑起作用，而是可能涉及dna修復的各種不同路徑。
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