pcr 中文意思是什麼

pcr 解釋
PCR ＝polymerase chain reaction 【生物化學】聚合酶聯鎖反應〈此項技術可用以復制犯罪現場發現的DNA小樣，從而有助於破案〉。

  1. Application of pcr to identifying suspicious abo blood group

    疑難血型鑒定中的應用
  2. By means of discontinuous tris - hcl buffer system and poly aery 1 amide gel electrophoresis ( page ), zymogram of 23 species of 11 genera in 4 subfamilies mirinae, orthotylinae, phylinae and deraeocorinae are gained. by specific primer pcr and sequencing, 57 cyt b gene 433bp sequences are obtained from 34 species respectively belonging to 17 genera in 5 subfamilies mirinae, orthotylinae, phylinae, deraeocorinae, bryocorinae from 64 species of 23 genera which involved in this research, and 2 outgroup species of family anthocoridae as well. 1

    首次通過特異引物擴增和基因測序的研究方法從被試的5亞科23個屬的64種盲蝽中獲得了盲蝽亞科、合墊盲蝽亞科、葉盲蝽亞科、赤爪盲蝽亞科和蕨盲蝽亞科bryocorinae5個亞科17個屬的34種盲蝽以及外群花蝽科anthocoridae2種花蝽的cytb基因序列57條。
  3. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  4. Application of real - time quantitative pcr for guidance therapy of cytomegalovirus infection after allo - hematopoietic stem cell transplantation

    造血幹細胞移植后對人巨細胞病毒感染病毒載量的檢測
  5. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  6. ( 2 ) at translation level plant mutual sequence of starting translation aaca was added to start codon of t - pa gene by pcr ampliation and plant expression vector pbet was constructed

    （ 2 ）在翻譯水平上通過pcr擴增的方式在t - pa基因起始密碼子處添加了植物翻譯起始共有序列aaca ，構建了植物表達載體pbet 。
  7. The pcr product was inserted into expression plasmid pet - 32a ( + ) after restriction digest. then the recombinant plasmid was identified by endonuclease analysis, pcr ampliation and dna sequencing. the report showed that the recombinant plasmid had right open reading frame

    重組質粒經酶切鑒定， pcr鑒定和測序，結果證實豬肺炎支原體黏附因子p97基因的抗原決定簇r1區定向插入了質粒pet - 32a （ + ） ，且閱讀框架正確。
  8. Equipment and instruments : electronic analytic balance, uv and vis spectrophotometer, 97rt fluorescence spectrophotometer, gas chromatograph spectrometer, high speed centrifugal machines, leica rm 2015 microtome, fluorescence microscopes, pcr amplifier, and so on

    :電子分析天平、紫外可見光分光光度計、 97rt熒光分光光度計、氣相色譜儀（附4種檢測器） 、高速離心機、病理切片機、熒光顯微鏡、 pcr擴增儀等。
  9. Considering of the specificity of the degenerative primer designed in this pcr reaction, the identity between the sequence we wanted and the fragment of pcr product and the presence of asmaspa, asmaspb, clr and cls ( the homologous gene of masp gene ) in halocynthia roretzi, a japanese ascidian, we believe that the sequence of pcr product is some part of the masp gene or masp homologous gene

    基於本實驗中所設計的引物為特異性簡並引物，測序基因通過比較得到與預期片段有一定的同源性以及masp同源物asmaspa 、 asmaspb 、 clr和cls在海鞘中存在的事實，我們可以初步推測，本實驗pcr反應所克隆的片段可能為文昌魚masp或其同源基因的一部分序列。
  10. It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity

    本研究中，木耳屬2個種的2個菌株在its區域表現出較高的保守性， 4種限制型內切酶的酶切圖譜沒有顯示出多態性；增加內切酶種類及供試菌株數量，有可能獲得具有多態性的限制性內切酶酶切圖譜； 9本實驗中， its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異，擴增片段長度均為650bp左右； 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析，紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法，兩者的關系是相輔相成，互為驗證
  11. The results are as following : 1 eric - pcr was for the first time applied in differentiating strains from edible fungi and proved to be more rapid and reliable than rapd in auricularia identification study. taken the similarity coefficient as 75 %, 29 strains of three auricularia species were grouped into 6 and 9 clusters by rapd and eric, respectively. eric - pcr clearly distinguished a. auricula from a. polytricha while rapd failed

    在75的分類水平上， eric - pcr把29個菌株分為9組，黑木耳種和毛木耳種可以明顯區分開；而rapd只能分為6組，不能將黑木耳和毛木耳分開，說明eric - pcr是比rapd更快捷可靠的分子標記，可以有效用於木耳屬的種質資源及遺傳分類的研究，也適合於其它食藥用菌種質資源的研究； 2
  12. More and more fluorescent signal can be collected with the pcr reaction carry on. the method is more automatized and much less time consumption ( only 3 hours from nucleotide hybridization capture to result found )

    這樣利用熒光信號積累可以實時監測整個pcr進程，實現了pcr擴增和核酸雜交以及熒光電信號放大檢測同步進行的自動化檢測技術；實時熒光pcr技術具有更大的優越性： l ）不需要pcr后處理，
  13. Pcr detection of prawn white spot bacilliform virus using molecular beacon probe

    在流感病毒檢測上的應用
  14. By using primers designated for lacz gene, pcr result suggested an integration of lacz into u. pinnatifida genome. nine reagents including penicillin g, kanamycin, g418, teicoplanin, zeocin, chloramphinicol, hygromycin, basta and methotrexate were tested as selective reagents for selection of transgenic sporophytes. the results showed that young sporophytes of u. pinnatifida were sensitive to chloramphinicol and hygromycin and very sensitive to basta which suggest the potential of using the resistance genes as selectable markers

    ，為了解決轉基因裙帶菜的篩選問題，進行了裙帶菜幼孢子體對不同篩選壓力的敏感性實驗，包括抗生素類的青霉素g 、卡那黴素、 g418 、硫酸鏈黴素、鹽酸潔黴素、 zeocin 、氯黴素、潮黴素，除草劑類的basta ，氨甲喋呤類的氨甲喋呤，結果顯示裙帶菜幼孢子體對氯黴素、潮黴素敏感，對basta非常敏感。
  15. In the experiment, the full code sequence of bar gene was cloned by pcr from transgenic herbicide resistant bobwhite wheat and checked. it was expressed in e. coli and its protein was determined. after having been properly modified, the bar gene which correctly codes pat was cloned into binary vector pbi121 and then transferred into lba4404 by triparantal crossing, which is the prerequisite work for genetic transformation

    本實驗從抗除草劑轉基因bobwhite小麥中，利用pcr克隆的方法擴增出bar基因全長，並在原核表達系統中表達，鑒定表達蛋白的活性，將能夠正確編碼ppt乙酰轉移酶的bar基因片段，經過適當的修飾構建入真核表達載體。
  16. Transformed shoots were selected on solidified medium containing kanamycin. ten kanamycin resistant transformants were obtained by direct or induction calla. these transformants were checked by pcr, pcr - southern blot and southern blot, confirmed that two positive transformants were integrated into the genome of boechmeria nivea l guad

    對所得到的抗性轉化株進行了pcr 、 pcr - southernblot 、 southernblot檢測，其中2株呈陽性，證明vp4基因已整合到苎麻基因組dna中。
  17. According to the phylogenetic tree, the thirteen strains were grouped into four distinct pcr - rflp clusters, namely, coriaria group, myrica group, myrica - casuarina - alnus group and casuarinarmyrica group

    結果顯示弗蘭克氏菌種群內的遺傳多樣性較高，種群分化較大， 13株供試frankia菌株平均每個位點的多樣性指數為0 . 4498 。
  18. The changing tendencies of the relative contents of phosphorous contained substances have been detected by in - vivo " p magnetic resonance spectroscopy ( in - vivo " p mrs ) during the whole hatching process. in - vivo ] p mrs proved the catabolism of adenosine 5 ' - triphosphate ( atp ), phosphorous ester and phosphocreatine ( pcr ) when the embryo dead. the results could be used to deduce the conversion of phosphorous contained metabolites during the chicken embryo developed

    用活體核磁共振定域氫譜（ in - vivohmagneticresonancespectroscopy ， in - vivohmrs ）對胚胎發育過程中羊水和蛋白、蛋黃的成分進行了分析；用活體磷譜（ in - vivo 』 』 pmrs ）的方法分析了在整個胚胎發育過程中含磷代謝物的相對含量隨時間的變化，表明了磷脂類物質及三磷酸腺苷（ atp ） 、磷酸肌酸（ pcr ）在此過程中的變化及可能的相互轉化的趨勢，胚胎死亡后的磷譜也證明了磷脂類物質及三磷酸腺苷（ atp ） 、磷酸肌酸（ pcr ）在死亡過程中降解為無機磷的現象。
  19. Finally 4 right clones was obtained from 864 transformants by pcr detection. sequencing analysis showed that the hsa gene have been introduced into the right position of the human a - lactalbumin yac. furthermore, works to optimize the conditions of introducing the recombinant yac into goat f ibroblast via cell fusion was explorded

    經pcr檢測，從864個轉化子中獲得了4個陽性克隆，測序表明，人血清白蛋白基因已正確的導入到人-乳白蛋白基因yac的特定位點上，並獲得了可進行細胞融合的重組yac載體。
  20. The open reading frame of tsarg2 was obtained from human testis cdna library by pcr. using in situ hybridization on tissue section of human testis. we preliminarily studied the expression and function of tsarg2

    從小鼠睪丸cdv文庫中分離出該基因完整閱讀框cdna ， ； hu基因的cdna全長為1088hp ，包含6個外顯于，基因組跨越9
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