mutant protein 中文意思是什麼
mutant protein
解釋
突變蛋白質-
The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。 -
This group of mutant alleles turned out to be a gene encoding a golgi adenosine 3 ' - phosphate 5 ' - phosphsulfate transporter ( adenosine paps transporter ). kyte - doolittle hydrophilicity plots showed that sll is a very hydrophobic protein, especially in c terminus
Kyte - doolittle疏水性分析結果表明，與其它轉運子相似， sll蛋白是一個疏水性很強的蛋白（尤其在它的c端） 。 -
Someone tried this way but the yield is low. francis expressed truncated mutant pokeweed antiviral protein gene in pichia pastoris
目前已經有人在這方面做出了嘗試，但蛋白表達產量還不高。 -
Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115. mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting
本研究將含有n末端信號肽和c末端毒性區缺失的pap基因的表達載體ppic9k - p用sali酶切線性化后，通過電擊轉化整合p . pastorisgs115菌株細胞中。 -
Whole cell c2d2 reduction by all four mutants comparing to wild type and ni / v mutant was also detected. the result showed that only single a - gln194 substitution did not perturb the stereospecificity of protonation of c2d2. the above comparing results indicate that in mofe protein ( 1 ) a a - gln190 site and its association with homocitrate are important for the transfer of electron / proton to femoco, while a - his194 site and the homocitrate are independent in h2 evolution
對四個突變株細胞的c _ 2d _ 2還原特性及還原產物中反式-順式- 1 ， 2 -二氘代乙炔的比例進行了測定並與野生型及nifv突變株相比較，結果表明只有- gln ~ （ 194 ）替換不影響c _ 2d _ 2還原產物中反式-順式- 1 ， 2 -二氘代乙炔的比例，即未改變固氮酶還原c _ 2h _ 2加氫的立體構型的專一性。 -
The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku
將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體，然後導入畢赤酵母（ p8chianastoris ）菌株gslls細胞中，在甲醇的誘導下，經過酵母高密度發酵進行pap的表達，經sds page分析，結果表明，在培養基上清液中含有一明顯的特異性蛋臼條帶，大小為34ku ，經western blotting分析，該蛋白與法國pap抗血清有特異性反應，體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性，說明該pap基因在畢赤酵母gs中也得到了正確表達。 -
The page revealed the culture supernatant of the initial strain and the mutant contained different protein bands, which exactly demonstrated at protein level that a. niger j 506 was surely a mutant of a. niger m1. zymogram stained with xylan - remazol brilliant blue for detecting xylanase showed there are three different xylanases in the mutant culture, while two xylanases in initial strain. what is important, the third xylanase in a. niger j 506 have higher activity and more production levels from page and zymogram of xylanase
尤其是在木聚糖酶譜帶檢測中發現，突變株發酵液中有三種類型的木聚糖酶，而出發菌株中只有兩種類型的木聚糖酶，並且通過考馬斯亮藍g250染色和瓊脂糖板上的透明圈發現，突變株中第三種類型的木聚糖酶不僅表達量很大，活力也很高。 -
The results showed that the open reading frame of chil - 15 cdna encompassed 564 base pairs ( bp ) and encoded a protein of 187 amino acids with three potential n - linked glycosylation sites, four conserved cysteine residues, two out - of - frame atg initiation codons in the 5 " untranslated region, and a signal peptide consisting of 66 amino acids. when it was compared with the published sequence of chil - 15 cdna, 7 mutant sites were found, and 5 amino acids were changed in predicted amino acids, which indicated that chil - 15 may be polymorphic
結果顯示，本研究所用白來航雞il - 15cdna5 』非編碼區有兩個框外atg起始密碼子，開放閱讀框由564bp組成，編碼187個氨基酸，其中n末端信號肽含有66個氨基酸殘基，在第48 、 149和166位的天冬酰胺殘基上有三個潛在的n -糖基化位點。 -
Biotechnology companies spend years figuring out the right combination of mutant host organism and reactor conditions to fashion a protein therapy that is safe and effective
生物科技公司耗費數年時間，試著找出適當的突變宿主生物與反應環境組合，以得到安全有效的蛋白質治療方法。 -
The ie 180 mutant combined with two special protein binding - sites and inhibited the promoter transcription. accidentally in the approach showed that the plasimd pcdna3 show as activator to sv40 and cmv early promoter. the result is acquired by instantaneous transinfect
這說明不同的ie180突變體，對于sv40啟動于這類在轉錄起始位置一側只有一個「 5 』 atcgt 3 』 」特徵蛋白質結合序列的11類基因啟動于，可分別表現出抑制活性與激活性。 -
The mutant protein of huntington ' s attacks the railroad system of brain cells and impairs transport of essential materials required for neurons to function
突變的亨廷頓蛋白攻擊腦內細胞的轉運系統，繼而損害了神經元要發揮正常功能所必需的物質的轉運。 -
The pharmacology analysis on the increased current in the single mutant ( sos3 / athkt1 ) indicated that the increased current, possibly contributed by athktl activity, could be inhibited by ttx and ca2 +. it is insensitive to tea * and have the same selectivity to li +. the data suggest that the protein athktl perhaps is a na + - permeable channel
結果顯示athkt1蛋白介導的電流增大可被ttx和ca ~ （ 2 + ）部分抑制，對k ~ +通道抑制劑tea ~ +不敏感，而，盯甫大月卜二幾月目睜困月周口創七掩呀，匕月目卜位倫大且對li +有相似的選擇性，表明athkti蛋白可能為一種na +通透型陽離子通道，其蛋白活性受到5053蛋白的抑制調節。 -
9 expression of mxmybl gene in e. coli was experimented. the structure of its recombinant expression vectors had not base mutant and drift. sds - page of pet - mxmybl proteins showed that there was a strengthened protein band in expectant 38kda protein marker
構建的原核表達載體結構正確，未出現堿基突變及移碼現象； lmmoffeiptg誘導zh后，在預期的蛋白分子量約38kda處出現一條表達加強的蛋白帶。 -
A bt - e. coli shuttle vector pht315 was deleted its replication region of bt, then constructed a novel vector named pht315 - 1 which composed a multiple cloning site, erythromycin and ampicillin - resistance marker and could only replicated in e. coli. used pht315 - 1, a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324. sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501, 333, 183aas. orfl had 98 % identities with replicating related protein ori43 of bt strain hd263. the others were no homology to any published bt replicating related protein. after continuous cultured for 70h at 30 c without antibiotic selecting press. the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 %. and growth curve also showed that the novel replicon was stable and could replicate normally
進一步序列分析表明該復制區至少有3個較大的orf ，分別編碼501 ， 333 ， 183個氨基酸。其中orf1蛋白序列與hd263復制蛋白ori43的同源性為98 ，而另外兩個orf和genbank己公布的bt復制相關蛋白無同源性。 30連續培養72h ，復制區質粒在bt無晶體突變株hd73cry ~ -中穩定性達98以上， 30h生長曲線也表明該復制區能夠在bt中穩定復制和遺傳，對受體菌株無明顯不良影響。 -
Nutrient value protein of mutant j3 of agarious blazei by irradiation
3中蛋白質的營養評價 -
In addition, the researchers have shown that mice lacking the normal form of huntingtin have very similar neurological symptoms to mice that express the mutant form of the protein
此外，研究人員還發現，缺少了正常形式杭丁頓蛋白的小鼠，與表現變異形式杭丁頓蛋白的小鼠相比，具有類似的神經癥狀。 -
Western - blot result indicated that the protein could bind to anti - br antibody specifically. thus, a br mutant ( tyr79 - ? rg ) was successively conducted. the mutant protein was also analysesed by some bioinfomnatic software
通過觀察各種br的突變蛋白在結構和光學性能上的變化，從而確定br結構和功能上的關鍵氨基酸，使我們更深入得了解蛋白質結構和功能之間的關系。 -
The nature of the mutant alleles is either nonsense mutation or the mutation which disrupted the splicing of the primary transcripts. anti - en antibody staining and in situ hybridization on sll germline clone ( glc ) embryo using wg antisense probe showed defective en protein bands and defective wg transcripts bands
通過對sll生殖系克隆胚胎進行的抗engrailed （ en ）抗體染色和wg反義探針的原位雜交結果表明，與野生型果蠅的胚胎相比較， en蛋白和wg轉錄物條帶出現了明顯的缺失。 -
This group of mutant alleles turned out to be a gene encoding a xylosyltransferase, which is an enzyme transfers the xylose from udp - xylose to the serine of the protein core
這一組突變體等位基因編碼一種木糖轉移酶- oxt ，這種轉移酶能夠將udp -木糖中的木糖轉移到核。 -
The mutant protein were found to possess higher bioactivity than native standard protein
檢測改構后之hbfgf蛋白活性高於未改構蛋白。
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