mutant site 中文意思是什麼
mutant site
解釋
突變位點-
Whole cell c2d2 reduction by all four mutants comparing to wild type and ni / v mutant was also detected. the result showed that only single a - gln194 substitution did not perturb the stereospecificity of protonation of c2d2. the above comparing results indicate that in mofe protein ( 1 ) a a - gln190 site and its association with homocitrate are important for the transfer of electron / proton to femoco, while a - his194 site and the homocitrate are independent in h2 evolution
對四個突變株細胞的c _ 2d _ 2還原特性及還原產物中反式-順式- 1 ， 2 -二氘代乙炔的比例進行了測定並與野生型及nifv突變株相比較，結果表明只有- gln ~ （ 194 ）替換不影響c _ 2d _ 2還原產物中反式-順式- 1 ， 2 -二氘代乙炔的比例，即未改變固氮酶還原c _ 2h _ 2加氫的立體構型的專一性。 -
The mature region cdna of melittin of apis cerana was cloned by using puct - vector developed from pucis vector. the sequence was compared with the corresponding region of melittin of apis mellifera reported in genbank, the mutant site was found in 33rd base ( a : apis mellifera ; g : apis cerana ), but the primary structure of the two proteins were identical, which suggested melittin, as an important defensive gene, was very conservative in evolution
測序結果顯示與意蜂（ apismellifera ） melittin成熟區序列相比，中蜂（ apiscerana ） melittin成熟區序列在第33位存在差異（意蜂為a ，中蜂為g ） ，但兩者蛋白質一級結構相同，說明melittin作為蜜蜂一種重要的防禦基因在進化上保守。 -
Pllz1112 was transformed into m145 to disrupt sc6a9. 34. a mutant named hxy1 was obtained and confirmed to be disrupted at correct site by southern blotting
將phz1112導入野生型菌株m145中獲得基因中斷菌株hxy1並通過southern雜交驗證hxy1在正確位置發生了基因中斷。 -
A bt - e. coli shuttle vector pht315 was deleted its replication region of bt, then constructed a novel vector named pht315 - 1 which composed a multiple cloning site, erythromycin and ampicillin - resistance marker and could only replicated in e. coli. used pht315 - 1, a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324. sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501, 333, 183aas. orfl had 98 % identities with replicating related protein ori43 of bt strain hd263. the others were no homology to any published bt replicating related protein. after continuous cultured for 70h at 30 c without antibiotic selecting press. the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 %. and growth curve also showed that the novel replicon was stable and could replicate normally
進一步序列分析表明該復制區至少有3個較大的orf ，分別編碼501 ， 333 ， 183個氨基酸。其中orf1蛋白序列與hd263復制蛋白ori43的同源性為98 ，而另外兩個orf和genbank己公布的bt復制相關蛋白無同源性。 30連續培養72h ，復制區質粒在bt無晶體突變株hd73cry ~ -中穩定性達98以上， 30h生長曲線也表明該復制區能夠在bt中穩定復制和遺傳，對受體菌株無明顯不良影響。 -
It is show the mutant consists of a nuclei binding site and dna - binding domain is enough to negative regulation. a report plasmid pcat that we constructed has a cat gene expressed by a cmv early promoter
（ 465 1079氨基酸段）對sv40啟動子明顯的抑制活性，因為它只包含dna結合域和核結合位點，說明發揮轉錄抑制功能的關鍵是ie180的dna結合域。 -
To construct eukaryotic expression vector of mbl gene with codon 54 point mutation, the target sequence in pgem - mbld plasmid, which conains mbl cdna with codon 54 mutant allele, was amplified by pcr. after the cdna fragement and plasmids pci - neo were prepared by digestion with sma i and sal i, the fragment was inserted into sma i and sal i site in pci - neo eukaryotic expression vector, and the recombinant vector, named pci - mbl54, was obtained. the pci - mbl54, digested with restriction enzymes, was found to contain the point mutation mbl cdna by agarose gel electrophoresis analysis
本實驗以ggc54gacmbl突變為研究對象，選用真核表達質粒pci - neo ，根據已構建好的含54位密碼子突變型mbl基因t載體的結構，設計合成新的引物， pcr擴增54位密碼突變型mbl基因，凝膠回收，雙酶切pcr產物和pci - neo質粒， t4連接酶連接，將前者克隆至後者的sma和sal位點，轉化大腸桿菌xl - 1blue ，氨芐選擇培養。 -
Cat ( chloramphenical acetyl transferase ) elisa assays to identified the function of constructed plasmid. the result indicates mutant p1p2, p1p3p2 and p6p2 repressed to cmv promoter. same as ie promoter two special sequences " atcgt " located at two side of the transcription initial site of promoter
共轉染hplp細胞，結果表明所有的缺失突變體對cmv啟動子都表現抑制活性，我們發現在ie180和p啟動子的轉錄起始位置兩側同時具有兩個特徵序列（ 5 』 atffit 3 』 ） 。 -
I ) two mutants ( hpab - 38 and hpab - 34 ) were designed based on the three - dimension structure of hpab - constructed by protein homology modeling method, ii ) the mutant molecules were generated by pcr and inserted into pfast hta donor plasmid, the later was then transformed into escherichia coli dhlobac to generate recombinant baculoviruses dna by site - specific transposition of an expression cassette into a baculovirus shuttle vector ( bacmid ) propagated in e. coli. the recombinant bacmids were isolated and transfected into insect sf - 9 cells to reproduce baculoviruses
4 . h隊b一p及其突變體高效表達工程菌的構建:將用pcr擴增的h隊b一p 、 h獄b一p35 、 h隊b一p34基因，分別與pinpointxa一3質粒連接，轉化jm109大腸埃希菌，獲得的工程菌在表達融合蛋白時不穩定，第5代后即見不到表達條帶。
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