n-terminal fusion 中文意思是什麼
n-terminal fusion
解釋
n端融合-
Both the mature genes of gloshedobin and gussurobin were cloned into the vector pet - 32a ( + ), strain bl21 ( de3 ), to study their expression in prokaryotic cell. the gene was expressed under t7 promoter with a fusion protein partner of thx. tag and a 6x his. tag at its n - terminal. having been induced by iptg for 4 hours, the recombinant enzyme was examined in the cytoplasm by sds - page analysis
將大連蛇島蝮蛇和長白山白眉蝮蛇毒類凝血酶基因分別克隆到大腸桿菌表達載體pet - 32 ( a ) +中,在t7啟動子下表達出融合蛋白,融合部分為硫氧還蛋白,位於類凝血酶基因上游,並在其n端帶6xhistag標簽以利於表達產物的分離純化,經熱激轉化至宿主菌bl21 ( de3 )中, iptg誘導斗小時后收獲菌體。 -
A human lymphotoxin deletion gene fragment which lacking n - terminal 27 amino acid residues of the protein was pcr amplified using a pair of designed primers and cloned into expression vector pet36b ( + ) to construct a fusion protein with cbd tag, resulting a recombinant plasmid pet36b - lt 27. the recombinant plasmid was transformed into host e. coli bl21 ( de3 ) plyss
採用pcr的方法擴增出人淋巴毒素n端缺失27個氨基酸的缺失體基因片段,將此基因片段克隆至表達載體pet36b ( + )上,與t7lac啟動子控制下的cbdtag構建成表達融合蛋白的重組質粒pe736b - lt 27 。 -
There are so many molecules involved the fertility, such as : spl7, a mammalian testis - and sperm - specific protein, can specifically bind with zona pellucida, dextran, dextran sulfated, and fucoidin. another germ cell cyritestin is a testis - and sperm - specific protein belonging to the adam family. it has a disintegrin domain at the n - terminal, known as a integrin ligand, that suggests cyritestin could be involved in sperm adhesion and fusion with the egg membrane previous study demonstration
配子中的許多分子均參與了上述關鍵環節,如在精母細胞、精細胞和睪丸精子中表達,並特異性與卵子透明帶糖蛋白、葡聚糖、糖酐脂結合的精子表面蛋白17 ( sp17 ) ,以及參與識別或與卵子膜上的整合素相互作用的adam家族成員之一的cyritestin蛋白等。 -
Effect of n - terminal fragment fusion protein of polycystin - 1 on collagen synthesis and degradation in rat glomerular mesangial cells
多囊蛋白1氨基段融合蛋白對腎小球系膜細胞細胞外基質及其降解基因表達的影響
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