n-terminal 中文意思是什麼

n-terminal 解釋
n末端
  • n : 1. 【羅馬數字】90〈N＝90000〉。2. 【化學】＝nitrogen. 3. ＝North（ern）。N ＝nuclear 核的：N-waste 核廢料。
  • terminal : adj 1 終端的，終點的，結尾的；極限的。2 定期的，每期[季]的；每學期的，學期終[末]的。3 期終的；末...
  1. Cloning and identification of chicken and pig bpi protein n - terminal fragment

    氨基端的基因克隆和鑒定
  2. Homologues of era have been identified in prokaryotes and eukaryotes. there are two domains in era : the n - terminal gtp - binding domain and c - terminal rna binding kh domain. era plays an important role in cell cycle progression at a specific point in the cycle, after chromosome partitioning but before cytokinesis

    Era是在原核和真核生物以及植物中普遍存在的g蛋白，是一個結構上既具有gtp結合結構域又有rna結合結構域、不同於ras的獨特的新的g蛋白亞家族。
  3. Prokaryotic expression and characterization of n - terminal truncated glycoprotein gp85 of epstein - barr virus

    端片段的原核表達與初步鑒定
  4. It showed excellent anti - tmv activity. and the n - terminal 19 amino acid residues are " dinfslagadgqtyn tfia " ( accession number p83206 in swiss - prot )

    測得其n -端19個氨基酸序列： dinfslagadgqtyntfia ，與其它植物rip的同源性為10 73 ，與葫蘆科rip的同源性為37 73 。
  5. The experimental method includes selecting pure complexes of histidine - containing or cysteine - containing materials, from c - and n - terminal group of these amino acids to link to a group which have color or fluorescence or ultraviolet absorption, elucidating their binding affinity, fluorescence or uv - visible spectrum properties with zinc at physiological concentration and to elucidate their structure in the solid state via infrared spectroscopy. with the help of the concerned the data, the analysis was done to prove whether it can be applied to the zinc detection, in other words, whether it can be used as a new fluorescence probe for zinc detection

    本實驗首次選用在生物體內與zn ~ （ 2 + ）鍵合能力很突出的物質? ?組氨酸和半胱氨酸，採用類似於多肽合成的方法，在其羧基或氨基分別嫁接上一個帶有標記的基團，生成穩定的共價鍵化合物；在此化合物中模擬生理濃度條件加入鋅離子，通過紅外圖譜、紫外圖譜或熒光圖譜的變化分析鋅離子對標記基團是否產生影響，再結合有關數據分析其是否適合檢測鋅離子，即是否可能作為新的鋅離子熒光探針。
  6. The extracellular 100pl ( ecl1 ) and ecl2 was linked by disulfide bond to maintenanced the stability of the protein secondary structure. in recent years, we showed that ccr5 as a co - receptor could interact with hiv - 1 infect ion. ccr5 was paid closed attention to since it was cloned in 1996. the aim is to - obtain the sequence of first extracillular domain of 3 chemokine receptor 5 ( ccr5 ) n - terminal gene fragment with high level expression in e. coli and to prepare its specific antibody f ( ab ' ) ;, and its detected method

    Ccr5具有g蛋白偶聯受體家族（ g - proteincoupledreceptors ， gpcrs ）所特有的7個跨膜區（ transmembrinedomains ， tm ） ，呈螺旋， tm的氨基酸有很高的保守性，膜外第一襻（ extracellularloop1 ， ecl1 ）和ecl2之間有二硫鍵相連，以維持蛋白質二級結構的穩定性。
  7. We fused the gfp into the c - terminal region as well as n - terminal region of emt - 1 protein. the result showed that the localization of the protein encoded by the full length emt - 1 cdna was in endoplastic reticulum ( er ). extracellular region, transmembrane and intracellular region showed the similar cellular localization

    我們用綠色熒光蛋白融合於emt l全長及其不同截斷形式的梭基和氨基端，瞬時轉染cos 7細胞，通過熒光顯微鏡觀察，發現emt l編碼的蛋白呈現內質網定位的特點。
  8. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  9. An unusual rice calmodulin isoform, oscam61, was first obtained in our lab, which contains an n - terminal cam domain and a c - terminal basic extension with a potential prenylation site. in vitro activity assays confirm oscam61 as a functional calmodulin. using the green fluorescent protein ( gfp ) as a visual marker, we further studied subcellular localization of oscam61 in stably transformed tobacco cells

    利用綠色熒光蛋白（ greenfluorescentprotein ， gfp ）作為標記，研究了oscam61在煙草細胞中的定位， gfp - oscam61融合蛋白（具有開放的異戊烯化修飾位點）定位於細胞質膜和細胞器膜上，而oscam61 - gfp （異戊烯化修飾位點被gfp封閉）定位於細胞核的核質中。
  10. It is shown that the obtained proteins ( hq - 5 & tsk - 2 ) can be identified as novel components of anti - coagulation enzyme family in the venom of agkistrodon acutus by the analysis of the n - terminal sequence of tsk - 2

    其一級序列為rtefqrymeivv 。與從蘄蛇蛇毒中分離出的其它組分相比， hq - 5及其降解產生的組分（ tsk - 2 ）為新的抗凝血組分。
  11. Both the mature genes of gloshedobin and gussurobin were cloned into the vector pet - 32a ( + ), strain bl21 ( de3 ), to study their expression in prokaryotic cell. the gene was expressed under t7 promoter with a fusion protein partner of thx. tag and a 6x his. tag at its n - terminal. having been induced by iptg for 4 hours, the recombinant enzyme was examined in the cytoplasm by sds - page analysis

    將大連蛇島蝮蛇和長白山白眉蝮蛇毒類凝血酶基因分別克隆到大腸桿菌表達載體pet - 32 （ a ） +中，在t7啟動子下表達出融合蛋白，融合部分為硫氧還蛋白，位於類凝血酶基因上游，並在其n端帶6xhistag標簽以利於表達產物的分離純化，經熱激轉化至宿主菌bl21 （ de3 ）中， iptg誘導斗小時后收獲菌體。
  12. 57 protein spots out of about 1000 detectable spots on the 2 - d gels were indentified by the following two methods : l ) n ~ terminal edman degradation microsequencing after the protein spots were electro - transferred to pvdf membrane. 2 ) maldi - tof - ms peptide fingerprint analysis of the protein spots and protein database searching. the 2d protein maps of the rice spikelet during the sterile and fertile stages were compared

    二是通過原位酶解，抽提蛋白質片段進行maldi - tof質譜分析，利用肽質量指紋圖數據在數據庫中進行檢索，在雙向電泳凝膠上取了57個點進行分析，有34個蛋白質點在數據庫得到歸屬鑒定。
  13. The pocket accommodating the aromatic ring of phenylalanine is formed predominantly by hydrophobic side - chains, including those of ile10, ile13, prol50, leul75, leul79, phe209, ser211 and val221 ; whilst the amino group and carboxylate group of phenylalanine is coordinated by asp6, asp7, gln151 and ser180. the n - terminal region is found to be important to the function of arog. point mutation reveals that residue substitution of ilelo to ala10 leads to desensitization in the presence of 1 mm phenylalanine, simultaneously, exhibits a dramatic decrease in enzymatic activity

    綜合前人的研究和本研究工作的實驗數據，得出以下結論： 1 ）在arog的反饋抑制位點供苯丙氨酸結合的疏水口袋由asp6 、 asp7 、 ile10 、 ile13 、 pro150 、 gln151 、 leu175 、 leu179 、 ser180 、 phe209 、 ser211和val221等12個氨基酸殘基構成； 2 ）苯丙氨酸結合的疏水口袋分為兩個區域，與苯丙氨酸的苯環發生疏水作用的區域由ile10 、 ile13 、 pro150 、 leu175 、 leu179 、 phe209和val221的側鏈構成，而asp6 、 asp7 、 gln151和ser180的側鏈構成了與苯丙氨酸的氨基和羧基相互作用的區域。
  14. Structure analysis indicated that the molecule of hwtx - i consists of a small triple stranded anti - parallel ( 3 - sheet and five ( 3 - turns. the n - terminal, c - terminal and most basic amino acid residues are located in the surface of the molecule

    構象研究表明， hwtx -由一小段三股反平行的-折疊和5個-轉角組成，肽鏈的n端和c端以及多數堿性氨基酸殘基都分佈在分子表面。
  15. Human insulin - like growth factor - 1 ( igf - 1 ) is a basic polypeptide containing 70 amino acid residues. its truncated form, i. e. des ( l - 3 ) igf - 1, is three residues shorter than the intact form at n - terminal. igf - 1 has many biological actions such as mitogenic, metabolic effects and others

    =人胰島素樣生長因子1 （ insulin - likegrowthfactori ， igf - 1 ）是一個由70個氨基酸組成的堿性多肽，其截短型比完整型的n端少了三個氨基酸（甘氨酸、脯氨酸、谷氨酸） 。
  16. Computer - assisted analysis revealed there was a signal peptide near the n - terminal of this protein

    軟體預測0rf132有一個信號肽序列（ signalpeptide ） ，最可能的切割位點在和r20之間。
  17. The sequence encodes an open reading frame of 518 amino acids. there is a transit peptide of 74 amino acids in the n terminal of the putative amino acids sequence coded by the cdna

    該序列長為1758hp ，起始密碼子位於84 86hp ，終止密碼子為1658 166fop ，開放閱讀框長為1557hp ，編碼58個氨基酸序列，其中n末端含有74個氨基酸的轉運肽。
  18. The complete human znf325 cdna sequence is 2697bp and contains a 1389 - bp open reading frame ( orf ) that encodes a 462 amino acid protein with 15 zinc finger c2h2 motifs. the complete human znf359 cdna sequence is 3270bp and contains a 1932 - bp open read ing frame ( orf ) that encodes a 643 amino acid protein with an n - terminal krab domain and 16 c - terminus zinc finger c2h2 motifs. the zfp28 cdna sequence is 4104bp and contains a 2076 - bp open reading frame ( orf ) that encodes an 868 amino acid protein with an n - terminal signal peptide, two krab domains and 14 c - terminal c2h2 zinc finger motifs

    Znf325長2697個堿基，含有一個長為1389個堿基的開放閱讀框，編碼一個長為462個氨基酸的蛋白質，它包含15個c2h2型鋅指結構； znf359長3270個堿基，含有一個長為1932個堿基的開放閱讀框，編碼一個長為643個氨基酸的蛋白質，它包含一個n -端krab結構域和16個c -端c2h2型鋅指結構； zfp28長4104個堿基，含有一個長為2076個堿基的開放閱讀框，編碼一個長為868個氨基酸的蛋白質，它包含一個n -端信號肽，兩個krab結構域和14個c -端c2h2型鋅指結構。
  19. The chemical degradation method established by edman in 1950 has been a routine approach for n - terminal sequencing and is widely employed by automated protein sequencer

    運用edman降解進行蛋白質n端順序測定已成為十分完善的技術，並已經實現了自動化。
  20. The former expresses recombinant proteins with a 6xhis tag at n - terminal. the fore mentioned four fragments were all used for expression in this system and the mature protein and the conservative domain were effectively expressed while the expression of the other two was unobvious

    前者表達n ?末端加6 his標記的融合蛋白，克隆到的4個基因片段均進行了表達，其中成熟肽和活性區域得到了大量表達，蛋白全長和末端缺失片段表達不明顯。
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