p-enzyme 中文意思是什麼

The results show that the algaecides can react with the chlorophyl a, the protein and the sod enzyme, and also can destroy the modality of the algae. soluble chitin - iodine was also discussed. the results show that soluble chitin - iodine can remove the p. globosa red tide

通過研究碘伏、新潔而滅和異噻唑啉酮對棕囊藻的生理效應和使用掃描電子顯微鏡觀察棕囊藻的形態結構的破壞情況，探討了這幾種除藻劑的除藻機理。

The stations e2 and 1 - 4 were located at the cold water mass area of the central yellow sea, which characterized by low temperature, high salinity and stable theromocline would generate a retention mechanism that promoted the formation of separate, self - supporting stocks of krill. 2 genetic diversity and differentiation of p. latifrons specimens of p. latifrons were collected from the east china sea and the south china sea. the zymogram phenotypes of aspartate aminotransferase ( e. c. 2. 6. 1. 1, aat ), alkaline phosphatase ( e. c. 3. 1. 3. 1, alp ), a - amylase ( a - amy ), r - amylase ( r - amy ), esterase ( est ), lactate dehydrogenase ( ldh ), raalate dehydrogenase ( mdh ), malic enzyme ( me ), and phosphoglweoisomerase ( pgi ) were scored

（二）寬額假磷蝦遺傳多樣性和遺傳分化研究1 .本文對東海外海和南海2個站位寬額假磷蝦群體進行了分析，在檢測的9個酶系統中，共檢測到11個酶位點:天冬氨酸轉氨酶（ l個位點， 2個等位基因） ，堿性磷酸酶（ 2個位點， a加了和a加2各有2個等位基因） ， r澱粉酶（ l個位點， 2個等位基因） ，醋酶（ 2個位點， es巧和est7各有2個等位基因） ，蘋果酸脫氫酶（ l個位點， 3個等位基因） ，蘋果酸酶（ l個位點， 2個等位基因） ，乳酸脫氫酶（ l個位點， 4個等位基因） ，磷酸葡萄糖轉氨酶（ l個位點， 3個等位基因） ; a澱粉酶為單態。

The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。

Rinat b, hubert c, corvol p, et al. pcr detection of the insertion / deletion polymorphism of the human angiotensin converting enzyme gene j. nucl acids res, 1990, 20 : 1433

和姬苓，王永福，楊國安，等.血管緊張素受體? 1基因多態性與腦血管病的關系j .臨床神經病學雜志， 2007 ， 20 : 12

P - galactosidase of e. coli which is scarce in human blood plasma and has a variety of substrates, is one of the most frequently used enzyme labels. it is used both in heterogeneous and homogeneous enzyme immunoassay

大腸桿菌的-半乳糖苷酶，因人血漿中缺乏此酶，並具有大量易得的底物，被用於均相及非均相免疫分析，是目前最常用的標記酶之一。

Robinson p j, dunnill p, lilly d m. preparation of fine magnetic particles and application for enzyme immobilization. ibid, 1973 ( 15 ) : 603

余藝華，薛博，孫彥，等.殼聚糖親和磁性毫微粒的制備及其對蛋白質的吸附性能研究.高分子學報， 2000 （ 3 ） : 340 344

All the indexes of the second generation in the soil microorganism quantity, biomass, biochemistry activity and the change of soil enzyme activity of p. massoniana under the same condition are higher than those of the first generation

結果表明，二代馬尾松林土壤微生物數量、微生物生物量碳強度、呼吸作用強度、硝化作用強度、蔗糖酶以及過氧化氫酶活性均高於一代馬尾松林。

According to the purity and the activity recovery, the recombinant enzyme was well purified by both of these two separation combinations. like natural gloshedobin, the recombinant enzyme exhibited strong esterase activity using tripeptide p - nitroanilide derivatives as substrate, but hydrolyzed n a - p - tosyl - l - arginine methyl ester ( tame ) or n a - benzoyl - l - arginine ethyl ester ( baee ) very weakly

與天然蛇毒類凝血酶一致，當用三肽p山itroanilide衍生物作為底物時，分泌表達的重組大連蛇島蝗蛇毒類凝血酶具有較強的生色底物活性，但用精氨酸甲酯如tame （ na廠tosyl l ar 。

It was confirmed that the key enzyme of coq10 synthesis is p - hydroxybenzoate polyprenyltransferase, encoded by gene ubia in e. coli and lacks specificity to the aggregation length of its substrate ( polyisoprenyl pyrophosphate ). if gene ubia can be introduced into psb and is high expression in psb, a recombinant strain producing coq10 could be obtained. consequently, the productive cost will decrease sharply

業已證實coq _ （ 10 ）生物合成的關鍵酶為對羥基苯甲酸聚異戊二烯焦磷酸轉移酶（在大腸桿菌中該酶由ubia基因編碼） ，該類酶對底物聚異戊二烯焦磷酸（ ppp ）的聚合長度並無特殊要求。

In the research of transgenic fish, green fluorescent protein gene was sub - cloned to downstream of carp p - actin gene promoter that was cloned in pucusa by molecular recombination technology. thus pagfp plasmid was constructed successfully. the recombination was determined by digestion of restriction enzyme and sequencing

實驗通過分子重組技術，採用定向克隆法將綠色熒光蛋白基因亞克隆到puc118a上鯉魚-肌動蛋白基因啟動子下游，構建成能在真核生物體內表達的表達載體pagfp ，經雙酶酶切法序列鑒定后，回收帶啟動子和目的基因片段。

The hplap induced from the clone with two copies of hplap gene had higher enzyme activity. the clone with more or less copy was unfavourable to the hplap expression in p. pastoris

對部分克隆進行誘導表達發現只有2拷貝轉化子表達產物hplap活性最高，過高過低拷貝數均不利丁hplap的表達。

Mongolica, soil acidity, humus component content, available k, total p, organic p, inorganic p, enzyme activity, and microbe amount of young stand olgensis of the second rotation of larix olgensis in non - rhizosphere soil also

不同發育階段影響林木生長量的主要養分因子是土壤有機質、速效鉀、水解氮以及土壤磷形態的全磷、有機磷、無機磷總量、有效磷、 ca一p和fe一p 。

The transparent rings formed by bacterium decomposing protein between flesh - eating bugs and plant - eating bugs were different. the former were wider than latter. 3. the ratio of amylase to protease enzyme activies ( a / p ) was used as one of the criteria for identifying the feeding habits of bug. the a / p for plant - eating bugs was about 2. 0 - 6. 0, for the flesh - eating bugs was 0. 3 - 0. 8

3試驗採用-澱粉酶活力蛋白酶活力比值作為指標，對蝽類昆蟲進行了食性鑒定，結果表明：肉食性蝽類的-澱粉酶蛋白酶比值范圍在0 . 3 0 . 8之間，而植食性蝽類的-澱粉酶蛋白酶比值范圍在2 . 0 6 . 0之間，食性差異明顯。

3. measuring expression of the associated molecular chaperone grp78 and the enzyme protein disulphide isomerase pdi affected by low expression of grp94. ( 1 ) detecting mrna and p

檢測grp94表達水平下降對相關分子伴侶grp78和pdi的影響（ 1 ）分別通過rt - pcr方法和westernblot方法從mrna和蛋白水平檢測分子伴侶grp78的表達情況。

A novel homogeneous immunoassay system, cloned enzyme donor immunoassay system ( cedia ), has been developed by henderson in 1986 on the basis of a - complementation reaction of 3 - galactosidase ( e. coli ). in the cedia test, genetic engineering was used for the generation and selection of enzyme acceptor ( ea ) and enzyme donor ( ed ) of p - galactosidase

Henderson等人在1986年利用基因工程技術生產大腸桿菌-半乳糖苷酶酶受體（ ea ）和酶供體（ ed ） ，並利用其-互補功能創立了克隆酶供體免疫分析（ cedia ）技術。

The cyp51 genes cloned from both isolates of imazalil - resistant and imazalil - sensitive of p. digitatum were cloned into the pcb 1004 expression vectors, respectively. the direction and number of inserted copy were confirmed by restriction enzyme digestion

本研究構建了指狀青黴對抑霉唑敏感和抗性的兩個cyp51基因的pcb1004表達載體，並對基因的插入方向和拷貝數進行了分析，證明了兩個載體連接的基因均為正向連接和單拷貝插入。

In this thesis, the hplap gene was cloned into the ppiczaa plasmid and induced expressed in the protease - deficient strain p. pastoris smd1 168. the clone that could secrete hplap with enzyme activity was selected

本研究將hplap基岡兌隆到spiczoa質粒中並在畢赤酵母屍pastorjs蛋白酶缺陷菌株smdi168中誘導表達，獲得了有活性的分泌型hplap 。