pcr lab 中文意思是什麼

pcr lab 解釋
實驗室
  • pcr : PCR ＝polymerase chain reaction 【生物化學】聚合酶聯鎖反應〈此項技術可用以復制犯罪現場發現的DNA小樣，從而有助於破案〉。
  1. Part 1 : identification of a novel gene, tsarg2, and its sequence character cloning new apoptosis - related novel gene is a key to further understanding of apoptosis mechanism and the biological process of germ cell, and it is of momentous significance on clarifying physiology and pathology process of spermatogenesis. to rapidly attain human novel gene full - length cdna sequence, the gene - specific primers and the vector - specific primers have been designed for successful performing nested pcr and draft human genome searching to rapidly identify the tsarg2 ( genebank accession number ay040204 ) 5 " end from a human testis cdna library by using a cdna fragment ( genebank accession number be644542 ) as a electronic probe, which was significantly changed in cryptorchidism and represents a novel gene. furthermore, a mouse homologue of this gene was identified ( genebank accession number af395083 ) by lab on - line

    本研究分為三個部分，其主要實驗方法及實驗結果如下：第一章tsarg2基因的克隆與序列分析從已獲得的在隱睪和正常睪丸對照中表達量有明顯差異的est片段（ be644542 ）入手，設計了基因特異性引物和載體特異性引物進行巢式pcr擴增，結合人類基因組草圖搜索法，從睪丸cdna文庫中快速分離出人類睪丸凋亡相關基因的5末端而獲得全長cdna ， genbank登錄號為ay040204 ，同時應用生物信息學的方法克隆了該基因在小鼠中的同源基因， genbank登錄號為af395083 。
  2. The dna encoding the desired protein was generated as a bamh i - sal i fragment using pcr. the template was pbv220 - hgf - a strand that had been constructed in our lab

    其中gst是一種純化標簽，可以在後續的蛋白分離純化中使用gst親和層析的辦法純化目的蛋白，這將使純化工作變得簡單而有效。
  3. In an optimal situation, for three rooms ( reagent preparation, sample preparation, amplification ) of the pcr lab one buffer area could be set up

    在理想情況下， pcr實驗室試劑準備、標本制備、擴增三個區域可設置緩沖間。
  4. In the past, we cloned the cdna of zmcdc5 based on the studies on difference of gene expression in shoot and radicle of maize seedling in our lab and then got the whole genome sequence by maize genomic library screening and inverse pcr

    本實驗室根據玉米胚芽和胚根差異表達的研究，克隆得到zmcdc5基因的cdna ，並通過篩庫和反向pcr獲得了zmcdc5基因組全長序列。
  5. We cloned osftszi reported in rice by rt - pcr and the crftszi in chlamydomonas by bioinformatic technique and lab works

    首先利用rt - pcr技術克隆了已發表的唯一一個水稻osftsz1基因。
分享友人
分享到 Line

分享到臉書