pcr primer 中文意思是什麼

pcr primer 解釋
pcr引物
  • pcr : PCR ＝polymerase chain reaction 【生物化學】聚合酶聯鎖反應〈此項技術可用以復制犯罪現場發現的DNA小樣，從而有助於破案〉。
  • primer : n 1 初級讀本，初學書；初階，入門（書）；（尤指宗教改革前的）小禱告書。2 【印刷】10點或18點活字的...
  1. By means of discontinuous tris - hcl buffer system and poly aery 1 amide gel electrophoresis ( page ), zymogram of 23 species of 11 genera in 4 subfamilies mirinae, orthotylinae, phylinae and deraeocorinae are gained. by specific primer pcr and sequencing, 57 cyt b gene 433bp sequences are obtained from 34 species respectively belonging to 17 genera in 5 subfamilies mirinae, orthotylinae, phylinae, deraeocorinae, bryocorinae from 64 species of 23 genera which involved in this research, and 2 outgroup species of family anthocoridae as well. 1

    首次通過特異引物擴增和基因測序的研究方法從被試的5亞科23個屬的64種盲蝽中獲得了盲蝽亞科、合墊盲蝽亞科、葉盲蝽亞科、赤爪盲蝽亞科和蕨盲蝽亞科bryocorinae5個亞科17個屬的34種盲蝽以及外群花蝽科anthocoridae2種花蝽的cytb基因序列57條。
  2. Considering of the specificity of the degenerative primer designed in this pcr reaction, the identity between the sequence we wanted and the fragment of pcr product and the presence of asmaspa, asmaspb, clr and cls ( the homologous gene of masp gene ) in halocynthia roretzi, a japanese ascidian, we believe that the sequence of pcr product is some part of the masp gene or masp homologous gene

    基於本實驗中所設計的引物為特異性簡並引物，測序基因通過比較得到與預期片段有一定的同源性以及masp同源物asmaspa 、 asmaspb 、 clr和cls在海鞘中存在的事實，我們可以初步推測，本實驗pcr反應所克隆的片段可能為文昌魚masp或其同源基因的一部分序列。
  3. The tailed primer design protocol is feasible for y - str loci and can be used to develop more multiplex pcr assays of y - str loci. the results of validation prove that the y - a489 - plex multiplex pcr system can be accepted for the forensic dna typing

    證明y一a489一plex ，適合於法醫dna分析，有較高的應用價值，為法醫y染色體分型提供了新的技術手段。
  4. To study on structure and inheritance of rh d gene interaction between gene expression of rh d and rh c / e and influences on rh d gene expression of inserts and rh box - methods : 20 pairs of oligonucleotide specific primers for exon, intron 2 4, insert and rh box of rh d, rhc / e gene were designed and composed the polymerase chain reaction - sequence specific oligonucleotide primer ( pcr - ssp ) was used to amplify the rh c / e gene, rh d gene, exon, intron, insert and rh box in 106 samples of unrelated individuals and 7 han nationality ancestries and 5 wei nationality ancestries whose proband were rh d - negative

    目的：觀察中國漢族非血緣關系隨機個體、漢族及維吾爾族家系rh血型的c e基因、 d基因外顯子、內含子、插入片段以及rhbox ，研究rhd基因結構及遺傳規律， d基因表達與c e基因的關聯，以及插入片段和rhbox對d基因表達的影響。
  5. 10 phenons at the similarity level of 80 %. the fingerprints of rep - pcr which conducted by boxair primer showed that the isolates from the root nodules of pueraria spp. had g reat genetic diversity. they were divided into 11 genetic clusters at the similarity level of 81 %

    以boxair為引物的rep - pcr分析結果表明葛藤根瘤菌存在不同水平的遺傳多樣性，在81的相似性水平上，供試葛藤根瘤菌分成了11個遺傳群，其結果證明了慢生葛藤根瘤菌的遺傳多樣性。
  6. In this paper, total dna from tannage, tail skin, scales and the skin treated with salt were successfully extracted with an improved method for dna extraction. to verify the results, four pairs of primers, which were universal primers for 12s rrna gene, diagnostic primers of chinese alligator meat, microsatellite primers and rapd primer, were used to do pcr amplifications. some amplified fragments were sequenced, either

    本文研究出一種改進的dna提取方法，成功地從揚子鱷鞣製皮革中提取了總dna ，同時對不同的組織標本如鱗片、鹽腌生皮及尾尖皮等進行了dna提取：並利用12srrna基因擴增的通用引物、揚子鱷鑒定性引物、微衛星引物及rapd引物進行pcr擴增，且對部分pcr擴增結果進行測序，以檢驗提取效果。
  7. We confirmed that the cloned gene dreb1c was really transformed into arabidopsis by pcr method, in which sequences of camv 35s were used as forward primer

    提取轉基因植株的基因組dna ，進行pcr反應，證明了dreb1c確實轉入擬南芥中。
  8. Based upon the comparison of cyto b gene sequences in 15 deer species downloaded from genbank, a universal primer set l15774 / hsf21 was used as positive control of the template quality, at the meantime, two species specific primer sets df / dr and cf / cr were desgined to identify red deer ( cervus elaphus ), sika deer ( cervus nippori ) and roe deer ( capreolus capreolus ) from other species. a musk deer ( moschus ) specific primer set wf / mr was designed, siberian musk deer ( afoschus moschiferns ) and forest musk desr ( moschus berezovskii ) could be discriminated when restriction endonuclease rsa i was used to cut the pcr products

    經過對來自genbank中的15種鹿類動物的cytob基因序列的比較，用通用引物l15774和hsf21作為模板的質量控制，設計了特異性引物df dr和cf cr來鑒定馬鹿、梅花鹿、狍；設計了麝類特異性引物mf mr ，用限制性內切酶rsa酶切擴增產物來區分原麝和林麝。
  9. In order to identifiy the virus further, a set of double nested primers for canine coronavirus was selected. the primers were designed in s gene region from ccv including two pairs of primers : ccvfl - ccvrl, ccvf2 - ccvr2. the first is a pair of outer primer, and can amplify a fragement of 1086bp. the second is a pair of inner primer. and can amplify a fragement of 515bp. using the nested primers, many ccv strains can be identificated including k378, insave - l, ccv 1 - 71 etc. synthesizing this set of primers, we selected the panda ' s liver - tissue materials and some different passages of viral culture to amplify by rt - pcr, and all of them respectively gained two target fragements of 1086bp and 515bp, but the control cell did not

    合成該套式引物，選擇大熊貓原代病料和病毒各代細胞培養物，經套式（ nested ） rt一pcr擴增，可得到一與設計值5巧bp相符的dna片段，經bst一xl （ 590 ， 1110 ）酶切鑒定，證明該擴增片斷為特異性片段;回收大熊貓肝組織原代病料和細胞培養物第2 、 3 、 29代的ccvfz一ccvrz擴增片段，純化，送生物公司測序。
  10. Two plasmids, which contain hbv specific dna fragment and hsv1 dna fragment, were amplified by solid phase two loci pcr and detected by enzymatic indicator system on a gene chip that was constructed by primer immobilization and modified with thiol group on chip surface. for building detection technology by dna chip in clinical, the virus genomes were extracted from the clinical positive samples by one - step nucleic acid extraction

    採用已建立的晶元制備方法，在該六種質粒中選擇含有hbv與hsv1兩種病毒dna序列的質粒為模板，進行同相兩重pcr ，採用晶元酶學槍測對固相兩重pcr的擴增結果進行檢測，得到良好的晶元酶學檢測結果。
  11. Concatemer were constructed by polymerization with t4 dna polymerase and primer - templated pcr with high fidelity dna polymerase. one concatemer of 360bp, 9 copies of grb - ast3, were obtained from the first pair of primer and three ones of 515bp, 750bp, 792bp, from the second one

    從第一對引物的拼接產物中克隆到一個360bp含有9個拷貝數的grb - ast _ 3的串聯體，從第二對引物的拼接產物中克隆到三個分別為515 、 750 、 792bp的grb - ast _ 3的串聯體。
  12. The divergent sequences of rdna from s. costatum are used to design primers meeting the requirements of the rfq - pcr. seven pairs of primers are designed and designated as primer 1 ( f / r ) ~ primer 7 ( f / r ), respectively, among which primer 1 ( f / r ), primer 2 ( f / r ), primer 5 ( f / r ), primer 6 ( f / r ) showed high level of specificities to s. costatum. then, the pcr products primed by primer6 ( f / r ) are sequenced

    首先以中肋骨條藻的rdna序列為設計種特異性引物的靶區域，共設計出7對適合rfq - pcr的引物（依次命名為primer1 （ f r ） primer7 （ f r ） ） ，並用常規pcr實驗初步證明其中有4對引物（ primer1 （ f r ） 、 primer2 （ f r ） 、 primer5 （ f r ） 、 primer6 （ f r ） ）可作為中肋骨條藻特異性引物的候選者；然後測定了以primer6 （ f r ）為引物的pcr產物的序列，序列分析表明，中肋骨條藻的pcr產物序列與其他藻的pcr產物序列差別較大，從中可設計出滿足rfq - pcr需要的taqman探針（命名為taqman6 ） ；進一步的核酸雜交實驗表明， taqman6隻與中肋骨條藻的pcr產物雜交，不與其他藻的pcr產物雜交。
  13. We found that sit variant gene ( slt2vha ) was identified in strains e. coli o157 : h7 isolated from patients and dung beetles 2000 in xuzhou city, jiangsu province. the primers used for stx2 variant analysis are shown in tablel. genomic dna restriction fragments digested by pstiwere sonthern - blotted and hybridized with an stx2 - specific dna probe. the probe was prepared fromed a 285 - bp pcr productof the strain 882364 stx2 gene obtained by using the specific primer pair ( tablel )

    2000年在江蘇省徐州市銅山縣腹瀉病患者的糞便標本分離的10株產生志賀毒素的菌株以及從蜣螂腸道分離到的4株產生志賀毒素的大腸桿菌o157 ： h7 ，屬于另兩個pfge型，和1986 、 1987 、 1988年在徐州市腹瀉病患者的糞便標本分離的菌株pfge圖譜不同。
  14. Clone human wnk4 full length cdna into pgem - t vector human kidney total una was extracted and used as template to amplify wnk4 full length cdna with the forward primer ( 7 - 27 ) and reverse primer ( 3808 - 3833 ) with the long template expanded pcr system kit

    5 .原位雜交以小鼠腎臟總翩a為模板，擴增wnki基因外顯子1 ， 24一28 ，以a片段和wnk4基因外顯子13一16片段，純化后連接在pgem一t載體上。
  15. Primer - introduced restriction analysis - pcr, pira - pcr

    以引物引入限制性內切酶分析聚合酶鏈反應
  16. Constructing cdna expressing library of erythrocytic plasmodium falciparum from hainan : the total rna was obtained by using. triplix kit. a modified oligo ( dt ) primer ( cds ffi pcr primer ) was used in the single - stranded ( ss ) dna synthesis reaction. the ss - dna was reversely transcripted from total rna. double - stranded ( ds ) cdna was amplified by long - distance ( ld ) pcrafter the digestion with proteinase k and sfi i, the cdna with no less than 200bp was collected and purified by glass - milk kitthe library was constructed after the ligation of cdna to tiplex2 phage particle packaged with the packaging extract system in vitro. a high titer and high recombinant ratio of cdna library was constructed

    構建惡性瘧原蟲海南株紅內期cdna表達文庫提取紅內期惡性瘧原蟲海南株總rna ，直接以總rna為模板使用cdna文庫構建試劑盒，首先反轉錄合成ss一dna ，再擴增合成ds一dna （ cdna ） ，對擴增產物用蛋白酶k消化及左z丁i酶切，抽提蛋白、去除rna后，用玻璃奶試劑盒純化、回收20obp以上的片斷，經與載體連接再用蛋白包裝物包裝后形成未擴增文庫，最後擴增完成惡性瘧原蟲海南株紅內期cdna表達文庫的構建。
  17. Results show that pcr primer selection also plays decisive role in the methodology. two primer pairs considered universal to domain bacteria in previous reports did not work on our field sample

    Dna提取和pcr的空白試驗表明，本方法容易受到污染的影響，對實驗材料和無菌操作的要求必須嚴格。
  18. To device a primer pairs and amplify the full length pstvd by rt - pcr, the positive rna extraction from tuber sample was used as template. the product of rt - pcr was purified and connected to the plasmid pmd 18 - t vector

    Cdna核酸斑點雜交反應（ nash ）檢測pstvd方法準確、靈敏度高，一次檢測樣品數量多，且對于異地樣品檢測非常方便，是以往其它檢測方法的有效補充。
  19. In order to check if it is the aim gene, we devised pcr with a new pair of primer, sequenced and registered the product with registration number : af449446. moreover we forecast and analysis the primary, secondary, tertiary and quaternary structure of the three protein : osftszi, crftszi and crftsz2 which has already cloned by our team before. after that we construct ftsz molecular evolution tree to site them in

    又將生物信息學技術同實驗技術相結合，針對ftsz保守區設計引物擴增出一條衣藻ftsz片段，進行est搜索、比對、拼接，最終克隆出新基因crftsz1 ；連同本試驗室曾經獲得的另一個衣藻crftsz2基因進行蛋白質的一、二、三、四級結構預測、分析及比較尋找進化線索，建立了ftsz蛋白的氨基酸進化樹作進一步的進化定位。
  20. To make the pcr primer that have adequacy enzyme sit flowed the sequence, ligate with the pgem - t easy after pcr. make sure the sequence by sequencing

    經組織培養后，以pcr和rt - pcr法檢測再生煙草，共得到轉synnhap基因煙草4株， pbi121空載體浸染的對照煙草再生苗5株。
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