plaque hybridization 中文意思是什麼
plaque hybridization
解釋
噬菌斑雜交- plaque : n. 1. （象牙、陶磁等制的）飾板；匾。2. （表示地位、名譽的）胸章；徽章。3. 【醫學】斑；（噬菌體）溶菌斑；血小板。
- hybridization : 混成作用
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The pbacph - egf plasmid dna was used to co - transfect bmn cell with the modified bombyx mori baculovirus bm - bacpak dna, which was first linearized by aocl. after two rounds of plaque isolation, the recombinant virus ( named bmbacph - egf ) was further verified with pcr and dot hybridization. the recombinant bmbacph - egf virus was used to infect bmn culture cells at a moi of 10
重組轉移載體dna與經bsu36i酶切線性化的修飾型病毒bm - bacpakdna共轉染家蠶bmn細胞，兩輪空斑篩選和純化，獲得重組病毒rbmbacph - egf ，經pcr擴增、 dna點雜交等方法鑒定證實重組病毒中已正確插入ph - egf融合基因。 -
Firstly, a reverse hemolytic plaque assay ( rhpa ) was used to scale testosterone secretion of leydig ' s cells in single cells level. sequentially, star mrna expression was detected with fluorescent in situ hybridization ( fish ) at the same cells
本研究首先採用逆向溶血斑法，鑒定單個間質細胞睪酮分泌上的差異，然後，在被鑒定的同一間質細胞上進行原位雜交（ fish ） ，以便在單個細胞水平上確定間質細胞是否存在功能及star基因表達的異質性。
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