plasmid engineering 中文意思是什麼

plasmid engineering 解釋
質粒工程
  • plasmid : 質粒；原核質體
  • engineering : n. 1. 工程（技術），工程學。2. 開車技術。3. 土木工程，工事。4. 操縱，管理。
  1. 4. engineering dhqase ( arod ) - deficient e. coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e. coli chromosome. the mutant 31bk was engineered, in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e. coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system. the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene, so it improved carbon flow into the quinic acid biosynthesis direction

    構建宿主菌基因精確定位突變株31bk （ arod : : arobkan ~ r ）為了改變代謝途徑脫氫奎尼酸（ dhq ）分支點上的代謝流量，使之充分流向目的產物奎尼酸合成方向，利用基因打靶技術構建了31884宿主菌arod基因精確定位插入突變體，使dhq脫水酶（ dhqase ）失活，阻斷了碳代謝流流向芳香氨基酸生成的方向，同時用同源重組的方法將arob基因定位整合入染色體上，解除了限速酶對碳代謝流通過共同途徑到達dhq的阻遏影響，並減輕代謝負擔。
  2. Since the plasmid is capable of independent replication in host cells of many dicotyledonous plants, it has been used as a cloning vector in gnetic engineering

    在許多雙子葉植物中質粒在寄主細胞中是獨立的復制單元，所以可以在基因工程中用作克隆載體。
  3. By genetic engineering methods, ureb gene and ureb - hspa fusion gene were amplified by pcr and cloned into a prokaryotic expression plasmid ptrc99a - asd, and identified recombinant plasmid was then

    口服、鼻飼免疫balb / c小鼠，在腸液和血清中可以分別檢測到針對hpylori的特異性分泌型iga和址g抗體。
  4. Acetylornithine deacetylase is the key enzyme of producting l - methionine. we mainly do research work on the construction of acetylornithine deacetylase gene - engineering strain and characteristic of proteinase. in order to get high expression deacetylase strain, we obtain the gene by pcr arge gene. the product ( 2800bp ) was cloned into puc19 plasmid and confirmed with blue / white dot screening > restriction enzyme analysis and pcr. then taking the nucleotide sequencing compared with the sequence at blast of u. s. a. we constructed a high expression of gene - engineering strain - pxj 128 which containing the arge gene on the high expressing system of pxji18 with activity of acetylornithine deacetylase above 20000u / g

    為了獲得高效表達的脫乙酰鳥氨酸酶工程菌株，在工程菌技術改造及其固定化研究做了進一步的研究和探討。我們採用基因工程技術，通過pcr技術擴增出了酰化酶關鍵酶基因?脫乙酰鳥氨酸酶基因arge ，將其克隆到puc19載體中，經酶切鑒定、 pcr鑒定篩選出重組陽性質粒，並測序鑒定，通過美國blast程序進行了基因數據庫相似性比較分析。
  5. In this study, we have constructed a new cea dna vaccine and a plasmid of il - 2 by using genetic - engineering technology, and designed the immunostimulatory dna sequence ( iss ). we hope enhance the quantity of cea and the anti ~ cea by using the various vector, cytokine and iss

    本文運用基因工程技術構建了cea的dna疫苗pc工cn ，白細胞介素七的真核表達質粒pci可l 《 ，設計合成了硫代修飾的免疫刺激序列門ss ） ，希望從載體、細胞因子和es方面提高cea的表達和抗ea的產生，從而達到對優a陽性腫瘤免疫防治的目的。
  6. ( 3 ) establishing a new method which can quantitatively determinate telomerase activity. 1. human telomerase rna component ( htr ) gene was cloned from hela cells using gene engineering technology. the htr gene was then reverse inverted into retrovirus vector plncx and constructed an antisense recombinant plasmid plncx - atr

    將htr基因插入逆轉錄病毒載體plncx中，構建了htr基因的反義重組質粒plncx - atr和正義重組質粒plncx - tr ，測序結果表明符合預期要求，為下一步實驗奠定了基礎。
  7. Purification of the recombinant expressed endostatin in this work we have successfully cloned mouse endostatin gene, and constructed pbv220 - endostatin expression plasmid that is a non - fusion expression vector. the positive pbv220 - endostatin was transformed into dh5a, and expressed mouse endostatin protein successfully. this study is the basis of the endostatin gene - engineering production

    本文成功克隆鼠內皮抑素基因，構建了內皮抑素基因的原核表達載體pbv220一endostatin ，該載體為非融合性表達載體，將pbv22o一endostatin成功轉化到大腸桿菌表達菌dh5a中，並誘導表達了鼠內皮抑素蛋白，為基因工程方法生產內皮抑素奠定一定基礎。
  8. More interesting, the content of secondary metabolite always increased markedly in hairy root. so the transgenic system by ri plasmid would be wildly used in plant breeding, plant gene engineering and pharmaceutical element production

    發根農桿菌遺傳轉化體系的這一特點在作物育種、植物基因工程、次生代謝物工廠化生產上有廣闊的應用前景。
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