polyhedrin 中文意思是什麼
polyhedrin
解釋
多角體蛋白-
In the experiments discussed in chapter 5 we generated two recombinant viruses based on an acmnpv - and hasnpv - bac - to - bac system, respectively. in such recombinant viruses the busuctl gene under polyhedrin promoter was inserted into polyhedrin gene locus. a preliminary bioassay was conducted
第五章利用桿狀病毒bac - to - bac系統構建了含有油桐尺蠖核多角體病毒的類蝸牛毒素基因的重組病毒racbacctl和rhabacctl ，在其相應宿主甜菜夜蛾和棉鈴蟲的細胞水平和蟲體水平進行了超表達實驗。 -
Two types of repeat sequence, a 15 - amino - acid ( eelcaqlcstppppi ) with 2 repeats and a 6 - amino - acid ( ppictp ) with 4 repeats, were firstly reported. 2. the characterization of meq gene product and its expression within the cells a recombinant baculovirus transfer vector pblubac4 - meq was constructed by cloning meq gene of marek ' s disease virus ( mdv ) ga strain into the baculovirus transfer vector pbluebac4 under the polyhedrin promoter
此外，研究還發現了meq基因的兩類有趣的重復結構，其中一類是含15個氨基酸殘基（ eelcaqlcstppppi ）的結構，有2個重復，另一類是含6個氨基酸殘基（ ppictp ）的結構，共有4個重復，它們全部分佈在meq蛋白c -端的轉錄激活域內。 -
To obtain large amounts of appa phytase, the appa gene was subcloned into the prokaryotic expression vector pet - 28a ( + ) and baculovirus transfer vector pvl - 1393 under the control of the lac and polyhedrin promoter, respectively. the appa phytase was overexpressed in e. coli strain bl21 induced by lactose
為了大量表達appa植酸酶，我們將appa基因分別克隆至原核表達載體pet - 28a （ + ）和桿狀病毒轉移載體pvl - 1393中，將其分別置於lac和polyhedrin啟動子控制之下。 -
The polyhedrin gene sequence in recombinant virus bmpak - hbmp can be more benefit to enhancing the expression of the hbsag ( pres2 + s ) from atg initial codon at the 5 " ends of pres2 gene
另一方面， elisa檢測表明， presz抗原性提高率約是hbsag的14倍，提示bmpak hb帥中多角體基因部分堿基序列的存在更有利於從presz前的atg開始表達中蛋白全基因（ presz s ） 。 -
A 12 bp sequence of the 5 " end from the polyhedrin protein gene of bmnpv was ligated to the 5 ' end of hbsag ( pres2 + s ) protein coding sequence by pcr. the fusion product coding for hbv surface antigen medium sized ( hbmp ) with 4 - additional aa of bmnpv polyhedrin protein was obtained
本研究通過pcr突變的方法，在hbsag （ pres2 + s ）前s2序列的5 』端融合了bmnpv多角體蛋白基因5 』端的12個堿基，獲得了融合乙肝表面抗原中蛋白基因（ hbmp ） 。 -
Hegf gene with his - tag at the end, which was derived from pet22 - egf, was in - frame fused to the carboxy - terminal of polyhedrin ( ph ) gene, which included the amino - terminal 116aa coding region. the polyhedrin - egf fusion gene ( named ph - egf ) was then cut out with ecorv and ecori, and was cloned between ecorv and ecori sites of pbacpak. 8 ( the result plasmid was named pbacph - egf and the ph - egf fusing gene was right under the control of ph promoter ). the pbacph - egf structure was verified with restriction enzymes digestion and pcr
從pet22 - egf質粒中分離出末端帶his - tag的egf基因，對位融合於多角體蛋白n端116個氨基酸基因序列的下游（命名為ph - egf ） ，並在兩段基因間設計了凝血酶xa蛋白酶切位點，經過酶切、測序等鑒定正確后，克隆至pbacpak8中，使ph - egf融合基因置於多角體蛋白（ polyhedrin ， ph ）基因啟動子控制之下，構建成重組轉移載體pbacph - egf 。 -
To solve this problem, we used bac - to - bac system to recombine gfp - actin fusion gene and gfp gene under the control of the promoter of polyhedrin gene respectively. two recombinant virus : acmnpv - gfp - actin which contains gfp - actin fusion gene and acmnpv - gfp which contains gfp gene were obtained. the latter was set as a control
為了解決以上問題，在以上實驗的基礎上，我們利用bac - to - bac系統分別將gfp基因和gfp - actin融合基因重組于多角體基因啟動子之下，構建了兩種重組病毒，即含gfp - actin融合基因的重組病毒acmnpv - gfp - actin和含gfp基因的重組病毒acmnpv - gfp 。
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