protein antibody method 中文意思是什麼

protein antibody method 解釋
蛋白抗體方法
  • protein : n. 【化學】朊，蛋白（質）。
  • antibody : n. 【醫學】抗體。
  • method : n 1 方法，方式；順序。2 （思想、言談上的）條理，規律，秩序。3 【生物學】分類法。4 〈M 〉【戲劇】...
  1. A novel protein immobilization approach for piezoelectric immunosensors based on the sam of derivative aa has been proposed, which is formed by coupling merca ptoprorionic acid ( mpa ) to alginic acid sodium salt via edc and nhs. ( 1 ) the tr antibody is immobilized via edc and nhs. comparing with the mpa sam method, this new approach shows greater frequency response and high sensitivity

    應用edc和nhs使褐藻酸鈉與胱胺偶合形成衍生褐藻酸鈉作新的自組裝材料，用於蛋白固定，應用這一新方法， （ 1 ）實現了轉鐵蛋白抗體分子的固定，並對轉鐵蛋白參考血清進行了測定，該方法可在0 . 08 25 . 7 g / ml范圍內對轉鐵蛋白進行測定。
  2. The determination of human thymidine kinase ( htk ) in human serum, which is a key indicator of cancers can give information for the diagnosis and treatment of the malign diseases. the protein a layer was first self - assembled onto the gold electrode surfaces of quartz crystals, the monoclonal antibodies were then orientedly immobilized through the specific binding between the fc terminals of the antibodies and the self - assembled protein a. with this sensor, the affinity constant of antigen - antibody binding was estimated to be 1. 85 106 l / mol according to the scatchard ’ s plotting method, which proved the high bioactivity of antibody. finally, an amplified piezoelectric immunosensor was designed to determine the htk in

    實驗中將蛋白a吸附於鍍金壓電石英晶體電極表面，用於定向固定htk單克隆抗體，成功研製了檢測htk的壓電石英晶體傳感器，並基於標準scatchard繪圖法，計算出免疫反應的親和常數為1 . 85 106l / mol ，證明該單克隆抗體具有較高的免疫活性;同時基於酶催化沉澱技術，設計了的檢測htk的質量放大壓電石英晶體傳感器，該傳感器可在0 . 1 - 10ng范圍內對htk進行定量檢測，應用此傳感器成功地對5種癌癥病人血清中htk的濃度進行了測定，實驗結果為癌癥的臨床診斷與治療提供了參考。
  3. After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s

    通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。
  4. The extracellular 100pl ( ecl1 ) and ecl2 was linked by disulfide bond to maintenanced the stability of the protein secondary structure. in recent years, we showed that ccr5 as a co - receptor could interact with hiv - 1 infect ion. ccr5 was paid closed attention to since it was cloned in 1996. the aim is to - obtain the sequence of first extracillular domain of 3 chemokine receptor 5 ( ccr5 ) n - terminal gene fragment with high level expression in e. coli and to prepare its specific antibody f ( ab ' ) ;, and its detected method

    Ccr5具有g蛋白偶聯受體家族（ g - proteincoupledreceptors ， gpcrs ）所特有的7個跨膜區（ transmembrinedomains ， tm ） ，呈螺旋， tm的氨基酸有很高的保守性，膜外第一襻（ extracellularloop1 ， ecl1 ）和ecl2之間有二硫鍵相連，以維持蛋白質二級結構的穩定性。
  5. Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry

    三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質
  6. The recombination vectors were transformed into host strain of bl21 and induced with iptg. all the recombinant protein was expressed into inclusion body. the recombinant protein was identified with sds - page and westen - blot and purified through elution method. the muticlone antibody was got by immuning the rabbit with the purified protein

    把重組質粒轉化入表達受體菌bl21 ，經iptg誘導后都獲得了表達，且都以包涵體的表達形式存在，用sds - page和western - blot的方法對重組蛋白進行了鑒定。
  7. With the base of related research on nucleic acid immunization, the technology was used to develop th e research and application of cpti transgenic plant. a new antibody preparation method by nucleic acid immunization to assay the expression of the transgenic gene was explored and compared with the traditional one which immune animal by protein

    在國內外大量核酸免疫的研究基礎上，本研究首次將核酸免疫技術應用於轉基因植物檢測研究中，探討一種核酸免疫法制備抗cpti抗體來檢測基因表達的方法，並與傳統的蛋白免疫方式制備抗體進行比較。
  8. This new method plays an important role in the field of basic biological research, medical diagnosis and clinical therapy. because of its high - sensitive and specific interaction between macromolecules, spr biosensor is used for directly examining the reaction between targeted protein and corresponding antibody without careful purification

    Spr生物傳感器的高靈敏性，以及生物大j皮巴」 a碩士芹仁論文v沉孟y吁」匹r 』 sn正「分子間反應的高度特異性，使不需要嚴格純化而直接檢測目的蛋白與抗體間的反應成為可能。
  9. A dot - ppa - elisa method has been developed to detect the antibody in swine serum against pasteurellosis. the capsule polysaccharide and outer membrane protein of pasteurella multocida was prepared as the diaphragm antigen

    本研究提取豬巴氏桿菌菌株的莢膜和外膜蛋白抗原，首次建立了檢測豬巴氏桿菌病血清抗體的dot - ppa - elisa方法。
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