puc 中文意思是什麼
puc
解釋
普茨-
4. the construction of middle - clone vector and expression vector the puc - cp and pgem - 7z plasmid were digested by kpnl and bamhi, and collected the digested cpti fragment and the pgem - 7z, then ligated by t4 dna ligase and formed the pgem - cp
中間載體及表達載體的構建將puc - cp質粒和pgem ? 7z質粒,用kpni和bamhi酶切,分別回收cpti片斷和酶切后的載體片段,用t _ 4連接酶連接構建成中間載體pgem - cp 。 -
The amplified cdna fragment was inserted to plasmid puc 18. the sequencing result shows that length of cgh cdn a fragment is 669bp
正反向測序結果分析表明:克隆的草魚生長激素基因cdna全長669bp ,開放閱讀框( orf )包含633個堿基,編碼210個氨基酸。 -
Selected one of the 14 strains - s93, s93 dna was digested partially with sau3a i and 2 ~ 3kb fragments were collected and inserted into puc 18, then transformed into dh5 a. filtering the clone with hybridization in situ, a 1 kb frament clone has been cloned
使用sau3ai對基因組dna進行不完全酶切,回收2 3kb片段,與puc18質粒連接轉化大腸桿菌,利用地高辛標記探針,使用菌落原位雜交篩選轉化子;篩選到包含有約1kb外源片段的轉化子。 -
After absorption, the all - frans - retinal isomerizes to a 13 - c / s configuration and br undergoes a photocycle : br570 k590 l550 m410 n520 o640 br570 bacteriohodopsin is a promising biological photoelectric material. we intent to conduct a more thermostable br by site - directed mutagensis. a point ( no. 274 t, no. 274 a turn to c g ) mutation was introduced to br gene by successive pcr technique, and cloned into puc - 19 vector
本論文利用連續pcr的方法定點突變了br基因的一個氨基酸,突變位點選擇了br基因第273的t和274位的a ,把它們變為cg ( tyr替換為arg ) ,然後把突變的br基因克隆入嗜鹽菌表達載體pnov - r ,經測序鑒定的表達載體轉化br缺陷的嗜鹽菌,構建了tyr79 argbr突變體。 -
Within the limits of puc the combined demographic social and economic forces give rise to hyper speed urbanization at an unseen scale
在中國人民的城市之中,人口統計結合社會與經濟的力量使得的城市化進程在一個不被人所見的規模下迅猛發展。 -
The concept of puc introduces a paradox
中國城市化的概念是矛盾的。 -
Ab initio study on potential energy function and vertical ionization potential for puc molecule
分子的結構與垂直電離勢的理論計算 -
Puc pickup car
客貨兩用小汽車 -
All of our vehicles are fully insured with a us 10, 000, 000 insurance policy and we operate under the authorities of puc icc
我們所有豪華旅遊巴士都擁有美國公共事業委員會puc和聯州商務委員會icc的認可並購有一千萬美圓的意外保險。 -
To arouse public awareness of the value of sport and physical recreation, the provisional urban council ( puc ) organised 9249 grassroots training and recreation programmes for 648465 participants of all ages and abilities at a cost of $ 39. 23 million in 1997
臨時市政局為使市民體會體育和康樂活動的價值,在一九九七年耗資3 , 923萬元,舉辦了9249項基層訓練和康樂計劃,供648465名不同年齡和體能的人士參加。
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