相同段片 的英文怎麼說

中文拼音 [xiāngtóngduànpiān]
相同段片 英文
identical sections
  • 相 : 相Ⅰ名詞1 （相貌; 外貌） looks; appearance 2 （坐、立等的姿態） bearing; posture 3 [物理學] （相位...
  • 段 : Ⅰ量詞（部分） section; segment; part; paragraph; passage Ⅱ名詞（姓氏） a surname
  • 片 : 片構詞成分。
  • 相同 : identical; the same; alike; equal
  • 段片 : fragment
  1. The sequence analysis revealed that the as1 gene encodes a myb protein, which is a candidate transcription factor. in as1 and as2 mutants, the polarity formation in leaves is defective. cell differentiation along abaxial - adaxial, proximal - distal and media - lateral axes all shows an insufficient fashion

    通過掃描電鏡、干涉相差顯微鏡、組織切片、過量表達等手段研究了as1和as2的功能，包括觀察觀察突變體的組織、細胞結構及早期發育狀況，同時採用gus表達、 rt - pcr 、原位雜交、 northern等手段分析基因的表達情況。
  2. Construction and expression of yeast engineering yaccine : s14 / gnsag " as transformed to yeast host straln x33 by means of electroporation after ppiczaa s, . aresag " as l ined by saci enzyme. the single fungus, as choose and dibble inocu1ating in we and am plate, the positive fungus was gro ' ing in rm but not in w, and was 6 inoculated in ypd which included zeocine 500ug / ml and 1000ug / ml. 5 transformers were ampl if ied by pcr, three is same with positive control

    選取單個菌落分別點種到刪平板和md平板，找出在回d上生長正常， w上生長緩慢或不生長的菌落，即陽性菌落，再以陽性菌落分別塗布zeocine含量500ug加， 1000ug砌l的ypd平板，以高濃度的抗生素篩選高拷貝的酵母工程菌，在含500ug ml高濃度抗生素平板上獲得了15個轉化子，取其中5個進行pcr擴增，有3個擴增產物與陽性對照相同，說明此酵母細胞中已含有s hbsag融合片段，其中之一命名為p
  3. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  4. The traditional homogene cloning method was modified on two aspects : first, homo - primers was designed at the sites with minimum degeneracy instead of maximum homology, secondly, some " n " bases were replaced with inosine in degenerate codes, resulting hi the degeneracy reduced from over 1000 times to below 100 times

    改進后的同源引物簡並度從1000倍以上降低至100倍以下，利用rt一pcr成功地擴增出一條630bp的鹽藻cdna片段，該片段與其它物種的烯醇酶基因具有很高的相似性。
  5. Two positive clones were sequenced, and the results showed that its nuclcotidc sequence includes an open reading segment which codes for a 45 - amino acids protein and three endonuclcase sites which arc1 bgii, bamh i and bgi ii, this protein was identified as metallothionein based on its characteristic described above and its similarity ( 85 % ) to the mtn gene of drosophila : the 10 cysteine residues present occur in five pairs of cys - x - cys, x is serine, valine, ilistidine or lysine

    結果顯示：擴增的cdna片段長度為289bp ，其中含有一個編碼45個氨基酸的開放閱讀框，閱讀框所編碼的氨基酸中含有10個半胱氨酸，且在序列中均排列成cys - x - cys ，其中x為ser 、 val 、 his或lys 。這些特徵說明擴增的基因片段為家蠅mt基因序列的一部分。此基因序列片段與果蠅mtn基因序列的同源性達到85 . 0 ，擴增的基因序列中含有三個內切酶位點bg 、 bam和bg ，這一點也和果蠅mtn基因十分相似。
  6. Compared with the 5. 8s complete sequence of the snail arion rufus, its1 and its2 regions were recognized and combined for analysis. from sequence observation, it showed that the zhejiang sample has more inserted sites and fragments while the sequences of other three are nearly all the same. the average g % + c % of the four individuals was 46. 8 % while the zhejiang sample ' s was 48. 3 % and the other three ' s were all about 46. 2 % ; ts / tv and genetic distance mainly lies between the zhejiang sample and the other three individuals, which were 0. 8 and 0. 07 respectively

    用於比較的序列長約350bp ，觀測一級結構，加拿大、墨西哥灣扇貝和美國二代個體的its1和its2序列幾乎完全相同，而浙江個體則具有較多的插入位點與片段； 4個個體平均g + c含量46 . 8 % ，其中浙江個體為48 . 3 % ，其它3個個體均為46 . 2 %左右；轉換顛換比與遺傳距離主要存在於浙江個體與其它3個個體之間，分別為0 . 8和0 . 07左右；以櫛孔扇貝作外群構建的分子系統樹表明：浙江群體已產生了一定的分化。
  7. So streptomyces sp. fr - 008 is a new strain synthesizing candicidin. nmr studies analysis of the 1h - 1h cosy spectrum allowed us to distinguish some important chemical shifts of the protons in fr - 008b which could be identified as eandicidin d. especially for amino - mycosamine residue, methyl protons and all the protons on the ring could be defined by correlating relation

    利用與糖基合成有關的基因str - de作探針對這兩個菌株的總dna進行了southern雜交，從兩個菌株的總dna中均檢測到一段相同大小的陽性片斷，約6 . 4kd （ bamhi + bglii酶切） 。
  8. Applying those engineering techniques, at present, the following results are obtained : the ground temperatures on the top and at the bottom of an embankment made of crushed rock are all lower than those of common embankment, so the crushed rock embankment is of great advantage of decreasing embankment temperature, and becomes an effective and initiative engineering measure to protect permafrost ; the crushed rock embankment can actively adjust the temperature difference between south slop and north slop, and mitigate the asymmetry of ground temperature ; the deformation of the crushed rock embankment is less than that of common embankment

    通過以上的施工技術，取得了相應的階段性成果：片石路基片石層頂面、底面與路基基底位置處地溫分別低於普通路基相同位置處地溫，片石路基有利於降低路基地溫，是一種有效的主動保護多年凍土工程措施；片石路基在調節路基陰陽坡地溫起到了積極的作用，減輕了路基地溫不對稱性的發生；對比片石路基和普通路基的路基變形量，片石路基的變形量相對較小。
  9. In this paper, phylogenetic relationship of 13 species involved in 6 genera of cruciferae wer e carried out through both the clones of homologous sequences with the primers designed on the basis of conserved regions of cyp86mf gene in cytochrome p450 gene superfamily and the differential analyses of them. meanwhile, complete sequences of some genes in cytochrome p450 gene superfamily were isolated and identified by smart pcr - race strategy, and expressed in e. colt. the results were as follows : ( 1 ) isolated by pcr from 11 species of cruciferae, eleven homologous gene segments that deduced amino acids were identities of over 80 % at nucleotide sequence level and similarities of over 70 % at amino acid sequence level

    本論文以已知的細胞色素p450基因超家族成員cyp86mf基因的保守區設計引物對十字花科重要蔬菜作物的6個屬13個物種進行了同源序列的分離克隆，通過核酸序列的差異比較分析，研究了該基因在不同物種中的進化關系；同時，通過保守引物的pcr擴增和race相結合的方法對十字花科植物不同物種的細胞色素p450基因家族成員基因全長進行了分離克隆、鑒定和原核表達的研究，獲得如下研究結果： （ 1 ）通過pcr從十字花科植物不同物種中擴增到11個可以推導出完整氨基酸序列的同源片段。
  10. It was interested that there was an extra six nucleosides insertion between 1647 - 1652nt ( according to the genomic sequence of la sota strain ), and the sequence were cccccc in f48e9 strain, and tcccac in zj1 strain. in order to test if insertion of this six nucleosides is related with the virulence of nd, two primers were designed to amplify the same fragment of another ten ndv strains. the result of sequence comparison of 16 strains showed that the six nucleoside was absent in lentogenic strain. this suggested that the six nucleosides insertion might have relationship with the ndv virulence. compared with all known sequence of ndv. there was a special sequence ( 5 ' tctctctcctctctcctcc3 " ) in the genomic cdna of ndv f48e9 strain

    通過rt - pcr方法擴增獲得了另外10個背景清楚毒株的np - p基因間隔區片段，將這些序列與f48e9 、 lasota 、 clone30 、 b1 、 zj1和v4的相應序列進行了比較，結果在參比的16個ndv毒株中在該區段中除了有多個點突變外，個別毒株有堿基插入和缺失，所有以lasota株為代表的弱毒株均無6堿基的插入，而以f48e9株為首的強毒株均有此6堿基的插入，但有一個中等毒力的毒株dp沒有6堿基的插入，不過它的基因序列和lasota的幾乎相同，對于所克隆到的基因的代表性還有待確定。
  11. In order to use the responding ability to the inducers of pr - la promoter, two fragments, ip1 ( 900 bp ) and ips ( 603 bp ) were cloned from tobacco genomic dna by polymerase chain reaction ( pcr ) with primers designed according to the sequence reported in genbank. sequence analysis of the amplified 900 bp fragment indicated that the cloned sequence had 99 % of homology to the known sequence. its transcription start site, tata box and consensus sequence " tgac " conserved in pr genes were identical to those of pr - la promoter

    根據pr - 1a基因的報道序列，設計兩對引物，以煙草基因組為模板，通過pcr擴增得到900bp （ ipl ）和603bp （ ips ）兩個目標片段，序列測定表明克隆的啟動子與報道序列具有99的同源性，轉錄起始位點、 tatabox及保守序列tgac與報道序列均完全相同。
  12. The sequences of intron, however, had only 74 % of homology with those of a. thaliana ban. in addition, amino acid sequence of oilseed rape ban had 78 % of homology and 87 % of similarity with those of a. thaliana and had various homologies ( 55 - 64 % ) with many nadph - dependent reductases of other plants

    將甘藍型油菜ban氨基酸序列進行同源比較，發現甘藍型油菜ban片段的181個氨基酸與擬南芥有78的氨基酸序列相同，相似性達87 ；與80多種植物的nadph還原酶類也有不同程度的同源性（ 55 - 64 ） 。
  13. By means of the afm made in shanghai aijian co., ltd. and digital instruments co., ltd ( u. s. ), the dna strand and their fragments were observed successfully. 2. by comparison with afm experiments of three categories of ion beams, the result has been observed in qualitatively : in the uniform dose, the higher let heavy ions have caused more double strand breaks than the lower let radiation

    通過afm對實驗結果的觀測，可以定性地得知：在同等劑量條件下，高let值的重粒子與低let的射線相比， dna雙鏈斷裂的數目要多一些；在相同let條件下，隨劑量增加dsb的片段變短、數量增加。
  14. Sequence analysis showed that, this fragment has a homology of 99 % to the previously reported choe from rhodococcus equi. after cloning and subcloning and three identical fragments were obtained from three independent pcr, lacking three continual bases ( ttc, encoding the pheamino acid ) compared with choe, thus it can be assumed that the new fragment, designated as choew, and choe belong to the same gene family, even it might be the natural mutation of choe

    對片段進行克隆、亞克隆之後測序分析，發現與已克隆的來源於馬紅球菌的膽固醇氧化酶基因cboe有99的同源性；並且三次獨立的pcr均得到相同的片段，所克隆的基因序列與choe相比，連續缺失三個堿基（ ttc ） ，即相應的氨基酸序列缺失一個氨基酸（ phe ） ，因此判斷所克隆的基因片段與choe屬同一基因家族或原有基因的天然突變體，命名為choew 。
  15. For the page to be identical, all markup fragments generated by the components that make up the page need to be identical

    頁面要完全相同，則需要構成該頁的組件生成的所有標記片段都完全相同。
  16. Part two : studies ; l. the sox gene of dinodon refozonatum was amplified by using a pair of primers which can amplify the conservative motif ( hmg - box ) of human sry gene. the amplification band was observed in both male and female dinodon refozonatum, whose length was consistent with that of human sry gene, which about 220bp. the result of sscp analysis showed that there were many differences in the sox gene sequence between dinodon refozonatum and human, and there was a few differences between male and female dinodon refozonatum. 2. using a pair of degenerate primers based on the conservative region, hmg - box, of human sry gene, six different fragments were amplified from either female or male dinodon refozonatum, then cloned by using pmd18 - t vector and sequenced

    結果顯示： （ 1 ）赤鏈蛇基因的擴增片段與人sry基因擴增片斷大小相同，為220bp左右； （ 2 ）雌、雄赤鏈蛇sox基因的擴增片段大小雖然與人的相同，但其單鏈遷移率與人的有較大差異，而且雌雄個體間有明顯差異，預示該基因的dna序列在雌雄個體中可能有差異； 2 、參照人sry基因hmg - box保守區的序列，又設計一對兼并引物，擴增了赤鏈蛇的sox基因，並對擴增產物進行了克隆和測序。
  17. The length of amplification was 140bp in both male and female, which was consistent with that of human dmrt1 gene. the result of sscp analysis showed that there were many differences in the dmrt1 gene sequence between alligator sinensis and human, but no differences between male and female alligator sinensis

    結果顯示： （ 1 ）揚子鱷dmrt1基因的擴增片段與人dmrt1基因擴增片段的大小相同，均為140bp ； （ 2 ）雌雄揚子鱷dmrt1基因擴增片段大小雖然與人相同，但其單鏈遷移率與人有較大差異，而雌雄個體間無差異。
  18. The clones inserted with ban fragments of b. napus and a. thaliana were sequenced and compared with the sequences in genbank. the results showed that the ban fragment of a. tha liana had the same sequence ( 779bp ) as reported. the ban fragment of b. napus was 780bp and its extron had 90 % of homology with those of a. thaliana ban ( 90 % )

    Pcr產物經克隆測序和比較分析顯示，擬南芥ban片段（ 779bp ）與已報道的完全相同，甘藍型油菜ban片段（ 780bp ）與擬南芥ban外顯子的同源性為90 ，但內含子的同源性僅為74 。
  19. The insert dna fragments are 7kb and llkb, respectively. two subclones that were designated pgr3h1 and pgr7h1 and can increase glyphosate resistance of e. coli jm109 up to 150mm glyphosate were constructed by subcloning the 2. 4kb and 3. 2 kb hind ? / psti fragments of pgr3 and pgr7 into the corresponding sites of pgem - 3zf and pbluescript. sequence analysis of these two subclones revealed a completely identical 1323bp open reading frame that encodes an epsp synthase

    以pgem - 3zf和pbluescript為載體，利用限制性內切酶hind和pst構建這兩個克隆的亞克隆，從中分別得到兩個草甘膦耐受亞克隆pgr3h1和pgr7h1 ，插入片段各為2 . 4kb和3 . 2kb ，對這兩個亞克隆進行序列分析，發現二者均含有一個核苷酸序列完全相同的完整的epsp合成酶基因? ? aroa ，其核苷酸序列長為1323bp ，推導的epsp合成酶由441個氨基酸組成，兩個亞克隆的草甘膦耐受濃度最大可達150mm 。
  20. Fragments generated by the primers ranged in size from 300 bp to approximately 3 kb. a total of 171 bands were amplified ranging from 1 to 16 bands each primer, 151 ( 88 % ) of which were polymorphic bands. the genetic distances between all pairs of test materials were calculated and phylogenetic relationships among them were discussed

    擴增出的片段分子量在0 . 3 3kb之間，條帶數在1 16條之間，共擴增出171個標記，樣品間相同的標記有20個，多態性標記有151個，多態性標記達80 。
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