receptor binding assay 中文意思是什麼
receptor binding assay
解釋
受體結合測定-
To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei, and to study the function and mechanism of mint - mediated transcription repression, yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5, amino acids 2226 - 2959 ). from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo, fifty - one were positive for nutritional screening and p - galactosidase assay. restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments, which are mint, alpha a - crystallin - binding protein i ( alphaa - crybpl ), and nuclear receptor co - repressor 1 ( n - corl ), respectively
鑒于mwt基因編碼區較長，共有10799個堿基，故此我們將mint分為六段，分別命名為fi一f6 ，本研究以其中的f5 （ 222e959 ）和f6 （ 296s576 ）片段為研究對象，將h者分別插入pgb盯7載體中，結果顯示： mintfs可與核受體輔助抑制因子1階cori ） ， o晶狀體蛋白結合蛋白1hlphatcrybp入及mw加互作用，而f6可與igm的重鏈恆定區、泛素結合酶2l6和一個未知功能的新的蛋白基因進行結合。 -
Methods receptor binding assay ( rba ) was adopted to measure the distribution of pgr and er in liver cancer and tissue in its vicinity in 63 patients with primary liver cancer ( plc ) undergone laparotomy and in non - tumor liver tissue in 7 cases with gastric carcinoma
方法採用放射性配基結合分析法（ rba ）測定了63例行手術切除的肝癌組織、癌周組織及7例非肝癌肝組織的雌、孕激素受體的含量並結合臨床資料進行統計分析。
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