replicative senescence 中文意思是什麼
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Light microtechnique and sa - galactosidase method was used to study the effects of super - high - concentration of glucose on the senescence of human diploid fibroblast 2bs cells, ros and the membrane potential of mitochondria during this process were measured. our results showed that 200 mmol l of glucose inhibited the growth of 2bs cells, led to the changes of reactive oxygen species and decrease of mitochondrial membrane potential, and caused senescence of 2bs cells rapidly. it supports the hypothesis of oxidative damage of senescence. moreover it is a better system for the study of the effects of ros during the process of replicative senescence
利用光學顯微鏡觀察和酸性-半乳糖苷酶染色技術研究了高濃度葡萄糖對人二倍體成纖維細胞2bs細胞衰老進程的影響，並用流式細胞儀檢測了此過程中活性氧和線粒體膜電位差的變化。結果表明： 200 mmol l的葡萄糖對2bs細胞有生長抑制作用，能引起活性氧含量的變化，導致線粒體膜電位差顯著下降，並誘導了細胞的衰老。這為氧化損傷假說提供了新的證據，並為研究活性氧和復制衰老之間的關系提供了較好的體系。
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