southern blotting 中文意思是什麼
southern blotting
解釋
southern印跡,dna印跡-
To verify the integration of st901 gene into transgenic plants genome, the stable transgenic plants were analyzed by pcr and southern blotting. the aberrant phenotypes were observed in pollens and anthers of the transgenic plants. most of pollen grains in the transgenic plant were distorted, shrunken, invagination and not stained with the solution of acetocarmine
通過對轉基因植株花粉、花藥形態的觀察和花粉活力的測定，表明st901啟動子驅動的st901基因在轉基因植株中的表達造成花粉嚴重敗育，花粉粒皺縮，扁癟、塌陷，缺乏內容物；轉反向表達載體的馬鈴薯花粉育性僅為對照的5 . 2 ，育性下降94 . 8 。 -
The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii. two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium. the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome. at the same time ; compared agrabactenum - mediated method with gene gun method, the transformation frequency of the former was 16. 7 %, while the latter was 50 %, so gene gun transformation method was suitable for long ya liiliwn
用攜帶有幾丁質酶基因和- 1 、 3葡聚糖酶基因的工程菌，通過農桿菌介導法和基因槍轉化法轉化龍牙百合，經pcr和點雜交檢測證明外源基因已經整合到植物染色體中。同時對農桿菌介導法和基因槍法進行比較，發現農桿菌介導法的轉化率為16 . 7 ，基因槍法的轉化率為50 ，因此可能基因槍轉化法更適于龍牙百合的遺傳轉化。 -
Objective through measure telomere length ( mean length of telomere restriction fragment, trf ) of dermal and intramuscular, and study it ' s length correlating with the different human " s age. the trf was examined by southern blotting. the formula to age estimating was obtained by regression analysis between the trf and the age
目的應用southern雜交技術，對皮膚、肌肉端粒dna片斷長度（ meanlengthoftelomererestrictionfragment ，簡稱： trf ）進行觀察，測定不同年齡段人群的端粒dna片斷長度值，以期初步探明不同年齡段人群的端粒dna片斷長度的變化規律，繪制出端粒dna片斷長度值隨年齡變化的標準曲線，比較性別、籍貫對端粒dna片斷長度的影響，以期為法醫實踐工作中對無名屍體年齡推斷提供理論依據。 -
Pllz1112 was transformed into m145 to disrupt sc6a9. 34. a mutant named hxy1 was obtained and confirmed to be disrupted at correct site by southern blotting
將phz1112導入野生型菌株m145中獲得基因中斷菌株hxy1並通過southern雜交驗證hxy1在正確位置發生了基因中斷。 -
They were coinjected into the male prenuclei of fertilized eggs with hsa. dna together. after that normal injected eggs were selected and transferred to the oviduct of pseudopregment recipents mice and gave birth to 65 fl offsprings, the foreign genes were found integrated in 12 of 65 mice by pcr and southern blotting detection
用顯微注射法分批把這三種不同的dna片段導人小鼠受精卵雄原核，並移植入假孕受體鼠，產生的65隻n代小鼠中，經pcr和southern雜交檢測，表明轉入的基因在12隻小鼠體內有整合段。 -
Pgxn217 was transferred into bradyrhizobium japonicum strain gx201 by triparental mating using the helper plasmid prk2073. marker exchange was achieved by selection on yma containing sm, km, gm, spc et al four kinds of antibiotics. mutants were confirmed by southern blotting and hybridization with the wild - type region and the tn5 fragment respectively
隨后，利用突變質粒pgxn217誘變慢生型大豆根瘤菌菌株gx201 ，獲得了gx201的突變體菌株gx217 ；將pgxn201的3 . 4kbecori片段與plarf3連接獲得亞克隆pgxn201cl ，然後將pgxn201cl導入突變體菌株gx217 ，構建了gx217的功能互補菌株rgx217 。 -
7 herbicide resistant plants were obtained. pcr, pcr - southern blotting analysis showed that the target genes were integrated into the plant genome. the segregation ratio in t1 of two examed transformants was 3 : 1
通過對標記性狀? ?除草劑抗性的活體及離體檢驗（氯酚紅法） 、目的基因pcr擴增、 pcr - southern雜交等方法證實目的基因已整合到小白菜基因組中。 -
89 ) via agrobacterium mediation. pcr and southern blotting analysis showed that the cmo and badh genes were integrated into tobaccos " genome. betaine accumulation in transformed tobaccos was higher than that in control plants
89 ） ，獲得卡那黴素抗性和潮黴素抗性植株， pcr和southern雜交均證明外源cmo和badh基因大連理工大學博士學位已整合到煙草基因組中。 -
When the seeds from to transgenic plants were cultured on medium supplemented with 50mg / l hygromycin, the ratio of germinated to non - germinated seeds is 3 : 1, which is consisted with mendel ' s first law. southern blotting demonstrated that the svde gene had been integrated into the genome of tobacco plants
轉基因植株的l代種了在潮黴素培養基上的萌發數與未萌發數的比值為3 ： l ，符合單基因的盂德爾分離規律。 -
Whether the gene was transformed into the arabidopsis was confirmed by pcr - southern blotting, southern blotting and northern blotting. 1. pcr - southern blotting showed that all kanamycin - tolerant plants had strong positive signals, and no signal was shown in wild type plants
Pcr結果擴增出1 . 4kb的特異性條帶，表明sod2已整合進擬南芥中， pcr - southern進一步證實了pcr結果的正確性。 -
Pcr analysis and southern blotting all confirmed that the ced - 9 gene has been integrated into the chromosomal dna of these transgenic plants
對所得到96株再生苗進行pcr檢測，結果表明，陽性苗比例為34 。 -
It indicated that 76. 5 percent of positive frequency of all the plants was achieved by using southern dot hybridization, 87. 5 percent of the plants with gus positive activity was gotten by pcr southern blotting and 42 percent of the plants with gus positive activity was gotten by southern blotting
轉基因卡那黴素抗性植株中， gus表達陽性率為43 ，隨機抽樣的方法進行殺蟲肽和npt基因的pcr檢測陽性率均為100 。經過分子生物學檢測表明， southern斑點雜交76 . 5植株表現陽性。 -
Three binary expression vectors were constructed, which harbored the cl - i - 2kl, r - 2kl, and d - l - 2kl - r - 2kl respectively, driven by the camv 35s promoter. then, agrofeac / erium - mediated transformation of iobacco ( nicotiana tabacum, w38 ) was carried out. transformed tobacco lines were obtained through tissue culture and confirmed by pcr and southern blotting analysis
通過分子克隆技術，將c1 - i - 2k1 、 r - 2k1和二者的連接（ c1 - i - 2k1 ? r - 2k1 ）分別構建到雙元表達載體上，並使它們分別置於35s啟動子的驅動之下，然後通過農桿菌介導的技術分別對煙草（ nicotianatabacum ， w38 ）進行了轉化，利用組織培養技術再生植株。 -
Phz1114 was transformed into the m145 and a gene replacement strain named hxy2 was obtained and testified by southern blotting
在mmasd及sfm上培養時發現： hxy2子代菌落有表型分離現象。 -
In the first part of this experiment, we isolated the tsmt gene ( genebank accession number : af499726. 1 ) from a a zap - cdna library cons tructed from a 200mmoll - 1 nacl - treated library of thellungiella salsuginea and analyzed its sequence characterization, genomic organization and the differential expression in response to salt stress. tsmt encodes metallothionein and there is only one copy in thellungiella salsuginea genome according to its southern blotting
L ~ （ - 1 ） nacl處理的鹽芥地上部分構建的zap - cdna文庫中克隆了編碼金屬硫蛋白的基因tsmt （ genebank號為af499726 . 1 ） ，並分別對其序列特徵、基因組結構和在鹽脅迫下的表達特性進行了分析，結果表明tsmt編碼鹽芥金屬硫蛋白， southern結果顯示tsmt在鹽芥基因組中只有一個拷貝。 -
Totally 99 transgenic rice plants from 125 resistant calli of 191 calli were obtained and pcr assay showed that 80. 3 % of them were positive. the result of southern blotting analysis for primary plants revealed that each transgenic plant contained a average of 2. 5 copy of t - dna
對抗性植株進行pcr擴增檢測，結果表明有80 . 3的抗性植株為陽性植株； southern雜交結果進一步證明了質粒的t - dna已經整合到水稻的基因組中，拷貝數為1 4個，平均每棵轉化植株有2 . 5個拷貝。 -
2 sequences were analysed and compared in genbank : ptfl is 494bp and ptf2 is 763bp in longth, the deduced proteins consist of 88 and 172 amino acids respectively, these cdna - clones encode the same gene - fe ( ii ) - transporter. 3 southern blotting showed that fe ( ii ) - transporter was a single copy in malus xiaojinensis genome and it was also existed in malus baccata and malus zumi
2 、根據測序的結果，將所得序列在genbank中進行同源性比較，結果如下： ptf1長為494bp ， ptf2長為763bp ，分別編碼88個和172個氨基酸，屬于小金海棠fe （ ）轉運蛋白基因的兩個不同長度的基因片段，是同一個基因。 -
Putative transgenic plants were screened b y nptii - specific and mnsod - specific pcr amplification and southern blotting, 84 % of the transgenic plants gave positive results. the results of mnsod activities demonstrated that tobacco mnsod gene contributed to more than 50 % of the total mnsod activity in some transgenic alfalfa
Npt基因和mnsod基因的pcr檢測和southern雜交表明mnsod基因已經整合到84的保定苜蓿轉基因植株的基因組中， mnsod活性測定結果表明轉基因植株中的mnsod活性與對照植株之間存在顯著差異，部分轉基因植株的mnsod活性比對照植株提高了1倍以上。
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