ss dna 中文意思是什麼
ss dna
解釋
然後由這些片斷連接成連續-
Constructing cdna expressing library of erythrocytic plasmodium falciparum from hainan : the total rna was obtained by using. triplix kit. a modified oligo ( dt ) primer ( cds ffi pcr primer ) was used in the single - stranded ( ss ) dna synthesis reaction. the ss - dna was reversely transcripted from total rna. double - stranded ( ds ) cdna was amplified by long - distance ( ld ) pcrafter the digestion with proteinase k and sfi i, the cdna with no less than 200bp was collected and purified by glass - milk kitthe library was constructed after the ligation of cdna to tiplex2 phage particle packaged with the packaging extract system in vitro. a high titer and high recombinant ratio of cdna library was constructed
構建惡性瘧原蟲海南株紅內期cdna表達文庫提取紅內期惡性瘧原蟲海南株總rna ,直接以總rna為模板使用cdna文庫構建試劑盒,首先反轉錄合成ss一dna ,再擴增合成ds一dna ( cdna ) ,對擴增產物用蛋白酶k消化及左z丁i酶切,抽提蛋白、去除rna后,用玻璃奶試劑盒純化、回收20obp以上的片斷,經與載體連接再用蛋白包裝物包裝后形成未擴增文庫,最後擴增完成惡性瘧原蟲海南株紅內期cdna表達文庫的構建。 -
And strain ss - ori is sinorhizobium sp. according to the " blast " data. in addition, a hydantoinase gene ( 1440bp ) from yz - ii6 was amplified by pcr with genomic dna as the template
我們還利用pcr方法從菌株yz - 6基因組中克隆得到了海因酶的基因( 1440bp ) ,並構建了表達質粒pet3a - hdtase 。 -
Two dna fragments encoding d - hydantoinase gene were amplified by pcr from chromosome dna of pseudomonas putida yz - 26 and sinorhizobium morelense ss - ori, respectively, and confirmed by dna sequence analysis
序列分析表明,來自yz - 26菌的d -海因酶基因全長1440bp ,編碼479氨基酸,基因序列已被genbank登錄( ay387829 ) ,被確定為一個新基因。
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