stage trapping 中文意思是什麼
stage trapping
解釋
多級分離-
The causes are discussed from aspects of oil - gas source beds, trapping and storing space, oil - gas migration channel and thick proofing layers, oil - gas maturity stage and allocation of effective trapping stage, suggesting the searching of primary oil - gas pool of the cambrian and lower ordovician at the relatively shallow layers and surrounding places with faults developed
從油氣源層、圈閉與儲集空間、油氣運移通道與巨厚隔層、油氣成熟期與圈閉有效期配置等四個方面探討了未獲油氣之原因，提示在滿加爾坳陷腹地，相對較淺部位及周邊斷裂發育部位尋找寒武系和下奧陶統的原生油氣藏。 -
Mature embryo - derived calli of japonica rice ( oryza sativa l. ) cultivars nipponbare were transformed using agrobacterium tumefaciens strain agl1 carrying a binary vector pcas04 harboring the marker gene, neomycin phosphotransferase gene ( nptii ), driven by a promoter from the ubiquitin gene in maize, a promoterless p - glucuronidase gene near to the left border of t - dna for trapping gene and a strong promoter, rice actin - gb promoter, near to the right border of t - dna as activation tagging. in this system, co - cultivation was simplified, special selection stages and pre - regeneration stage were omitted, the whole process was almost under continuous light at 30 ? except co - cultivation and transgenic plants began to generate only after 7 weeks calli were induced
在一步轉化系統中，光照高溫條件下培養的水稻愈傷組織從誘導開始經過4周時間就可以達到轉化實驗的要求，並且簡化、優化了整個共培養過程，省去了一篩、二篩和預分化步驟，只用7周的時間就可以初步得到再生轉化植株；共191塊愈傷組織得到125塊抗性愈傷組織，轉化頻率達到65 . 4 ，最後共得到99棵獨立來源的再生植株，抗性愈傷組織再生頻率達到79 . 2 。
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