staining plate 中文意思是什麼
staining plate
解釋
染色皿-
The assay system of the biological activity of lymphotoxin was established using l929 cell as the sensitive target, lt international standard as the positive control and crystal violet staining method to detect viable cell after treated with lt. the best relationship between dosage and effect could be got if the cell seeding density in cell plate was 1. 6 0. 1 104 the dosage of amd was lug / ml, and the starting concentration of dilution in the plate of lt standard was 10 iu / ml with two fold dilution. the credibility of the established system was detected with rhtnfp developed by r & d
為確定經上述步驟純化后得到的目的蛋自lt 27的生物活性，本研究以l929細胞為靶細胞、淋巴毒素國際標準品為參照，採用結晶紫染色法檢測經淋巴毒素處理后存活的細胞，對淋巴毒素生物活性測定的細胞接種濃度、淋巴毒素標準品板上稀釋的起始濃度和梯度稀釋的倍數、放線菌素d的使用劑量等進行條件實驗后，建立了人淋巴毒素生物活性測定方法。 -
During the course of purification, the method of orthophenanthroline staining is used to detect the ferritin. 30 | il of the fractions eluted from the aca ^ gel column were pulled into the cells of the minim incubation plate. then sopil 20 % dithionite and 40 | il 0. 1 % orthophenanthroline solution were pulled into respectively
在酶標板小孔中加入30 l待測液，加入30 l20的連二亞硫酸鈉溶液還原后，再加入40 l0 . 1鄰菲咯啉溶液，根據有無桔紅色顏色產生確定是否含鐵蛋白。
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