tag n 中文意思是什麼

tag n 解釋
標簽
  • tag : n 1 （衣服上的）垂下物，附屬物；帶端的金屬箍[包頭]。2 標簽；附箋，貼紙；（系在被監控的人或物上的...
  • n : 1. 【羅馬數字】90〈N＝90000〉。2. 【化學】＝nitrogen. 3. ＝North（ern）。N ＝nuclear 核的：N-waste 核廢料。
  1. The results shows that the vitro expressed protein of n gene by recombinant plasmid vector in the e. coli maintains anigenicity of tgev the recombinant protein was purified acconiing to the vector self characteristic ( hisk a polyhishdine tag introduced at the amino - acid terminus of the nucleoprotein allowed for the purification of protein by nickel - chelate dsity chromataography we explored all possibilities of pedcation and gained the modified purification method. several conditions, which include diffend ph buffer and concelltheion of imidazole, were selected to purify recombinan nucleorotein

    根據載體pproexhtb含有（ his ） 6特點，將融合蛋白進行純化，在純化過程中經各項條件的探索，確定為在裂解液中含有1mmpmsf的條件下，分別經過2倍體積的buffera和bufferb洗脫后，再收集ph5 . 9 ，含有80mmol / l咪唑的1倍體積bufferc洗脫液，可得到純化的融合蛋白。
  2. Both the mature genes of gloshedobin and gussurobin were cloned into the vector pet - 32a ( + ), strain bl21 ( de3 ), to study their expression in prokaryotic cell. the gene was expressed under t7 promoter with a fusion protein partner of thx. tag and a 6x his. tag at its n - terminal. having been induced by iptg for 4 hours, the recombinant enzyme was examined in the cytoplasm by sds - page analysis

    將大連蛇島蝮蛇和長白山白眉蝮蛇毒類凝血酶基因分別克隆到大腸桿菌表達載體pet - 32 （ a ） +中，在t7啟動子下表達出融合蛋白，融合部分為硫氧還蛋白，位於類凝血酶基因上游，並在其n端帶6xhistag標簽以利於表達產物的分離純化，經熱激轉化至宿主菌bl21 （ de3 ）中， iptg誘導斗小時后收獲菌體。
  3. On the other hand, our previous studies have shown that the nr2b construct with a gfp tag in its n - terminus cotransfected with nr1 subunit can reach the cell surface and be detected by surface staining with anti - gfp antibody in live cells. the nr2b construct will be retained in the endoplasmic reticulum ( er ) when expressed alone in heterologous cells

    我們以往的研究表明，用綠熒光蛋白（ gf內在信號膚下游n末端標記nrzb亞單位，與nri共轉染時能形成與野生型相似的nmda受體通道，且可以通過抗gfp抗體標記活細胞膜表面表達的nmda受體復合物。
  4. Passive tags do n ' t haven a power supply but a minute electrical current by an incoming radio frequency provides enough power for the tag to send a response

    無源標簽並無電源，由外來的無線電頻提供微電流，為標簽提供足夠電力以作回應。
  5. The former expresses recombinant proteins with a 6xhis tag at n - terminal. the fore mentioned four fragments were all used for expression in this system and the mature protein and the conservative domain were effectively expressed while the expression of the other two was unobvious

    前者表達n ?末端加6 his標記的融合蛋白，克隆到的4個基因片段均進行了表達，其中成熟肽和活性區域得到了大量表達，蛋白全長和末端缺失片段表達不明顯。
  6. A human lymphotoxin deletion gene fragment which lacking n - terminal 27 amino acid residues of the protein was pcr amplified using a pair of designed primers and cloned into expression vector pet36b ( + ) to construct a fusion protein with cbd tag, resulting a recombinant plasmid pet36b - lt 27. the recombinant plasmid was transformed into host e. coli bl21 ( de3 ) plyss

    採用pcr的方法擴增出人淋巴毒素n端缺失27個氨基酸的缺失體基因片段，將此基因片段克隆至表達載體pet36b （ + ）上，與t7lac啟動子控制下的cbdtag構建成表達融合蛋白的重組質粒pe736b - lt 27 。
  7. One of the most famous shows a bottle of chivas regal whisky without a label, and the tag line, " if you do n ' t recognize it, you ' re probably not ready for it.

    在事件發生之後，弗倫奇曾解釋說： 「女性工作者很難成為一位真正意義上的創意大師，因為她們要為照料孩子等家庭事務所拖累。 」
  8. Purification was conducted with ni - nta resin as there ' s a 6 x his tag at the n - terminal of the recombinant protein. after stepwise washing and elution at purification procedure, sublimated protein was obtained with the full - length ns5 accounting for more than 90 % and the aborted translated protein about 6. 5 %

    用ni一nta樹脂親和純化表達產物，經過梯度漂洗和洗脫，獲得純度90 %以上的全長表達產物，另有6 . 5 %的非全長翻譯產物（約70kda ） 。
  9. The other molecules ( li, chl1, po, tag, f3, prp ) did n ' t show any binding

    （ ）編碼bas … n和preb1山n的基因在屍rop基因敲除小鼠中水平下調。
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