template dna 中文意思是什麼
template dna
解釋
模板脫氧核糖核酸-
This time, using cdna of zmcdc5 as template, we amplify a sequence by means of pcr technology. and then, using restrict endoenzyme and ligase, we conjunct the 0. 8kb length dna sequence in a expression vector, pet - 30a. after induction, expression and purification, we obtained a 35. 4kda truncated fusing zmcdc5 protein which contains 267aa ( 647 to 914aa in zmcdc5 ). with the purified protein, we got its antibody and testified the antibody by means of western blotting and dot blotting
本實驗是以zmcdc5的cdna為模板，使用pcr獲得基因片段，再通過酶切連接，將得到的0 . 8kb的基因片段構建於pet - 30a表達載體上，經過誘導表達和純化，獲得zmcdc5的融合蛋白，其中包括了zmcdc5925個氨基酸中647 914共267個氨基酸殘基 -
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。 -
The template specificities found with dna polymerases from human leucocytes is summarized in table 1.
人白血病DNA聚合酶的模板特異性簡列于表1中。 -
The template specificities found with dna polymerases from human leucocytes is summarized in table 1
人的白血病dna聚合酶的模板特異性簡列于表1 。 -
At the same time, using the genomic dna of blast sensitive cultivar c039 as a template, two fragments were obtained that were the same large dna sequence as resistance cultivar c101a51. one sequence has conserved motifs of nbs - lrr type resistance genes, the other sequence can " t read through
同時本研究還以感病品種c039的基因組為模板，也擴增得到與c101a51抗病品種中抗病基因同源序列同樣大小的dna片段，但一個有抗病基因的保守結構，另一個片段不能通讀。 -
Used the genomic dna extracted by low melting - point agarose embedding method as pcr template, the full length of structural genes of bacillus subtilis bio operon were gained by long pcr method
將該方法提取的基因組dna稀釋100倍作為模板，採用長距離pcr方法，獲得了枯草桿菌生物素操縱子基因全長。 -
Among the three methods used in the experiment of dna extraction, only ctab, adding pvp in the dna isolation step, had effectively reduced the disturbance from fiber or other plastids and extracted suitable genomic dna as template for rapd process. pcr amplifications were performed in a final volume of 25 mm3 containing 0. 5 units taq polymerase, 2
通過在抽提緩沖液加入2的pvp 、並用異丙醇和乙醇沉澱基因組dna等改良措施， ctab法能避開大量纖維、多糖的影響，有效地從不同屬種的棕櫚科植物的幼嫩葉片中提取並純化了適合rapd的基因組dna 。 -
Introduction telomerase is a ribonucleoprotein reverse transcriptase that synthesizes telo - meric dna sequences using its rna as template. it can remedy the loss of te - lomere after cellular mitosis, maintain telomere length and stabilize chromosome
前言端粒酶（ telomerase ）是一種核糖核蛋白逆轉錄酶，能以自身的rna為模板，從頭合成染色體末端的端粒dna ，彌補細胞分裂時端粒dna的丟失，維持端粒的長度並穩定染色體。 -
Result a total of five primer pairs was designed to cover the whole mtdna control region and the neighbor part. the length of amplicon was from 299 bp to 452bp with different primer pairs. the successful result was obtained even if the dna template was small to 0. 015ng
結果設計了覆蓋整個mtdna控制區及周圍區域的5對引物，使各段擴增產物長度在299bp到452bp之間，統一了擴增條件使5段序列可以在相同循環參數下擴增。 -
Two oligonucleotides were synthesized according to the sequence of p. furiosus extracellular a - amylase gene amya through the genbank and used as primers for pcr with p. juriosus genomic dna as the template
根據genbank公布的p furiosus的-澱粉酶基因amya序列設計兩條引物，以p furiosus的基因組dna為模板進行pcr 。 -
Firstly, the hyaluronate synthase ( has ) gene ( hasa ) was cloned into the cloning vector puc19 by pcr using the total dna sample of s. equi as template
本文研究代謝工程理論，對本實驗室的一株ha生產菌（ streptococcusequi ）進行了ha合成與分解關鍵酶基因及功能的研究。 -
The dna encoding the desired protein was generated as a bamh i - sal i fragment using pcr. the template was pbv220 - hgf - a strand that had been constructed in our lab
其中gst是一種純化標簽，可以在後續的蛋白分離純化中使用gst親和層析的辦法純化目的蛋白，這將使純化工作變得簡單而有效。 -
Telomerase is a ribonucleoprotein complex ( rnp ) composed by its rna component and protein subunits. telomerase can synthesize telomeric dna onto chromosomal ends using its own rna component as a template, elongate the length of telomere, increase cell life and even induce cell immortalization
端粒酶（ telomerase ）是由端粒酶rna和蛋白質組成的一種核糖核蛋白復合物（ rnp ） 。端粒酶含有引物特異識別位點，能以自身rna為模板，逆轉錄合成端粒dna並加到染色體末端，使端粒延長，從而延長細胞的壽命甚至使其永生化。 -
Extraction of large - fragment genomic dna in order to gain dna template of pcr amplification ( long pcr amplification and salvage pcr amplification ) which was high purity and large fragment, three methods were used to extract genomic dna of bacillus subtilis, i. e. low melting - point agarose embedding method, sds - proteinase k - phenol chloroform extraction method and bacterial genomic dna extraction kit method. the genomic dna of bacillus subtilis were gained by these methods, and the operated programs of the methods were improved. the results showed that the genomic dna extracted by low melting - point agarose embedding method were obviously biggest than that of another two methods
大片段基因組dna的提取為了獲得用於pcr擴增（長距離pcr擴增和分段pcr擴增）的高純度、大片段（至少為pcr產物長度的4倍）的dna模板，應用三種方法：低熔點瓊脂糖包埋法， sds -蛋白酶k -酚氯仿抽提法和細菌基因組dna提取試劑盒法，分別提取獲得了枯草桿菌基因組dna ，並對3種方法的操作程序進行了不同程度的改進，結果表明：低熔點瓊脂糖包埋法提取的基因組dna片段明顯大於后兩種方法，採用0 . 5瓊脂糖凝膠電泳3h ，仍然跑不出加樣孔。 -
2. cloning of structural genes of bacillus subtilis bio operon diluted the genomic dna of bacillus subtilis as the template, long pcr product ( 10. 3kb ) and three salvage pcr products were separately gained by optimization of reaction conditions of pcr
枯草桿菌生物素操縱子基因的克隆將枯草桿菌基因組dna稀釋后，通過pcr反應條件的優化，分別擴增得到了生物素操縱子基因的長距離pcr產物（ 10 . 3kb ）和3個分段pcr產物。 -
2. cloning and functional expression of a heterogenous gene from a. nidulans in e. coli strain a. nidulans chromosome dna was used as template to amplfy gene qutb by pcr, the dna sequencing showed that the cloned gene was in agreement with the reported sequence
異源奎尼酸脫氫酶（ qdhase ）在大腸桿菌中功能性表達以構巢麴黴菌（ a . nidulans ）基因組dna為模板，用pcr方法擴增得到了qutb基因，序列測定與文獻報道一致。 -
2n methods 1 ) sample preparation the supernatant of cultures were digested with identical volume lysis buffer at 100 for 15 min. dna was isolated for use as template
二、實驗方法1 、標本處理取hcmv低傳代分離株細胞培養上清液與等量裂解液混勻后，煮沸15分鐘，提取dna ，作為擴增模板。 -
The packaging of eukaryotic dna into chromatin influences various processes that utilize dna as a template, including transcription, replication and repair
真核生物dna緊密包裝成染色質結構，影響了包括轉錄、復制和修復等在內的以dna為模板的每一個生物過程。 -
A pair of primers was designed based on the conserved regions of other higher plants " epsp synthase through homology alignments. two dna fragments were first cloned from o. violaceus by performing prc which used o. violaceus genome as the template. one has 798 nucleotides and the other 1157 nucleotides, but they can encode the same amino acid sequence and have same extrons according to the gt - ag rule of characteristic sequence of enkaryoutic intron
根據同源比較其它高等植物中epsp合成酶基因，找出該基因的保守序列並設計一對寡核苷酸引物，以諸葛菜的總dna為模板進行pcr反應，克隆出了兩個epsps基因的片段，其中一條長為797bp ，另一條1157bp ，它們在genbank的登錄號為： af440390 、 af440391 。 -
This has resulted in the availability of genetically modified l. lactis with prospects for application in food industry. in this studay, genomic dna extracted from lactococcus lactis nizo r5 was used directly as the template for pcr in this paper
乳酸乳球菌是一類長期應用於食品發酵業的有益微生物，以乳酸菌為受體的表達系統具有安全、表達產物便於分離，使用方便等優點。
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