template rna 中文意思是什麼
template rna
解釋
核糖核酸模板-
The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。 -
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。 -
Introduction telomerase is a ribonucleoprotein reverse transcriptase that synthesizes telo - meric dna sequences using its rna as template. it can remedy the loss of te - lomere after cellular mitosis, maintain telomere length and stabilize chromosome
前言端粒酶（ telomerase ）是一種核糖核蛋白逆轉錄酶，能以自身的rna為模板，從頭合成染色體末端的端粒dna ，彌補細胞分裂時端粒dna的丟失，維持端粒的長度並穩定染色體。 -
Linearized full - length cdna was used as template then genomic rna of csfv was in vitro transcriped by t7 rna polymerase
以線性化的全長cdna為模板，體外轉錄得到了csfv基因組rna 。 -
Telomerase is a ribonucleoprotein complex ( rnp ) composed by its rna component and protein subunits. telomerase can synthesize telomeric dna onto chromosomal ends using its own rna component as a template, elongate the length of telomere, increase cell life and even induce cell immortalization
端粒酶（ telomerase ）是由端粒酶rna和蛋白質組成的一種核糖核蛋白復合物（ rnp ） 。端粒酶含有引物特異識別位點，能以自身rna為模板，逆轉錄合成端粒dna並加到染色體末端，使端粒延長，從而延長細胞的壽命甚至使其永生化。 -
To device a primer pairs and amplify the full length pstvd by rt - pcr, the positive rna extraction from tuber sample was used as template. the product of rt - pcr was purified and connected to the plasmid pmd 18 - t vector
Cdna核酸斑點雜交反應（ nash ）檢測pstvd方法準確、靈敏度高，一次檢測樣品數量多，且對于異地樣品檢測非常方便，是以往其它檢測方法的有效補充。 -
A complete round of transcription involves the recruitment of polymerase and general transcription factors to the promoter, rna chain synthesis initiation and polymerase escape form the promoter, rna chain elongation, and finally termination with relaease of polymerase and nascent transcript from the dna template
一個完整的轉錄循環包括ranp和通用轉錄因子被招募至啟動子、 ran鏈合成的起始和ranp從啟動子的脫逸、 rna鏈的延伸以及伴隨ranp和新生rna鏈從dna模板釋放的轉錄終止等過程。 -
The ns5 protein of dengue virus is the largest molecule encoded by the virus genome with a molecule weight of 104 000. recent research work indicates that the ns5 protein acts as the rna - dependent rna polymerase ( rdrp ) in virus which has the ability to recognize and bind its template rna to synthesize a complementary strand
近年來的研究表明， ns5蛋白具有rna依賴的rna聚合酶（ rdrp ）功能，即可以識別具有相對特異性的模板rna並與之結合，合成與模板互補的rna鏈，在病毒基因組復制過程中起關鍵作用。 -
After transcription, we gained the subgenomic rna that may play the role of the template rna recognized by den rdrp
經轉錄反應獲得den亞基因組rna ，即為denrdrp可特異識別的模板rna 。 -
[ methods ] by using the overall rna of our previously cultured human melanoma cell line ( a375 ), full length fasl gene is detected by rt - pcr. using the cdna as template, . the extracellular domain of fasl ( fasl - ecd, 127 - 278aa ) is amplified by pcr. the pcr products are directly cloned into t vector pmd - 18t
L方法採用新鮮人黑色素瘤細胞（ a375 ） ，抽提該細胞的總rna ，進行rt一pcr反應分析a375內fasl全長編碼基因的轉錄表達，以a375細胞cdna為模板，用pcr產物直接克隆法擴增人fasl一ecd （人fasl胞外區）的編碼基因，即127一278位氨基酸殘基，而後將pcr產物直接克隆于pmd一1st載體中，獲得重組質粒pmd一t - fasl一ecd ，進行dna測序。 -
We concluded that excessive expression of exogenous htr gene may compete with the endogenous telomerase rna and prevent rna template from combining with telomeric dna, thus repressing the elongation of telomeric dna ( telomeres ) and causing cell aging and cell death. - 6 - 3. some modifications have been made to overcome the limitation of conventional telomeric repeat amplification protocol ( trap ) assay
分析其原因，可能是htr基因的過表達在數量和空間效應上同細胞內的端粒酶rna組分產生竟爭，一定程度上阻礙了端粒酶rna模板區與端粒dna的結合，從而抑制端粒dna的延伸，導致細胞凋亡。 -
The cdna encoding growth hormone ( gh ) peptide was amplified by reverse transcription polymerase chain reaction ( rt - pcr ) method using isolated total rna as template
應用逆轉錄-聚合酶鏈式反應（ rt - pcr ）技術克隆得到編碼草魚生長激素（ cgh ）的基因cdna ，並定向克隆到puc18載體上。 -
Bovine follistatin cdna gene was cloned by rt - pcr using total rna as template, which was extracted from bovine ovary by trizol total rna extract kit
本實驗從牛的卵巢中用trizol提取總rna ，並通過rt - pcr （反轉錄pcr ）的方法擴增獲得follistatincdna 。 -
Main works and their important results are showed as following. firstly, the cdna encoding bullfrog gh is amplified by use of the total rna, extracted from adult bullfrog ' s pituitary, as the template, pi ( 5 ' - cggatcc atg gct tca ggg tta ggc ) and p2 ( 5 ' - cgaattc tta aaa ggt gca gtt gct ) as the primer pair and one particular cdna product, 660bp, was obtained after the rt - pcr - the product was purified by using dna agarose gel extraction method. after the purified cdna and pmd18 - t vector were ligated at 16 over night, the ligation products were transformed into e. coli strain dh5a and then the transformants were screened by clone pcr method
首先，以成體牛蛙的腦垂體總rna為模板，進行rt ? pcr擴增得到約660bp的特異性pcr產物帶，切下瓊脂糖凝膠中的特異產物帶進行cdna膠回收，將cdna與pmd18 ? t載體進行t ? a克隆連接並轉化到dh5 e . coli后，進行菌落pcr 、質粒酶切鑒定，篩選出陽性菌株，測序結果經blast分析，與已報道的牛蛙生長激素基因高度同源，證實此陽性克隆為牛蛙生長激素基因轉化子，命名為pbfgh 。 -
The special primers were designed and synthesized after the analysis to the nucleotide sequence homology of etrl recorded in genbank. the total rna extracted from latex was reverse - transcribed into first strain of cdna. the special amplification products ( cdna fragment ) were obtained from pcr when using the first strain of cdna as template
通過對genbank登錄的已克隆的植物etr1基因序列進行核苷酸同源性分析，設計特異引物。以膠乳總rna為模板，反轉錄成cdna第一鏈，又以cdna第一鏈為模板進行pcr擴增，得到特異擴增片段。 -
To investigate the silence effect of hela cells " telomerase gene expression after shrna based on human telomerase htert transfected into cells. methods : we constructed a partial double - strand dna with t7 promoter as dna template and synthesized small hairpinrna in - vitro using t7 rna polymerase
方法：根據端粒酶htert基因1573 ? 1591位的核酸序列，構建帶t7啟動子的部分雙鏈dna模板，用t7rna聚合酶體外合成短鏈shrna 。 -
Conclusions : the in - vitro method that partial double - strand dna with t7 wi l. - toi promoter sequence as template and synthesizing by t7 rna polymerase can product high yield, excellent purity shrna. lt is a convenient -, effective ^ low - cost method and fit for small rna synthesis and rna interference researches in ordinary laboratory
結論：以帶t7啟動子部分雙鏈dna為模板，用t7rna聚合酶體外合成出的shrna產量較高，純度較好，是一種簡便、高效、低成本的短鏈rna的制備方法，適合於普通實驗室用來進行短鏈rna的合成和rna干擾實驗。
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