transfection 中文意思是什麼

transfection 解釋
轉變感染
  1. The protective effect of bcl - 2 gene transfection on heat stressed cardiomyocytes

    2基因轉染對熱應激心肌細胞的保護作用
  2. Protective effect of c - fos antisense gene recombinant transfection on ischemic hypoxic cardiomyocytes

    反義基因轉染對缺血缺氧心肌細胞的保護作用
  3. By co - transfection of the mutants with full - length raplgap, we found the mutant at 82 position could still bind to full length raplgap, while the mutants a

    本文採用a衛中標記的gtp與純化的rapla共孵育，使rapla全部與放射性同位素標記的gtp結合。
  4. The effectiveness of the nuclear localization signal selected was clearly tested in initial experiments. based on this, the co - transfection systems used in the study was established. we first observed the multinucleate cells and chromatin bridges in cultured hela cells when the cells are marked with gfp and hochest33342, after co - transfection with the gfp expression plasmids and rnai plasmids rhe or rhc

    當分別共轉染附加kozac序列和核定位信號的gfp與人集縮素smc亞基hcap一e特異的rnai質粒rhe和人集縮素smc亞基hca屍一c的f { nai質粒rhc時，都觀察到gfp和hochest33342標記的轉染后hela細胞表現多核和染色質橋現象。
  5. Transfection efficiency comparison of cationic liposome - dna complexes and lipid - protamine - dna complexes in vitro

    復合物體外細胞轉染率比較
  6. We also conducted the co - transfection with the rnai plasmids rhe and rhc and the selective marker gfp expressing plasmids simultaneously. there are three kinds of combination probability that could be observed by gfp marker

    在實驗中同時共轉染人集縮素smc亞基hcap一e和hcap一c特異的rnai質粒rhe和rhc和pcdna3 . 1 + kg到hela細胞。
  7. This report explored the function of the human smc subunits - hcap - e and hcap - c using rnai and transfection techniques. we constructed two rnai plasmids - rhe and rhc that specifically corresponded to hcap - e and hcap - c of human condensin, respectively

    為此我們構建了對人集縮素中兩個smc亞基hcap - e和hcap - c特異的rnai質粒rhe和rhc 。
  8. They are combination of co - transfection with rhe and gfp expressing plasmids, co - transfection with rhc and gfp expressing plasmids and co - transfection with plasmids rhe and rhc and gfp expressing plasmids at the same time. whichever the combination may be, we always observed the phenomena of chromatin bridge in co - transfected cells. these results indicate that the phenomena of chromatin bridge should be the characteristic feature of deficiency in the human smc subunits

    這時，存在三種可能的情況，即共轉染rhe和pedna3 . 1 * kc 、共轉染rhc和pedna3 . 1 + kg 、共轉染rhe和rhc和pc洲a3 . 1 + kg ，但都觀察到明顯的染色質橋現象，進一步證明這種表型是人集縮素中smc亞基的缺陷的特徵表型。
  9. The recombinant baculovirus harboring the appa gene was obtained after co - transfection and several screening rounds

    用乳糖誘導使appa基因在大腸桿菌菌株bl21中得到了高效表達。
  10. 3. polyethylenimine ( pei ), a water - soluble cationic polyelectrolyte, has been extensively used as a deformable and mobile substrate for a biomembrane for it high transfection efficiency of chemical - based delivery systems. what ' s more, pei is good buffer in physiological environment ( ph < 9 )

    3 、聚乙烯亞胺（ pei ）是一種陽離子型的聚合物，由於它在動植物細胞內優良的轉染效率，而被廣泛的應用，並且在生理環境下（ ph 9 ）它具有很強的緩沖能力。
  11. Conclusion : dendritic cells ( dcs ) can be derived from peripheral blood monocytes in vitro successfully treated with rhgm - csf of 1000 u / ml plus il - 4 of 500 u / ml. second, dcs from the patients of advanced carcinoma of large intestine were transfected with rv - cea, and then cocultured with autologous t cells. the cytotoxic activity against cea - secreting tumor cells were assessed and compared with those of t cells induced by dcs without rv - cea transfection

    其次，用rv - cea轉染晚期大腸癌患者的dc ，再激發自體t細胞，然後檢測t細胞對cea分泌性腫瘤細胞的體外殺傷活性，並與未經rv - cea轉染的dc激發的t細胞及野生型痘苗病毒轉染的dc激發的t細胞進行對比。
  12. Transfection of cell lines impaired for vzv infection with a plasmid expressing human ide resulted in increased entry and enhanced infection with cell - free and cell - associated virus

    Vzv感染受抑制的細胞系通過質粒表達轉染人類ide蛋白后可增加游離病毒顆粒以及細胞內病毒引起的感染。
  13. Cationic polymer mediated plasmid encoding egfp retrograde transfection in retinal ganglion cells

    基因逆行轉染視網膜神經節細胞
  14. Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f. for the construction of transfer vector pfgs11haf, aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11. recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l. recombinant rfpvs were derived by dosper liposome - mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3 - 4 hours earlier

    Puc18ha和sk質粒同時經hind 、 kpn酶切后連接得中間質粒skha ；將質粒skha用bamhi酶切回收ha基因插入到插入載體pfgs11中的bamhi位點，通過酶切鑒定獲得了pfgs11ha ；將含ndvf基因的質粒puc19f用hind 、 sal酶切經klenow酶補平插入到經sma酶切后的skifn中pe / l啟動子下獲得中間質粒skf ，再將質粒skf和puc18質粒先分別用ecor 、 xho酶切klenow補平，后再共同用sac酶切連接得puc18pelf ， sal酶切回收pe / l - f基因盒插入到pfgs11ha的sal位點，通過酶切鑒定獲得了pe / l - f與ps - ha同向的表達載體pfgs11haf 。
  15. These results indicate that the alteration of cell proliferation and dna synthesis caused by different gnt - v cdna transfection may at least partly result from the modification of n - glycan structure and function of egfr. it seems that the increased 1, 6 glcnac branch on the n - glycans of egfr may benefit to its binding with egf and the resulting tyrosine auto - phosphorylatio n, while the decrease of this branch may prevent these processes

    用特異性抗體結合westemblot結果發現，正義或反義gnt一vcdna的轉染並不引起pkb 、 p44 / 42mapk和mek蛋白質表達的變化，而gntv一s / h 」 21細胞pkbt308 、 5473位點磷酸化和免疫沉澱pkb的酪氨酸磷酸化以及以gsk召a /日磷酸化為指標的pkb的活性都較mock細胞增加， gntv一as / h7721細胞中這些指標的變化則相反。
  16. Are the changes in signal transduction and gene expression related to the structural alteration of the sugar chain on some surface receptors resulting from the transfection with sense or antisense gnt - v

    而細胞信號轉導的改變是否與細胞表面某些受體的糖鏈結構的改變（由gnt - v的正義或反義cdna轉染引起的）有關
  17. Part ii effects of gnt - v on the phosphorylation and activity of egfr signaling molecules in h7721 cells modification of the n - glycan structure and function of egfr by the transfection of different gnt - v cdnas may lead to the alterations of the phosphorylation and activity in the downstream molecules of signal transduction

    Gntv一s / h7721細胞p42 / 44mapk的磷酸化也較mock細胞增強，而gntv一as艦7721細胞則減弱，表明egfr的這兩條信號通路中信號分子的磷酸化或活力都受gnt一v的影響。
  18. To observe tsarg2 fusion protein expression in mammalian cell, pegfp - nl / tsarg2 fusion plasmid was constructed and transiently introduced into cos7 cell by liposome transfection. under the fluorescence microscope, the green fluorescence produced by pegfp - nl / rsarg2 was detected on the nucleus of cos7 cells after 24 hours post transfection, while the fluorescence produced by pegfp - nl was detected through the cells

    同時將21大、 35天、 49大、 280天小鼠睪丸進行組織石蠟切片，結果表明小鼠翠丸在第21天可見精子細胞，到第35天，精子生成的最後階段開始發生，部分牛精小管中可見少量成熟的精於。
  19. Direct damage on supf trna gene can be neglected because half - life of mnng is 1. 1 hour and the interval between treatment and transfection was as long as 12 - 24 hours. therefore the mutagenesis is not lesion directed

    20m的mnng經洗滌后的極微量殘留經過10 ? 20多個半衰期的降解后，己不足攻擊轉人的qdna分子，因此這種突變顯然是發生在mnng直接攻擊部位以外的正常堿基上。
  20. The supemate of cell culture was collected 48 hours after transfection. this supernate was also called conditional media

    在適當條件下收集尾蚴，體外培養使其脫尾變成童蟲。
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