transformant 中文意思是什麼

transformant 解釋
轉化體
  1. Transformation was done by electroporation. human fl extracellular domain cdna transformed to yeast host strain km71, then his + muts phenotype transformant was screened out and cultured in flasks, and rhfl was expressed under the induction of 0. 5 % methanol

    我們提取了km71ppic9k - fl轉化菌株的基因組dna進行southern實驗，檢測目的基因的整和插入；提取總rna ，進行了northern實驗，檢測fl基因在轉化菌株中的表達。
  2. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  3. The fermentation liquid of the transformant

    轉化子發酵液也具有脫毛效果。
  4. In contrast, most of the pollen grains in the non - transformant have plump shape and were stained into red color uniformly

    轉正向表達載體在煙草正常花粉粒的百分比較對照植株降低了42 。
  5. 16 replacement plasmids for two different regions were obtained, but have n ' t been introduced into 10 - 22 successfully. 42 cosmids in this region were introduced into a heterogeneous host zx64, one transformant exhibited a very faint inhibit activity against fusarium oxysporum when growed on a medium with thiostrepton

    將300kb區域的柯斯質粒進行異源表達，發現有一文庫質粒5h4的轉化子在硫鏈絲菌素存在時表現出對棉花枯萎病菌微弱的抑制活性；無硫鏈絲菌素存在時，則檢測不到。
  6. The transformant would flower in 8 to 10 weeks, sometimes under in vitro culture, after the leave dies was infected by agrobacterium tumefaciens

    轉化植株從浸染起8 - 10周可以開花，甚至在培養瓶中即可完成其生活史。
  7. And another change was that the spore of the transformant was difficult to release, p3a was not useful in genetically engineered strain construction in this way

    此外， pcr擴增發現轉化子比出發菌ybt - 1520多了cry1ab的特異帶， sds - page電泳證實cry1ab的存在。
  8. Confirmation of transformant. integration of sag - ipt into chrysanthemum genome was confirmed in two thirds of the putative tansgenic plantlet be pcr amplification

    通過pcr擴增表明，轉基因植株中可以檢測出有目的基因（ ipt基因）的存在，目的基因已成功地插入菊花的基因組中。
  9. Transformant ybt - 833 - 1 kept all indigenous plasmids, ybt - 833 - 2 lost a plasmid carrying crylab and ybt - 833 - 3 lost all indigenous plasmids carrying icp genes. comparing their potency with natural strain ybt - 833, the overall toxicity of all transformants was not noticeably higher than strain ybt - 833

    質粒pbmblc電脈沖轉入菌株ybt - 833 ，得到了幾個icp基因背景不同的轉化子，它們的綜合毒力較出發菌ybt - 833都沒能得到提高。
  10. Plant expression vector lba4404 ( pbin + - 35s - cbf3 + pal4404 ) was used in the genetic transformation of cucumber and micro - tomato. establishment of high efficiency regeneration system and determination of parameters involved in agrobacterium - mediated transformation, screening and characterization of transformant were systemically researched

    將植物表達載體pbin ~ + - 35s - cbf3對黃瓜和小番茄進行遺傳轉化，建立了黃瓜高頻再生和遺傳轉化體系，獲得了一株轉基因黃瓜植株，並通過了植物基因組pcr檢測；獲得了兩個具有kan抗性的小番茄再生綠芽。
  11. T. identification of charactrization of transgenic mustard plants the putative transformant regeneration plants were assayed by pcr and pcr - southern blot analysis. both analysis the target bands were observed. so the integration of the cpti gene into mustard genome dna was confirmed. the result of insect - resistance showed that the transgenic plants are more resistant than non - transgenic plants

    轉基因芥菜植株的鑒定取再生苗的葉片提取dna ，進行pcr擴增和pcrsouthernblot分析，轉化再生植株大部分呈陽性，而非轉化的再生植株均為陰性，證明cpt基因已存在於芥菜基因組中。
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