vaccinia virus recombinant 中文意思是什麼
vaccinia virus recombinant
解釋
重組痘苗簿- vaccinia : n. 【醫學】牛痘。adj. -l
- virus : n 1 【醫學】病毒；濾過性病原體。2 毒素；毒害。3 惡意，惡毒。4 【計算機】（電腦）病毒〈指擾亂或破...
- recombinant : n. 【遺】重組器官，復合器官。
-
Experimental study on mice immunized with recombinant vaccinia virus
重組痘苗病毒免疫小鼠的實驗研究 -
By using aa 385 - 365 fragment, an elisa system for the evaluation of post - e2 - vaccination humoral immune responses was also established, and was successfully applied to recombinant vaccinia virus - and dna - based vaccine research
利用aa385 - 565片段，建立了e2疫苗免疫后抗體反應的elisa評價體系，並已成功用於重組痘苗病毒疫苗和dna疫苗的研究。 -
Hi our study, dendritic cells ( dcs ) were derived from the cultivation of peripheral blood monocytes in vitro successfully. then to observe whether dcs transfected with carcinoembryonic antigen ( cea ) - vaccinia recombinant virus ( rv - cea ) can induces cytotoxic t lymphocyte - mediated cea - specific immunity in vitro
體外成功地完成了cd14 +單核細胞來源的樹突狀細胞（ dc ）的培養，並進一步研究人癌胚抗原重組痘苗病毒（ rv - cea ）轉染dc后體外誘導的cea特異性細胞免疫。 -
In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv
本研究採用脂質體轉染方法，將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞，經6輪蝕斑克隆、篩選、表達， pcr鑒定和dot - elisa檢測，證明該重組病毒已構建成功，並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。
分享友人