virulent vaccine 中文意思是什麼
virulent vaccine
解釋
有毒力疫苗-
In recent years, the outbreak of nd appear some new characters, it is reported that the amount of immunity defeat on nd increased, even chicks with high hi titer can be infected by ndv virulent strain. the gene - engineering vaccine is badly needed to control nd outbreak. so newcastle disease virus strain b95, a naturally avirulent and heat stable strain, was introduced from australia
新城疫是國際獸疫局公布的16種烈性傳染病之一。近年來，新城疫的爆發出現了許多新特點，免疫失敗報道增多，甚至抗體水平較高的雞群也發病。為控制新城疫的爆發，急需研製基因工程苗。 -
Hc ( hog cholera ), the virulent infectious disease caused by hcv ( hog cholera virus ), had been controlled effectively by hclv strain vaccine in the country
豬瘟是由豬瘟病毒引起的豬的一種病毒性傳染病。豬瘟兔化弱毒疫苗有效地控制了豬瘟在我國的急性發生和大流行。 -
The vaccine was developed by passaging eiav strain liaoning ( eiav l ) in donkeys in vivo, obtaining a donkey - adapted virulent equine infectious anemia virus ( eiav da ) and then passaging it in donkey leukocyte cultures for more than 120 times in vitro
該毒株是將eiavl株（ eiavl ）通過驢體傳代，使其毒力明顯增強，然後在驢白細胞培養物上連續傳代致弱獲得的。 -
But ib attenuated vaccine has many defects, such as the possibility of contamination of foreign and vertical transmissible pathogens, and the virulent recovery of vaccine virus. therefore, exploring the safe and efficient new vaccines has become the hotspot in the research of ib control
由於活疫苗存在外源性或垂直傳播性病原污染、毒力返祖等缺陷，開發安全、有效的新型疫苗是ib控制的研究重點。 -
The comparison of meq gene sequences of different pathotypes of marek ' s disease virus meq genes of different pathotypes of marek ' s disease virus ( mdv ) were amplified, in the whole opening reading frame ( orf ) by polymerase chain reaction ( pcr ) technique, sequenced and compared with the published sequence of ga strain ( representing vmdv ). cvi988 / rispens and 814, commercial vaccine strains popularly used worldwide and in china respectively ; 648a, representing very virulent plus ( vv + mdv ) and 6 field isolates, originated from guangxi commercial chickens with visceral lymphomas and vaccine breaks, representing high pathogenic ( hpmdv ) and two of them ( g2 and n ) were proved to be vvmdv, were used
本研究通過三個方面進行了研究，取得了如下主要實驗結果： 1mdv不同致病型（ pathotypes ）毒株meq基因序列的比較研究應用pcr技術擴增了不同致病型mdvs ，即國際通用型疫苗毒cv1988 rispens株和中國特有的疫苗毒814株、特超強毒648a株（ vv + mdv ）以及6個廣西分離到的野毒株共9個毒株的meq基因，進行核苷酸序列的測定並與標準強毒ga株（ vmdv ）進行核苷酸和氨基酸序列的比較。 -
The comparative analysis of sequences indicated that the sequences of meq in different pathotypes are relatively conserved and the homology of the amino acid sequences is very high. the significant differences include two mutations in both mdv - 1 vaccine strains cvi988 / rispens and 814 strain : the deletion of a proline ( no. l93aa ), and this mutation is just exactly located in a 15 - amino - acid ( eelcaqlcstppppi ) repeat sequence within the c - terminal transactivation domain of meq protein ; and a point mutation with a shift from alanine ( a ) of all virulent strains to serine ( s ) was occurred on the no. 71 aa
結果發現， mdv不同致病型的meq基因序列相對比較保守，它們相互間核苷酸和氨基酸序列的同源性均很高；但是，與所有七個致瘤性的mdv毒株相比，二個型弱毒疫苗cv1988 rispens株和814株均出現了兩個特徵性的突變：即第194位的p缺失性突變和第71位氨基酸由a變成了s的位點突變；缺失性突變恰恰位於meq基因中轉錄激活域內的一個多脯氨酸的重復序列（ pppp ）之中。 -
Four mcabs were developed and can detect the meq gene expression in the cef infected with oncogenic mdvs and naturally occurred md tumor cells by fa and immunohistochemistry technique respectively, but ca n ' t detect that in the cef and normal chicken liver tissue respectively infected with non - virulent vaccine virus cvi988 / rispens. the results demonstrated that meq is highly expressed in t
獲得4株穩定產生抗meq蛋白mcab的雜交瘤細胞，其中3g12e6單抗通過fa和免疫酶組化技術能夠檢測到mdv致瘤株感染的cef及自然md腫瘤細胞中表達的me叮基因產物，而在非致瘤株cvi988 / rispens感染的cef和免疫的正常雞的組織中則未檢測到。 -
The phylogenetic tree was constructed based on sequence comparison and analysis. the result showed that c strain derived from rabbit spleen tissue, c strain adapted to sk6 cell, shimen virulent strain all belonged to group 1. 1, and the prevalent virulent strains belonged to subgroup 2. 1 or subgroup 2. 2. this indicated there were at least two subgroups, which were prevailing in china, and the prevalent virulent strains were diverse far from c. strain vaccine virus
通過序列比較和系統發生關系分析發現： c -株兔組織毒、 c ?株細胞毒和中國50 60年代流行的石門強毒株同屬于組群1 （ group1 ） ；近期豬瘟流行毒株均屬于組群2 （ group2 ）的兩個不同的亞組群（ subgroup2 . 1和2 . 2 ） ，表明我國目前流行毒株至少有2個亞組群。 -
There were little difference in the amino acid composition of antigen domain of a, b and c between c strain from rabbit spleen tissue and virulent strains above. this indicated that the prevalence of csf and immunal failure may be not caused by the antigen variation of c stain vaccine virus. the e2 genes of 51 csfv prevalent virulent strains isolated from 12 provinces in china were amplified and sequencd
對c -株兔脾毒與c -株細胞毒、經典強毒e2上的a 、 b 、 c三個中和性抗原區的氨基酸組成進行了比較，其結果為： c -株兔脾毒與c -株疫苗細胞毒的差異很小甚至沒有差異。
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