western blotting 中文意思是什麼

western blotting 解釋
蛋白質印法
  • western : adj 1 西的，西方的；在西的；向西的；從西方來的。2 〈W 〉 西洋的，西歐的。3 日落西山的，衰頹的。n ...
  • blotting : 擦去油污
  1. This time, using cdna of zmcdc5 as template, we amplify a sequence by means of pcr technology. and then, using restrict endoenzyme and ligase, we conjunct the 0. 8kb length dna sequence in a expression vector, pet - 30a. after induction, expression and purification, we obtained a 35. 4kda truncated fusing zmcdc5 protein which contains 267aa ( 647 to 914aa in zmcdc5 ). with the purified protein, we got its antibody and testified the antibody by means of western blotting and dot blotting

    本實驗是以zmcdc5的cdna為模板，使用pcr獲得基因片段，再通過酶切連接，將得到的0 . 8kb的基因片段構建於pet - 30a表達載體上，經過誘導表達和純化，獲得zmcdc5的融合蛋白，其中包括了zmcdc5925個氨基酸中647 914共267個氨基酸殘基
  2. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  3. However, it is necessary to acquire the antibody or the antiserum, which could specially react with the expression protein of die objective gene transferred into the transgenic plant according to the characteristics of high homology and immune cross - reaction among plant ferritin, using the special immune serum of pea ferritin, the content of plant ferritin could be detected for studing the ferritin expression of transgenic plant by the technique of immunoassay such as immunoprecipitation, eljsa and western blotting

    利用免疫檢測技術進行植物轉基因的表達檢測是一種簡單、靈敏、快速、可靠的方法，但其前提條件是要有與轉基因植物目的基因表達的蛋白質發生特異性免疫反應的抗體或抗血清。根據植物鐵蛋白之間有高度同源性和交叉免疫反應的特性，利用特異性的豌豆鐵蛋白抗血清，就可通過免疫沉澱、 elisa或western雜交等免疫檢測方法進行植物鐵蛋白含量等的檢測，從而更好地進行轉基因方面的研究。
  4. Pupal hemolymph infected with recombinant virus bmpak - hbmp and bmpak - hbm showed four protein bands of about 31, ' 30, 28 and 25 kd in the western blotting profiles, due to the two atg initial codons at the 5 " ends of both pres2 and s gene

    Western雜交結果表明，感染bmpak hbmp和bmpak hbm的家蠶蛹血淋巴中均有4種不同分子量的蛋白質和鼠抗人的一抗發生免疫反應，大小約分別為31kd 、 30kd 、 zskd 、 25kd 。
  5. Sds - page and western blotting of plasmodia of physarum polycephalum showed that cyclin b was present in the plasmodia of s phase, g2 phase, prophase, and metaphase. the molecular weight of cyclin b in physarum polycephalum was around 62kda. cyclin b was gradually accumulated from s phase to metaphase, and reached the maximal level at metaphase, however disappeared in the plasmodia of anaphase and telophase

    Westernblotting分析結果表明， cyclinb存在於s期、 g _ 2期、前期和中期的原質團中，分子量約為62kda ， cyclinb的含量從s期到中期逐漸增加，特別是有絲分裂前期增加明顯，中期原質團cyclinb含量最高，后末期原質團中沒有cyclinb 。
  6. Sds - page results showed that there was a clear target protein band in mut + recombinant supernatant after 48 hours of culturing, while a faint band only in muts recombinant after 72 hours. western - blotting result showed that there was no remarkable difference of yield between mut + and muts recombinants after 6 days induced. anti - virus activity tests revealed that culture supernatants of mut + and muts recombinants could inhibit tmv infection with high efficiency in the same concentration and there was no significant difference between them

    結果表明，誘導培養48小時后， mut ~ +重組菌株表達產物在sds - page膠上顯現出清晰的目的蛋白帶，而mut ~ s重組菌株培養72小時才能顯示微弱的目的帶； western - blotting雜交信號強度表明，同樣培養6天的mut ~ +和mut ~ s重組菌株表達產物在表達量上沒有明顯差別。
  7. The valt and the p valt of cyanide - resistant respiration were induced dramatically as well as the total respiration in early stage of vernalization treatment and deceased subsequently. the western blotting results also showed the parallel changes. during the whole treament time of 0 - 30d, although the cyanide - resistant respiration was increased, its opearation was lower than the cytochrome pathway which also made the main contribution to total respiration of winter wheat

    結果表明:冬小麥胚芽在春化處理初期，伴隨著總呼吸速率的上升，抗氰呼吸容量（ valt ）和實際運行活性（ pvalt ）也快速升高，但在春化後期兩者均呈下降趨勢，與westernblotting結果十分吻合;在整個春化處理期間，抗氰呼吸活性雖有所上升，但相對于細胞色素主路來說，其運行還是處在一個較低的水平，而細胞色素主路則仍然占據了優勢。
  8. The amount of factor released from alginate calcium was determined ( n = 3 ) in western blotting

    定時采樣，以westernblotting法測量細胞因子釋放狀況。結果1
  9. The localization and expression of prolactin receptor from inner mongolia alpas cashmere goat were studied by sacpic staining, in situ hybridization and western blotting. samples of skin were taken at interval three months from birth, three months old, six months old, nine months old, ten months old or twelve months old, which correspond to summer, autumn, winter and spring. paraffin sections of hair follicles were stained with sacpic staining and in situ hybridization. the protein of prolatin receptor is abstracted from samples of skin in order to study on expression of prolactin receptor. there are prolactin receptors in outer root sheath, dermal papilla and inner root sheath. the growth of primary follicle is continuous

    本實驗從絨山羊出生后每隔三個月采一次皮樣，共分為4個月齡（ 3 、 6 、 9 、 10或12 ）段，通過製作石蠟切片，原位雜交、染色，並提取皮樣蛋白做westernblotting等實驗研究方法，研究了催乳素受體mrna催乳素受體在不同生長季節的內蒙古阿爾巴斯白絨山羊皮膚毛囊中的定位與表達，染色結果發現阿爾巴斯白絨山羊初級毛囊全年持續生長，次級毛囊的生長情況隨季節而變化，秋冬季生長旺盛，夏季生長緩慢與絨毛生成規律呈正相關。
  10. Interaction with doc - 1r and cdk2 proteins under physiological condition using cotransfection, coimmunoprecipitation and western blotting, we have transfected the plasmids into 293 cells according to following combination : pc

    分別將正、反義小鼠doc一ir重組體命名為pedna3一doc一ir +和pedna3一doc一ir一。
  11. The protein product of meq gene was highly expressed in the nuclei of recombinant baculovirus infected sf9 cells when using an anti - meq monoclonal antibody ( mcab ) 23b46 to run the immunofluorescence assay ( fa ) ; the expression quantity and if staining patterns differed with different times post - infection ( pi ). the results of western blotting and immunoprecipitation test showed there were two specific bands around 60 kd. the results of the study demonstrated that the baculovirus / insect cell system is effective to be used to express nuclear protein of virus

    結果發現:本表達系統產生的meq蛋白可被重組痘病毒表達的meq制備的單抗23b46所識別;在感染細胞中， meq蛋白僅局限於細胞核內，而且隨著感染后（ pi ）時間的增加，具有從核質向核仁和核膜轉移的趨向; w已stemblot和免疫沉澱試驗均證實重組桿狀病毒感染細胞裂解物中出現有兩條大小約為60kd的特異帶。
  12. 4. though tsa treatment and western blotting, we demonstrated that acetylation of certain non - histones may also be associated with the regulation of checkpoints in physarum polycephalum. 5

    由此認為，類c fos ，類c jun和類ras蛋白對多頭絨泡菌細胞周期各轉換點調控可能與細胞周期調控相關因於蛋白表達水平的變化有關。
  13. By treating the cells in s, g2 phase and prophase with histone deacetylase inhibitor tsa, and through the application of microscopic observation and western - blotting, we demonstrated that histone acetylation modification played important roles in the cell cycle regulation in physarum polycephalum, affecting the normal crossover of the checkpoints of s / g2, g2 / m and mitosis exit

    這些炎白的表達水平具有細胞周期依賴性，隨著細胞周期的進行而發生變化。 tsa處理引起的多頭絨泡菌s期、 gz期和前期細胞內組蛋白h3乙酚化水平的提高，改變了細胞內類cyclinbi蛋白、類。
  14. Methods : 1 ) 12h after irradiation, the cell cycle of nih3t3 cells was determined by flow of cytometry and the ratio of alternations in p16 gene exon - 2 was evaluated through pcr - sscp. 2 ) the content of mda, the activities of the sod and gsh - px in the supernatant of nih3t3 cells and the cells were measured by detecting kits immediately after irradiation. 3 ) the level of matrix metalloproteinase - 2 ( mmp - 2 ) in hela cells was detected by western - blotting and dot - blotting 2h after irradiation

    具體方法為： （ 1 ）照射后12h ，收集nih3t3細胞，用流式細胞儀檢測各組細胞的細胞周期， pcr - sscp檢測抑癌基因p16的變化； （ 2 ） nih3t3細胞照射后立即收集細胞和細胞上清，用試劑盒測量mda含量和sod 、 gsh - px的活性並觀察其變化； （ 3 ） western免疫印跡和點雜交法檢測照射2h后的各組hela細胞中基質金屬蛋白酶- 2 （ mmp - 2 ）的表達變化。
  15. Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid, and induced with methanol to secrete interesting peptide. the supernate of pichia pastoris culture was analysed by sds - page and western blotting. a reactive band, which the apparent molecular weight is 36kd, can be detected with sheep anti - hcmv polyclonal antibodies

    重組質粒轉化巴氏畢赤酵母， g418篩選出多拷貝插入的單克隆，甲醇誘導多拷貝插入的單克隆酵母細胞分泌目的蛋白，培養液上清經sds - page電泳分析，在蛋白質印跡中檢測到培養液上清有一表觀分子量為36kd ，能與羊抗hcmv多克隆抗體發生發應的條帶。
  16. The cultured cell suspensions tested by western - blotting showed that transfected cells could express the exogenous gene and secrete human lactoferrin protein, with mw of 34 kd. the highest amount detected with elisa reached 65mg / l medium / 105 cells. the recombinant hlf protein has the effect of inhibiting e. coli proliferation, whose activity is 1. 4 - 1. 8 times higher than the commercially available hlf

    誘導后，培養液上清通過western - blotting分析證明，轉染細胞表達並分泌出人乳鐵蛋白，分子量為34kd ； elisa檢測重組蛋白最高表達量為65mg l培養基10 ~ 5細胞；抗菌實驗表明，所獲得的重組人乳鐵蛋白具有抑制大腸桿菌生長的作用，而且比人乳鐵蛋白標準品作用更強。
  17. Western - blotting result demonstrated rhpf4 had specific reaction with rabbit anti - hpf4 antibody

    Western blotting檢測表達產物與兔抗人hpf4抗體發生特異的抗原抗體反應。
  18. In the first part of the present work, the expression changes of angiotensinogen ( agt ) and angiotensin - converting enzyme ( ace ) as well as its time course characteristics were investigated by rt - pcr, in situ hybridization and western blotting. we also prepared the polyclonal antiserum against agt for the following work

    故本工作應用rt - pcr和原位雜交、 western印跡分析等方法對模擬失重大鼠不同部位動脈血管血管緊張素原（ angiotensinogen ， agt ）及血管緊張素轉化酶（ angiotensin - convertingenzyme ， ace ）基因表達變化的時程特徵進行了觀察，並為后續工作制備了抗agt多克隆抗血清。
  19. In this paper, the effect of nuclear actin on the process of chromosome construction has been studied by utilizing the precise natural synchrous plasmodium of physarum polycephalum, sds - polyacryl amide gel electrophoresis ( sds - page ), western blotting, the cell - free system and optics microscopy. the major results and conclusions are as follows : 1

    本實驗以多頭絨泡菌原質團為材料，採用同步化培養、細胞核提取、 sds - page 、免疫印跡、非細胞體系構建、光學顯微鏡觀察等方法，研究了有絲分裂前期核內肌動蛋白對染色體構建的影響。
  20. Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells, we design a pair of particular primers, and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic. the expression plasmid was identified with pcr and dna sequencing. pbv220 - r hpf4 was transformed into e. coli dh5a, bl21 ( de3 ) and induced by increasing the temperature to 42. we identified the expression protein by sds - page and western - blotting

    目的1人血小板因子4 （ hpf4 ）原核高效表達克隆的構建2重組hpf4的表達及分離、純化工藝研究3重組hpf4的特性研究方法根據原核細胞表達真核蛋白的基因表達調控特點，設計合成一對特異引物，在pt7 - 7 - rpf4表達質粒的基礎上，應用聚合酶鏈式反應（ pcr ）對其cdna進行改造，通過dna重組技術構建成重組hpf4原核表達質粒pbv220 - rhpf4 ，用快速pcr檢測法、 dna測序分析，鑒定重組hpf4表達質粒的正確性。
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